##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AatII Acc65I AgeI AhdI Alw44I
ApaLI AscI Asp718I AspEI AviII
BsaBI BsiWI BspDI BsrFI BssHII
Bst1107I BstBI BstZ17I Cfr10I ClaI
CpoI DraI Eam1105I EcoRI EcoRV
FseI FspI HpaI KpnI KspI
MamI MfeI MluI MunI NaeI
NdeI NgoMIV NotI NruI NspV
PacI PinAI PmeI PvuI RsrII
SacII SfuI SgrAI SnaBI SpeI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccIII 3466 TCCGGA T^CCGGA
AclI 4121 AACGTT AA^CGTT
AcsI 4362 GAATTT [GA]^AATT[CT]
AcyI 4067 GGCGCC G[GA]^CG[CT]C
Aor51HI 5574 AGCGCT AGC^GCT
ApoI 4362 GAATTT [GA]^AATT[CT]
AseI 4082 ATTAAT AT^TAAT
AsnI 4082 ATTAAT AT^TAAT
BamHI 4196 GGATCC G^GATCC
BbeI 4067 GGCGCC GGCGC^C
BbrPI 2486 CACGTG CAC^GTG
BciVI 918 GTATCC GTATCC(6/5)
BclI 1755 TGATCA T^GATCA
BglII 3040 AGATCT A^GATCT
BsaHI 4067 GGCGCC G[GA]^CG[CT]C
BsaWI 3466 TCCGGA [AT]^CCGG[AT]
BseAI 3466 TCCGGA T^CCGGA
BsiEI 766 CGGCCG CG[GA][CT]^CG
BspEI 3466 TCCGGA T^CCGGA
BspHI 4209 TCATGA T^CATGA
BsrBI 1186 GAGCGG CCGCTC(-3/-3)
DrdI 3992 GACATGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 766 CGGCCG C^GGCCG
EclXI 766 CGGCCG C^GGCCG
Eco47III 5574 AGCGCT AGC^GCT
Eco52I 766 CGGCCG C^GGCCG
FbaI 1755 TGATCA T^GATCA
Hin1I 4067 GGCGCC G[GA]^CG[CT]C
KasI 4067 GGCGCC G^GCGCC
MroI 3466 TCCGGA T^CCGGA
NarI 4067 GGCGCC GG^CGCC
PaeR7I 4243 CTCGAG C^TCGAG
PmaCI 2486 CACGTG CAC^GTG
PmlI 2486 CACGTG CAC^GTG
PshAI 1913 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4082 ATTAAT AT^TAAT
Psp1406I 4121 AACGTT AA^CGTT
RcaI 4209 TCATGA T^CATGA
SalI 3561 GTCGAC G^TCGAC
SapI 4484 GCTCTTC GCTCTTC(1/4)
SbfI 2845 CCTGCAGG CCTGCA^GG
ScaI 3538 AGTACT AGT^ACT
SexAI 841 ACCTGGT A^CC[AT]GGT
SfiI 1729 GGCCCCGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 4067 GGCGCC GGC^GCC
Sse8387I 2845 CCTGCAGG CCTGCA^GG
SspI 5212 AATATT AAT^ATT
XhoI 4243 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflII 1484 CTTAAG C^TTAAG
1610 CTTAAG
AflIII 535 ACATGT A^C[GA][CT]GT
4202 ACATGT
ApaI 3660 GGGCCC GGGCC^C
4730 GGGCCC
Asp700I 3038 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5211 GAATATTTTC
BbvCI 5131 CCTCAGC CCTCAGC(-5/-2)
3317 GCTGAGG
BcgI 4245 CGAGGGTGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4675 CGAGGTGTCTGC
BfrI 1484 CTTAAG C^TTAAG
1610 CTTAAG
BlpI 812 GCTCAGC GC^T[ACGT]AGC
3326 GCTCAGC
Bpu1102I 812 GCTCAGC GC^T[ACGT]AGC
3326 GCTCAGC
BsaAI 2486 CACGTG [CT]AC^GT[GA]
2643 TACGTG
BsaI 4595 GAGACC GGTCTC(1/5)
212 GGTCTC
BsmBI 2127 CGTCTC CGTCTC(1/5)
4602 CGTCTC
BsmI 4216 GAATGC GAATGC(1/-1)
4572 GCATTC
Bsp1407I 5249 TGTACA T^GTACA
5527 TGTACA
BspLU11I 535 ACATGT A^CATGT
4202 ACATGT
BsrGI 5249 TGTACA T^GTACA
5527 TGTACA
BstAPI 2388 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4822 GCAGTATATGC
BstXI 2998 CCATCGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3025 CCATCTCACTGG
CelII 812 GCTCAGC GC^T[ACGT]AGC
3326 GCTCAGC
DraIII 1803 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
5187 CACTGTGTG
EcoT22I 4930 ATGCAT ATGCA^T
5309 ATGCAT
HgaI 4305 GCGTC GACGC(5/10)
4799 GCGTC
HincII 3561 GTCGAC GT[CT]^[GA]AC
4088 GTCAAC
HindII 3561 GTCGAC GT[CT]^[GA]AC
4088 GTCAAC
HindIII 174 AAGCTT A^AGCTT
3642 AAGCTT
NsiI 4930 ATGCAT ATGCA^T
5309 ATGCAT
SspBI 5249 TGTACA T^GTACA
5527 TGTACA
XmnI 3038 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5211 GAATATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
AlwI AlwNI AocI AspHI AspI
AvaI AvaII AvrII BalI BanI
BanII BbsI BbvI BcnI BfaI
BglI BlnI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BssSI
BstEII BstF5I BstNI BstPI BstUI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NheI
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI SphI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII XmaCI XmaI