##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AccIII AclI AgeI Alw44I ApaLI
AscI Asp700I AviII BbrPI BcgI
BclI BglI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BsrGI BstBI ClaI EagI
EclXI Eco52I EcoRV FbaI FseI
FspI HindIII KspI MfeI MluI
MroI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PaeR7I PinAI PmaCI PmeI PmlI
Psp1406I PvuI RcaI SacII SalI
SfiI SfuI SgrAI SnaBI SpeI
SspBI SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AatII 2076 GACGTC GACGT^C
AccI 904 GTATAC GT^[AC][GT]AC
AhdI 3436 GACCCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1207 AGCGCT AGC^GCT
AseI 5335 ATTAAT AT^TAAT
AsnI 5335 ATTAAT AT^TAAT
AspEI 3436 GACCCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI 1274 GTATCC GTATCC(6/5)
BsaBI 3975 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BssHII 226 GCGCGC G^CGCGC
BssSI 1905 CACGAG CACGAG(-5/-1)
Bst1107I 904 GTATAC GTA^TAC
BstZ17I 904 GTATAC GTA^TAC
CpoI 342 CGGACCG CG^G[AT]CCG
DraI 5527 TTTAAA TTT^AAA
DrdI 1450 GACGACTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3436 GACCCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1207 AGCGCT AGC^GCT
EcoRI 2028 GAATTC G^AATTC
EcoT22I 2092 ATGCAT ATGCA^T
HincII 1950 GTTAAC GT[CT]^[GA]AC
HindII 1950 GTTAAC GT[CT]^[GA]AC
HpaI 1950 GTTAAC GTT^AAC
MamI 3975 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NsiI 2092 ATGCAT ATGCA^T
PacI 5336 TTAATTAA TTAAT^TAA
PshAI 4398 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5335 ATTAAT AT^TAAT
RsrII 342 CGGACCG CG^G[AT]CCG
ScaI 2668 AGTACT AGT^ACT
SphI 4954 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflII 4798 CTTAAG C^TTAAG
5572 CTTAAG
AflIII 2916 ACATGT A^C[GA][CT]GT
3009 ACATGT
BalI 2188 TGGCCA TGG^CCA
3574 TGGCCA
BbeI 501 GGCGCC GGCGC^C
1857 GGCGCC
BfrI 4798 CTTAAG C^TTAAG
5572 CTTAAG
BsaAI 1351 TACGTG [CT]AC^GT[GA]
1432 TACGTG
BsaWI 1094 TCCGGT [AT]^CCGG[AT]
1713 ACCGGA
BspLU11I 2916 ACATGT A^CATGT
3009 ACATGT
BsrDI 3483 CATTGC GCAATG(2/0)
2775 GCAATG
BsrFI 2197 ACCGGC [GA]^CCGG[CT]
2218 ACCGGC
BstAPI 1530 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3694 GCAGCATCTGC
Cfr10I 2197 ACCGGC [GA]^CCGG[CT]
2218 ACCGGC
KasI 501 GGCGCC G^GCGCC
1857 GGCGCC
MluNI 2188 TGGCCA TGG^CCA
3574 TGGCCA
MscI 2188 TGGCCA TGG^CCA
3574 TGGCCA
NarI 501 GGCGCC GG^CGCC
1857 GGCGCC
PflMI 1633 CCACGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3489 CCAGGCACTGG
SexAI 908 ACCAGGT A^CC[AT]GGT
1520 ACCTGGT
SfoI 501 GGCGCC GGC^GCC
1857 GGCGCC
TfiI 2841 GATTC G^A[AT]TC
4644 GATTC
Van91I 1633 CCACGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3489 CCAGGCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
Acc65I AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI AocI
ApaI ApoI Asp718I AspHI AspI
AvaI AvaII AvrII BamHI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglII BlnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KpnI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflFI
PleI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I SbfI
ScrFI SfaNI SfcI SmaI SmlI
Sse8387I SspI StuI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XcmI XhoII XmaCI
XmaI