################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## Acc65I AccIII AclI AflII AscI Asp718I AviII AvrII BamHI BbeI BcgI BfrI BglII BlnI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspHI BssHII Bst1107I BstBI BstEII BstPI BstYI BstZ17I ClaI CpoI EagI EclXI Eco52I EcoO65I EcoRI EcoT22I FseI FspI HindIII HpaI KasI KpnI KspI MfeI MflI MluI MroI MunI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PmeI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccI 1916 GTCTAC GT^[AC][GT]AC AcsI 1517 GAATTT [GA]^AATT[CT] AflIII 2980 ACATGT A^C[GA][CT]GT AgeI 3553 ACCGGT A^CCGGT AhdI 5094 GACAAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 5070 AGCGCT AGC^GCT ApoI 1517 GAATTT [GA]^AATT[CT] AspEI 5094 GACAAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 110 CTCGAG C^[CT]CG[GA]G BbrPI 3465 CACGTG CAC^GTG BsoBI 110 CTCGAG C^[CT]CG[GA]G Bsp1407I 1558 TGTACA T^GTACA BspLU11I 2980 ACATGT A^CATGT BsrBI 5253 CCGCTC CCGCTC(-3/-3) BsrGI 1558 TGTACA T^GTACA DraIII 4485 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 5094 GACAAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 3510 GAAGAG CTCTTC(1/4) Eco47III 5070 AGCGCT AGC^GCT EcoRV 3285 GATATC GAT^ATC HincII 3593 GTCAAC GT[CT]^[GA]AC HindII 3593 GTCAAC GT[CT]^[GA]AC Ksp632I 3510 GAAGAG CTCTTC(1/4) PaeR7I 110 CTCGAG C^TCGAG PinAI 3553 ACCGGT A^CCGGT PmaCI 3465 CACGTG CAC^GTG PmlI 3465 CACGTG CAC^GTG PpuMI 132 AGGACCT [GA]G^G[AT]CC[CT] SacI 4506 GAGCTC GAGCT^C ScaI 2098 AGTACT AGT^ACT SspBI 1558 TGTACA T^GTACA SspI 1182 AATATT AAT^ATT StuI 2247 AGGCCT AGG^CCT TfiI 16 GAATC G^A[AT]TC XbaI 2758 TCTAGA T^CTAGA XhoI 110 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AocI 3902 CCTCAGG CC^T[ACGT]AGG 5605 CCTCAGG ApaI 797 GGGCCC GGGCC^C 2144 GGGCCC AseI 1680 ATTAAT AT^TAAT 1694 ATTAAT AsnI 1680 ATTAAT AT^TAAT 1694 ATTAAT Asp700I 445 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5484 GAACAGTTTC BbvCI 1350 CCTCAGC CCTCAGC(-5/-2) 4205 GCTGAGG BsaAI 1758 CACGTA [CT]AC^GT[GA] 3465 CACGTG BsaBI 4319 GATCGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4706 GATACGCATC BsmI 1902 GAATGC GAATGC(1/-1) 4675 GCATTC BssSI 250 CACGAG CACGAG(-5/-1) 4260 CACGAG Bsu36I 3902 CCTCAGG CC^T[ACGT]AGG 5605 CCTCAGG Eco81I 3902 CCTCAGG CC^T[ACGT]AGG 5605 CCTCAGG MamI 4319 GATCGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4706 GATACGCATC NheI 4671 GCTAGC G^CTAGC 5229 GCTAGC NspI 2014 ACATGC [GA]CATG^[CT] 2980 ACATGT PshBI 1680 ATTAAT AT^TAAT 1694 ATTAAT XmnI 445 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5484 GAACAGTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 126 enzymes) # ######################################### AatII AccII AciI AcyI AfaI AluI Alw44I AlwI AlwNI ApaLI AspHI AspI AvaII BalI BanI BanII BbsI BbvI BciVI BclI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DrdI DsaI EaeI EcoNI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PflFI PflMI PleI PshAI PstI PvuII RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI