##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AhdI Aor51HI AscI Asp718I AspEI
AvrII BbrPI BfrI BlnI BsaAI
BseAI BsiEI BsiWI BspDI BspEI
BssHII BssSI Bst1107I BstBI BstZ17I
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I FseI KpnI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NspV PaeR7I PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SmaI SnaBI SpeI
Sse8387I SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccII 5315 CGCG CG^CG
AgeI 3169 ACCGGT A^CCGGT
Asp700I 6020 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 5622 GGCGCC GGCGC^C
BcgI 3641 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 5384 GGATAC GTATCC(6/5)
BclI 5804 TGATCA T^GATCA
BglI 3485 GCCTGTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 4578 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 1211 GAATGC GAATGC(1/-1)
Bsp1407I 5536 TGTACA T^GTACA
BsrBI 5466 CCGCTC CCGCTC(-3/-3)
BsrGI 5536 TGTACA T^GTACA
BstUI 5315 CGCG CG^CG
DrdI 561 GACTCTCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 4658 GAATTC G^AATTC
EcoRV 2439 GATATC GAT^ATC
FbaI 5804 TGATCA T^GATCA
HaeII 5622 GGCGCC [GA]GCGC^[CT]
HpaI 811 GTTAAC GTT^AAC
KasI 5622 GGCGCC G^GCGCC
KspI 5314 CCGCGG CCGC^GG
MamI 4578 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 5315 CGCG CG^CG
NaeI 1602 GCCGGC GCC^GGC
NarI 5622 GGCGCC GG^CGCC
NgoMIV 1602 GCCGGC G^CCGGC
PacI 3074 TTAATTAA TTAAT^TAA
PinAI 3169 ACCGGT A^CCGGT
SacI 5888 GAGCTC GAGCT^C
SacII 5314 CCGCGG CCGC^GG
SfoI 5622 GGCGCC GGC^GCC
SgrAI 3168 CACCGGTG C[GA]^CCGG[CT]G
SspBI 5536 TGTACA T^GTACA
SspI 159 AATATT AAT^ATT
TaqI 2263 TCGA T^CGA
TthHB8I 2263 TCGA T^CGA
XbaI 2831 TCTAGA T^CTAGA
XmnI 6020 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 4541 ACATGT A^C[GA][CT]GT
5526 ACATGT
Alw44I 4420 GTGCAC G^TGCAC
4483 GTGCAC
ApaI 2800 GGGCCC GGGCC^C
5635 GGGCCC
ApaLI 4420 GTGCAC G^TGCAC
4483 GTGCAC
AspI 498 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2249 GACCTAGTC
AviII 1452 TGCGCA TGC^GCA
2241 TGCGCA
BamHI 1291 GGATCC G^GATCC
3046 GGATCC
BbvCI 3652 CCTCAGC CCTCAGC(-5/-2)
4945 GCTGAGG
BglII 1685 AGATCT A^GATCT
5298 AGATCT
BsaWI 3169 ACCGGT [AT]^CCGG[AT]
4816 TCCGGT
BsmBI 5232 CGTCTC CGTCTC(1/5)
3723 GAGACG
BspLU11I 4541 ACATGT A^CATGT
5526 ACATGT
BstXI 3795 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4565 CCAGTAAGCTGG
DraI 4375 TTTAAA TTT^AAA
5076 TTTAAA
DraIII 485 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1792 CACTTAGTG
EarI 3016 CTCTTC CTCTTC(1/4)
211 GAAGAG
EcoT22I 1232 ATGCAT ATGCA^T
5156 ATGCAT
FspI 1452 TGCGCA TGC^GCA
2241 TGCGCA
HindIII 3549 AAGCTT A^AGCTT
3839 AAGCTT
Ksp632I 3016 CTCTTC CTCTTC(1/4)
211 GAAGAG
NsiI 1232 ATGCAT ATGCA^T
5156 ATGCAT
PflFI 498 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2249 GACCTAGTC
PshAI 1248 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3446 GACCGAGGTC
Tth111I 498 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2249 GACCTAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
AseI AsnI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspHI
BspMI BsrDI BsrFI BsrI BssKI
BstAPI BstEII BstF5I BstNI BstPI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PshBI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XcmI XhoII