##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AccII AccIII Aor51HI
ApaI AscI Asp718I AviII AvrII
BglI BglII BlnI BlpI Bpu1102I
BsaI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BsrBI BssHII Bst1107I
BstEII BstPI BstUI BstZ17I CelII
ClaI CpoI DrdI EagI EclXI
Eco47III Eco52I EcoO65I FauI FseI
FspI HindIII HpaI KpnI KspI
MfeI MluI MroI MunI MvnI
NaeI NgoMIV NheI NotI NruI
PacI PmeI PvuI RsrII SacII
SalI SbfI SexAI SfiI SmaI
SnaBI SphI Sse8387I XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 71 enzymes) #
###################################
AclI 2515 AACGTT AA^CGTT
AcyI 4174 GGCGCC G[GA]^CG[CT]C
AgeI 2409 ACCGGT A^CCGGT
AhdI 779 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1106 GTGCAC G^TGCAC
ApaLI 1106 GTGCAC G^TGCAC
AspEI 779 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4041 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 4154 CTCGAG C^[CT]CG[GA]G
BalI 4237 TGGCCA TGG^CCA
BamHI 666 GGATCC G^GATCC
BanI 4174 GGCGCC G^G[CT][GA]CC
BanII 1766 GAGCTC G[GA]GC[CT]^C
BbeI 4174 GGCGCC GGCGC^C
BbrPI 2120 CACGTG CAC^GTG
BbvCI 4145 CCTCAGC CCTCAGC(-5/-2)
BcgI 1426 CGAACACCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 4190 CCCGG CC^[GC]GG
BsaAI 2120 CACGTG [CT]AC^GT[GA]
BsaHI 4174 GGCGCC G[GA]^CG[CT]C
BseRI 1070 GAGGAG GAGGAG(10/8)
BsgI 1940 GTGCAG GTGCAG(16/14)
BsmI 1547 GAATGC GAATGC(1/-1)
BsoBI 4154 CTCGAG C^[CT]CG[GA]G
BspHI 553 TCATGA T^CATGA
BsrFI 2409 ACCGGT [GA]^CCGG[CT]
BstBI 2008 TTCGAA TT^CGAA
BstXI 4288 CCATAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 4175 GCGC GCG^C
Cfr10I 2409 ACCGGT [GA]^CCGG[CT]
DraII 4433 GGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 2301 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 4237 TGGCCA [CT]^GGCC[GA]
Eam1105I 779 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 5144 CCTTTAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 4433 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 2677 GAATTC G^AATTC
EcoRV 320 GATATC GAT^ATC
HaeII 4174 GGCGCC [GA]GCGC^[CT]
HgaI 3054 GCGTC GACGC(5/10)
HhaI 4175 GCGC GCG^C
Hin1I 4174 GGCGCC G[GA]^CG[CT]C
HinP1I 4175 GCGC G^CGC
KasI 4174 GGCGCC G^GCGCC
MluNI 4237 TGGCCA TGG^CCA
MscI 4237 TGGCCA TGG^CCA
NarI 4174 GGCGCC GG^CGCC
NciI 4190 CCCGG CC^[GC]GG
NdeI 2291 CATATG CA^TATG
NspV 2008 TTCGAA TT^CGAA
PaeR7I 4154 CTCGAG C^TCGAG
PflFI 4041 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1690 CCACCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2409 ACCGGT A^CCGGT
PmaCI 2120 CACGTG CAC^GTG
PmlI 2120 CACGTG CAC^GTG
PpuMI 4433 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 1904 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2515 AACGTT AA^CGTT
PvuII 1665 CAGCTG CAG^CTG
RcaI 553 TCATGA T^CATGA
SacI 1766 GAGCTC GAGCT^C
SapI 3522 GCTCTTC GCTCTTC(1/4)
SfoI 4174 GGCGCC GGC^GCC
SfuI 2008 TTCGAA TT^CGAA
SgrAI 2408 CACCGGTG C[GA]^CCGG[CT]G
SpeI 3571 ACTAGT A^CTAGT
Tth111I 4041 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1690 CCACCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 963 CCAGGGAAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 4154 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 887 GTAGAC GT^[AC][GT]AC
2157 GTAGAC
AflII 2841 CTTAAG C^TTAAG
4036 CTTAAG
AocI 824 CCTGAGG CC^T[ACGT]AGG
2358 CCTCAGG
AseI 4974 ATTAAT AT^TAAT
4995 ATTAAT
AsnI 4974 ATTAAT AT^TAAT
4995 ATTAAT
BciVI 1927 GGATAC GTATCC(6/5)
3710 GTATCC
BfrI 2841 CTTAAG C^TTAAG
4036 CTTAAG
BsaBI 161 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1334 GATGGACATC
BsaWI 1200 ACCGGA [AT]^CCGG[AT]
2409 ACCGGT
BspMI 4273 ACCTGC ACCTGC(4/8)
4701 GCAGGT
BssSI 1109 CACGAG CACGAG(-5/-1)
4263 CTCGTG
Bsu36I 824 CCTGAGG CC^T[ACGT]AGG
2358 CCTCAGG
DsaI 81 CCATGG C^C[GA][CT]GG
774 CCATGG
Eco81I 824 CCTGAGG CC^T[ACGT]AGG
2358 CCTCAGG
EcoT22I 3494 ATGCAT ATGCA^T
4461 ATGCAT
HincII 2633 GTTGAC GT[CT]^[GA]AC
2881 GTTGAC
HindII 2633 GTTGAC GT[CT]^[GA]AC
2881 GTTGAC
MamI 161 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1334 GATGGACATC
NcoI 81 CCATGG C^CATGG
774 CCATGG
NsiI 3494 ATGCAT ATGCA^T
4461 ATGCAT
PshBI 4974 ATTAAT AT^TAAT
4995 ATTAAT
StuI 2099 AGGCCT AGG^CCT
3962 AGGCCT
SwaI 1017 ATTTAAAT ATTT^AAAT
2790 ATTTAAAT
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BbsI BbvI BclI BfaI
BmyI BpmI BpuAI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspLU11I BsrDI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
EarI EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PstI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI