################################## # NO CUT ENZYMES ( 52 enzymes) # ################################## AatII Acc65I AgeI AhdI Alw44I ApaLI AscI Asp718I AspEI AviII BsaBI BsiWI BspDI BsrFI BssHII Bst1107I BstBI BstZ17I Cfr10I ClaI CpoI DraI Eam1105I EcoRI EcoRV FseI FspI HpaI KpnI KspI MamI MfeI MluI MunI NaeI NdeI NgoMIV NotI NruI NspV PacI PinAI PmeI PvuI RsrII SacII SfuI SgrAI SnaBI SpeI SwaI XbaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccIII 3466 TCCGGA T^CCGGA AclI 4121 AACGTT AA^CGTT AcsI 4362 GAATTT [GA]^AATT[CT] AcyI 4067 GGCGCC G[GA]^CG[CT]C Aor51HI 5574 AGCGCT AGC^GCT ApoI 4362 GAATTT [GA]^AATT[CT] AseI 4082 ATTAAT AT^TAAT AsnI 4082 ATTAAT AT^TAAT BamHI 4196 GGATCC G^GATCC BbeI 4067 GGCGCC GGCGC^C BbrPI 2486 CACGTG CAC^GTG BciVI 918 GTATCC GTATCC(6/5) BclI 1755 TGATCA T^GATCA BglII 3040 AGATCT A^GATCT BsaHI 4067 GGCGCC G[GA]^CG[CT]C BsaWI 3466 TCCGGA [AT]^CCGG[AT] BseAI 3466 TCCGGA T^CCGGA BsiEI 766 CGGCCG CG[GA][CT]^CG BspEI 3466 TCCGGA T^CCGGA BspHI 4209 TCATGA T^CATGA BsrBI 1186 GAGCGG CCGCTC(-3/-3) DrdI 3992 GACATGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 766 CGGCCG C^GGCCG EclXI 766 CGGCCG C^GGCCG Eco47III 5574 AGCGCT AGC^GCT Eco52I 766 CGGCCG C^GGCCG FbaI 1755 TGATCA T^GATCA Hin1I 4067 GGCGCC G[GA]^CG[CT]C KasI 4067 GGCGCC G^GCGCC MroI 3466 TCCGGA T^CCGGA NarI 4067 GGCGCC GG^CGCC PaeR7I 4243 CTCGAG C^TCGAG PmaCI 2486 CACGTG CAC^GTG PmlI 2486 CACGTG CAC^GTG PshAI 1913 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 4082 ATTAAT AT^TAAT Psp1406I 4121 AACGTT AA^CGTT RcaI 4209 TCATGA T^CATGA SalI 3561 GTCGAC G^TCGAC SapI 4484 GCTCTTC GCTCTTC(1/4) SbfI 2845 CCTGCAGG CCTGCA^GG ScaI 3538 AGTACT AGT^ACT SexAI 841 ACCTGGT A^CC[AT]GGT SfiI 1729 GGCCCCGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 4067 GGCGCC GGC^GCC Sse8387I 2845 CCTGCAGG CCTGCA^GG SspI 5212 AATATT AAT^ATT XhoI 4243 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AflII 1484 CTTAAG C^TTAAG 1610 CTTAAG AflIII 535 ACATGT A^C[GA][CT]GT 4202 ACATGT ApaI 3660 GGGCCC GGGCC^C 4730 GGGCCC Asp700I 3038 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5211 GAATATTTTC BbvCI 5131 CCTCAGC CCTCAGC(-5/-2) 3317 GCTGAGG BcgI 4245 CGAGGGTGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 4675 CGAGGTGTCTGC BfrI 1484 CTTAAG C^TTAAG 1610 CTTAAG BlpI 812 GCTCAGC GC^T[ACGT]AGC 3326 GCTCAGC Bpu1102I 812 GCTCAGC GC^T[ACGT]AGC 3326 GCTCAGC BsaAI 2486 CACGTG [CT]AC^GT[GA] 2643 TACGTG BsaI 4595 GAGACC GGTCTC(1/5) 212 GGTCTC BsmBI 2127 CGTCTC CGTCTC(1/5) 4602 CGTCTC BsmI 4216 GAATGC GAATGC(1/-1) 4572 GCATTC Bsp1407I 5249 TGTACA T^GTACA 5527 TGTACA BspLU11I 535 ACATGT A^CATGT 4202 ACATGT BsrGI 5249 TGTACA T^GTACA 5527 TGTACA BstAPI 2388 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4822 GCAGTATATGC BstXI 2998 CCATCGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3025 CCATCTCACTGG CelII 812 GCTCAGC GC^T[ACGT]AGC 3326 GCTCAGC DraIII 1803 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG 5187 CACTGTGTG EcoT22I 4930 ATGCAT ATGCA^T 5309 ATGCAT HgaI 4305 GCGTC GACGC(5/10) 4799 GCGTC HincII 3561 GTCGAC GT[CT]^[GA]AC 4088 GTCAAC HindII 3561 GTCGAC GT[CT]^[GA]AC 4088 GTCAAC HindIII 174 AAGCTT A^AGCTT 3642 AAGCTT NsiI 4930 ATGCAT ATGCA^T 5309 ATGCAT SspBI 5249 TGTACA T^GTACA 5527 TGTACA XmnI 3038 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5211 GAATATTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 130 enzymes) # ######################################### AccI AccII AciI AfaI AluI AlwI AlwNI AocI AspHI AspI AvaI AvaII AvrII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BlnI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BssSI BstEII BstF5I BstNI BstPI BstUI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NheI NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII XmaCI XmaI