##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
AgeI AscI AseI AsnI BbeI
BciVI BglII BlpI Bpu1102I BsiWI
BspDI BspHI BspLU11I Bst1107I BstEII
BstPI BstZ17I CelII ClaI CpoI
DrdI EcoO65I EcoRV FseI KasI
MluI NarI NotI NruI PacI
PinAI PmeI PshAI PshBI PvuI
RcaI RsrII SalI SbfI ScaI
SfoI SgrAI SnaBI Sse8387I SspI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 10 GGTACC G^GTACC
AccI 5761 GTAGAC GT^[AC][GT]AC
AclI 5854 AACGTT AA^CGTT
AflII 3648 CTTAAG C^TTAAG
Aor51HI 2415 AGCGCT AGC^GCT
Asp718I 10 GGTACC G^GTACC
AspI 3042 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 5621 CCTAGG C^CTAGG
BamHI 1700 GGATCC G^GATCC
BcgI 3875 GCATTCCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3459 TGATCA T^GATCA
BfrI 3648 CTTAAG C^TTAAG
BlnI 5621 CCTAGG C^CTAGG
BsaBI 3787 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 831 CGGCCG CG[GA][CT]^CG
Bsp1407I 272 TGTACA T^GTACA
BsrDI 71 CATTGC GCAATG(2/0)
BsrGI 272 TGTACA T^GTACA
BssHII 4793 GCGCGC G^CGCGC
DraI 2466 TTTAAA TTT^AAA
DraIII 2939 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 831 CGGCCG C^GGCCG
EclXI 831 CGGCCG C^GGCCG
Eco47III 2415 AGCGCT AGC^GCT
Eco52I 831 CGGCCG C^GGCCG
EcoRI 4465 GAATTC G^AATTC
EcoT22I 4017 ATGCAT ATGCA^T
FbaI 3459 TGATCA T^GATCA
HindIII 99 AAGCTT A^AGCTT
HpaI 4086 GTTAAC GTT^AAC
KpnI 10 GGTACC GGTAC^C
MamI 3787 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 5097 GCCGGC GCC^GGC
NgoMIV 5097 GCCGGC G^CCGGC
NheI 1945 GCTAGC G^CTAGC
NsiI 4017 ATGCAT ATGCA^T
PaeR7I 2145 CTCGAG C^TCGAG
PflFI 3042 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 5854 AACGTT AA^CGTT
SapI 3099 GCTCTTC GCTCTTC(1/4)
SfiI 3368 GGCCCTAAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 4052 ACTAGT A^CTAGT
SphI 1495 GCATGC GCATG^C
SspBI 272 TGTACA T^GTACA
Tth111I 3042 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2145 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccIII 656 TCCGGA T^CCGGA
1820 TCCGGA
Asp700I 187 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
660 GAATCTGTTC
BbvCI 126 CCTCAGC CCTCAGC(-5/-2)
4273 CCTCAGC
BseAI 656 TCCGGA T^CCGGA
1820 TCCGGA
BsmI 5032 GAATGC GAATGC(1/-1)
3875 GCATTC
BspEI 656 TCCGGA T^CCGGA
1820 TCCGGA
BstBI 3883 TTCGAA TT^CGAA
5479 TTCGAA
EcoNI 3772 CCTGGTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4261 CCTTTTCCAGG
MfeI 2999 CAATTG C^AATTG
4865 CAATTG
MroI 656 TCCGGA T^CCGGA
1820 TCCGGA
MunI 2999 CAATTG C^AATTG
4865 CAATTG
NdeI 1695 CATATG CA^TATG
2699 CATATG
NspV 3883 TTCGAA TT^CGAA
5479 TTCGAA
PstI 76 CTGCAG CTGCA^G
1610 CTGCAG
SacI 1045 GAGCTC GAGCT^C
4097 GAGCTC
SexAI 266 ACCTGGT A^CC[AT]GGT
3771 ACCTGGT
SfuI 3883 TTCGAA TT^CGAA
5479 TTCGAA
XmnI 187 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
660 GAATCTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 147 enzymes) #
#########################################
AatII AccII AciI AcsI AcyI
AfaI AflIII AhdI AluI Alw44I
AlwI AlwNI AocI ApaI ApaLI
ApoI AspEI AspHI AvaI AvaII
AviII BalI BanI BanII BbrPI
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
Eam1105I EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PmaCI
PmlI PpuMI PvuII RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI