##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
Acc65I AccIII AclI AflII AscI
Asp718I AviII AvrII BamHI BbeI
BcgI BfrI BglII BlnI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BspHI BssHII Bst1107I BstBI BstEII
BstPI BstYI BstZ17I ClaI CpoI
EagI EclXI Eco52I EcoO65I EcoRI
EcoT22I FseI FspI HindIII HpaI
KasI KpnI KspI MfeI MflI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PmeI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SwaI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccI 1916 GTCTAC GT^[AC][GT]AC
AcsI 1517 GAATTT [GA]^AATT[CT]
AflIII 2980 ACATGT A^C[GA][CT]GT
AgeI 3553 ACCGGT A^CCGGT
AhdI 5094 GACAAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 5070 AGCGCT AGC^GCT
ApoI 1517 GAATTT [GA]^AATT[CT]
AspEI 5094 GACAAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 110 CTCGAG C^[CT]CG[GA]G
BbrPI 3465 CACGTG CAC^GTG
BsoBI 110 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1558 TGTACA T^GTACA
BspLU11I 2980 ACATGT A^CATGT
BsrBI 5253 CCGCTC CCGCTC(-3/-3)
BsrGI 1558 TGTACA T^GTACA
DraIII 4485 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 5094 GACAAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 3510 GAAGAG CTCTTC(1/4)
Eco47III 5070 AGCGCT AGC^GCT
EcoRV 3285 GATATC GAT^ATC
HincII 3593 GTCAAC GT[CT]^[GA]AC
HindII 3593 GTCAAC GT[CT]^[GA]AC
Ksp632I 3510 GAAGAG CTCTTC(1/4)
PaeR7I 110 CTCGAG C^TCGAG
PinAI 3553 ACCGGT A^CCGGT
PmaCI 3465 CACGTG CAC^GTG
PmlI 3465 CACGTG CAC^GTG
PpuMI 132 AGGACCT [GA]G^G[AT]CC[CT]
SacI 4506 GAGCTC GAGCT^C
ScaI 2098 AGTACT AGT^ACT
SspBI 1558 TGTACA T^GTACA
SspI 1182 AATATT AAT^ATT
StuI 2247 AGGCCT AGG^CCT
TfiI 16 GAATC G^A[AT]TC
XbaI 2758 TCTAGA T^CTAGA
XhoI 110 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AocI 3902 CCTCAGG CC^T[ACGT]AGG
5605 CCTCAGG
ApaI 797 GGGCCC GGGCC^C
2144 GGGCCC
AseI 1680 ATTAAT AT^TAAT
1694 ATTAAT
AsnI 1680 ATTAAT AT^TAAT
1694 ATTAAT
Asp700I 445 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5484 GAACAGTTTC
BbvCI 1350 CCTCAGC CCTCAGC(-5/-2)
4205 GCTGAGG
BsaAI 1758 CACGTA [CT]AC^GT[GA]
3465 CACGTG
BsaBI 4319 GATCGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4706 GATACGCATC
BsmI 1902 GAATGC GAATGC(1/-1)
4675 GCATTC
BssSI 250 CACGAG CACGAG(-5/-1)
4260 CACGAG
Bsu36I 3902 CCTCAGG CC^T[ACGT]AGG
5605 CCTCAGG
Eco81I 3902 CCTCAGG CC^T[ACGT]AGG
5605 CCTCAGG
MamI 4319 GATCGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4706 GATACGCATC
NheI 4671 GCTAGC G^CTAGC
5229 GCTAGC
NspI 2014 ACATGC [GA]CATG^[CT]
2980 ACATGT
PshBI 1680 ATTAAT AT^TAAT
1694 ATTAAT
XmnI 445 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5484 GAACAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaLI
AspHI AspI AvaII BalI BanI
BanII BbsI BbvI BciVI BclI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DrdI
DsaI EaeI EcoNI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PflFI PflMI PleI PshAI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI