##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI AviII BbeI BbrPI
BcgI BglII BlpI Bpu1102I BsaHI
BsaWI BseAI BsiWI BsmBI BspDI
BspEI BsrBI BssHII BssSI BstBI
BstUI BstXI CelII ClaI CpoI
DraIII Eco47III EcoNI FspI HaeII
Hin1I HpaI KasI KspI MluI
MroI MvnI NarI NcoI NheI
NotI NruI NspV PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SfoI SfuI SgrAI
SnaBI Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AflII 6097 CTTAAG C^TTAAG
AflIII 5205 ACATGT A^C[GA][CT]GT
AhdI 544 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 5102 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 544 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4023 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 5352 CCTAGG C^CTAGG
BbvCI 5463 CCTCAGC CCTCAGC(-5/-2)
BclI 5417 TGATCA T^GATCA
BfrI 6097 CTTAAG C^TTAAG
BlnI 5352 CCTAGG C^CTAGG
BsaAI 1059 TACGTG [CT]AC^GT[GA]
BsaBI 3104 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 4603 CTCCTC GAGGAG(10/8)
BsiEI 4175 CGGCCG CG[GA][CT]^CG
BspHI 5034 TCATGA T^CATGA
BspLU11I 5205 ACATGT A^CATGT
BspMI 2894 GCAGGT ACCTGC(4/8)
BstAPI 308 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EagI 4175 CGGCCG C^GGCCG
Eam1105I 544 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 4175 CGGCCG C^GGCCG
Eco52I 4175 CGGCCG C^GGCCG
EcoT22I 5039 ATGCAT ATGCA^T
FbaI 5417 TGATCA T^GATCA
FseI 4172 GGCCGGCC GGCCGG^CC
MamI 3104 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NsiI 5039 ATGCAT ATGCA^T
PacI 6437 TTAATTAA TTAAT^TAA
PflFI 4023 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3797 CCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 4511 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 5034 TCATGA T^CATGA
SmaI 5706 CCCGGG CCC^GGG
SpeI 5436 ACTAGT A^CTAGT
SphI 4881 GCATGC GCATG^C
Tth111I 4023 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3797 CCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2440 TCTAGA T^CTAGA
XmaCI 5706 CCCGGG C^CCGGG
XmaI 5706 CCCGGG C^CCGGG
XmnI 5102 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwNI 309 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1401 CAGGGGCTG
AvaI 456 CTCGGG C^[CT]CG[GA]G
5706 CCCGGG
BsmI 561 GAATGC GAATGC(1/-1)
1827 GCATTC
BsoBI 456 CTCGGG C^[CT]CG[GA]G
5706 CCCGGG
Bsp1407I 4711 TGTACA T^GTACA
6264 TGTACA
BsrGI 4711 TGTACA T^GTACA
6264 TGTACA
Bst1107I 804 GTATAC GTA^TAC
3156 GTATAC
BstZ17I 804 GTATAC GTA^TAC
3156 GTATAC
CfoI 4501 GCGC GCG^C
5473 GCGC
DsaI 4540 CCGTGG C^C[GA][CT]GG
4732 CCACGG
EcoRV 759 GATATC GAT^ATC
1883 GATATC
FauI 204 GCGGG CCCGC(4/6)
222 GCGGG
HgaI 2598 GACGC GACGC(5/10)
4464 GACGC
HhaI 4501 GCGC GCG^C
5473 GCGC
HinP1I 4501 GCGC G^CGC
5473 GCGC
HindIII 96 AAGCTT A^AGCTT
5731 AAGCTT
MaeII 628 ACGT A^CGT
1060 ACGT
NaeI 266 GCCGGC GCC^GGC
4173 GCCGGC
NdeI 5806 CATATG CA^TATG
6105 CATATG
NgoMIV 266 GCCGGC G^CCGGC
4173 GCCGGC
SacI 1836 GAGCTC GAGCT^C
1938 GAGCTC
SspBI 4711 TGTACA T^GTACA
6264 TGTACA
SspI 5868 AATATT AAT^ATT
6349 AATATT
TaqI 4924 TCGA T^CGA
5127 TCGA
TthHB8I 4924 TCGA T^CGA
5127 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
Acc65I AccI AciI AcsI AfaI
AluI AlwI AocI ApaI ApoI
Asp718I AspHI AvaII BalI BamHI
BanI BanII BbsI BbvI BciVI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrFI BsrI BssKI BstEII
BstF5I BstNI BstPI BstYI Bsu36I
Cac8I Cfr10I Cfr13I DdeI DpnII
DraI DraII DrdI EaeI EarI
Eco81I EcoO109I EcoO65I EcoRI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HincII HindII HinfI HpaII HphI
ItaI KpnI Ksp632I MaeI MaeIII
MboI MboII MfeI MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MunI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SfiI SmlI StuI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XcmI XhoII