##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
Acc65I AclI AgeI Aor51HI AscI
AseI AsnI Asp718I AviII AvrII
BclI BlnI BsiWI Bsp1407I BsrGI
BssSI Bst1107I BstBI BstZ17I CpoI
DraI DraIII Eco47III FbaI FspI
HpaI KpnI MfeI MluI MunI
NheI NotI NruI NspV PacI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SalI SbfI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AatII 412 GACGTC GACGT^C
AccI 655 GTCTAC GT^[AC][GT]AC
AflIII 4154 ACATGT A^C[GA][CT]GT
AhdI 324 GACGGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 931 GTGCAC G^TGCAC
AocI 744 CCTGAGG CC^T[ACGT]AGG
ApaLI 931 GTGCAC G^TGCAC
AspEI 324 GACGGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 1440 CACGTG CAC^GTG
BcgI 3224 GCATGGATTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 196 GCCTGGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1440 CACGTG [CT]AC^GT[GA]
BsaBI 4311 GATTCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 5676 GCATTC GAATGC(1/-1)
BspLU11I 4154 ACATGT A^CATGT
BssHII 16 GCGCGC G^CGCGC
Bsu36I 744 CCTGAGG CC^T[ACGT]AGG
EagI 2003 CGGCCG C^GGCCG
Eam1105I 324 GACGGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2003 CGGCCG C^GGCCG
Eco52I 2003 CGGCCG C^GGCCG
Eco81I 744 CCTGAGG CC^T[ACGT]AGG
EcoT22I 3762 ATGCAT ATGCA^T
FseI 2004 GGCCGGCC GGCCGG^CC
HindIII 5448 AAGCTT A^AGCTT
KspI 1938 CCGCGG CCGC^GG
MamI 4311 GATTCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 4505 CATATG CA^TATG
NsiI 3762 ATGCAT ATGCA^T
PaeR7I 4678 CTCGAG C^TCGAG
PmaCI 1440 CACGTG CAC^GTG
PmlI 1440 CACGTG CAC^GTG
SacII 1938 CCGCGG CCGC^GG
ScaI 2762 AGTACT AGT^ACT
XhoI 4678 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccIII 31 TCCGGA T^CCGGA
992 TCCGGA
AflII 3747 CTTAAG C^TTAAG
4919 CTTAAG
ApaI 122 GGGCCC GGGCC^C
1024 GGGCCC
Asp700I 2427 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4950 GAATCCCTTC
BfrI 3747 CTTAAG C^TTAAG
4919 CTTAAG
BglII 1493 AGATCT A^GATCT
4654 AGATCT
BlpI 2286 GCTCAGC GC^T[ACGT]AGC
2979 GCTGAGC
Bpu1102I 2286 GCTCAGC GC^T[ACGT]AGC
2979 GCTGAGC
BseAI 31 TCCGGA T^CCGGA
992 TCCGGA
BspDI 1012 ATCGAT AT^CGAT
1390 ATCGAT
BspEI 31 TCCGGA T^CCGGA
992 TCCGGA
BspHI 449 TCATGA T^CATGA
3126 TCATGA
BsrBI 213 GAGCGG CCGCTC(-3/-3)
1927 GAGCGG
CelII 2286 GCTCAGC GC^T[ACGT]AGC
2979 GCTGAGC
ClaI 1012 ATCGAT AT^CGAT
1390 ATCGAT
DrdI 2262 GACAGGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2811 GACAGACAGGTC
EcoRV 445 GATATC GAT^ATC
4941 GATATC
HincII 1741 GTCAAC GT[CT]^[GA]AC
4456 GTCAAC
HindII 1741 GTCAAC GT[CT]^[GA]AC
4456 GTCAAC
MroI 31 TCCGGA T^CCGGA
992 TCCGGA
RcaI 449 TCATGA T^CATGA
3126 TCATGA
SapI 1405 GAAGAGC GCTCTTC(1/4)
5174 GCTCTTC
SmaI 110 CCCGGG CCC^GGG
473 CCCGGG
SspI 3896 AATATT AAT^ATT
3903 AATATT
StuI 1891 AGGCCT AGG^CCT
3699 AGGCCT
XmaCI 110 CCCGGG C^CCGGG
473 CCCGGG
XmaI 110 CCCGGG C^CCGGG
473 CCCGGG
XmnI 2427 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4950 GAATCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AspI AvaI AvaII BalI BamHI
BanI BanII BbeI BbsI BbvCI
BbvI BciVI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoO65I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflFI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII