##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AccI AclI AflII AgeI AhdI
Alw44I ApaLI AscI AseI AsnI
AspEI AspI BbrPI BclI BfrI
BsmI BspHI BspLU11I Bst1107I BstBI
BstZ17I DraI Eam1105I EcoRI EcoRV
EcoT22I FbaI FseI KspI MfeI
MluI MunI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI SacII
SalI ScaI SfuI SgrAI SpeI
SphI SspI SwaI Tth111I XbaI
XcmI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 2962 GACGTC GACGT^C
Acc65I 4800 GGTACC G^GTACC
AcsI 2863 AAATTT [GA]^AATT[CT]
AocI 5768 CCTGAGG CC^T[ACGT]AGG
ApoI 2863 AAATTT [GA]^AATT[CT]
Asp700I 1518 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4800 GGTACC G^GTACC
AviII 253 TGCGCA TGC^GCA
AvrII 4023 CCTAGG C^CTAGG
BcgI 4744 CGAACCGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3140 GGATAC GTATCC(6/5)
BfaI 4024 CTAG C^TAG
BglII 1909 AGATCT A^GATCT
BlnI 4023 CCTAGG C^CTAGG
BspDI 5055 ATCGAT AT^CGAT
BsrDI 4919 CATTGC GCAATG(2/0)
BstEII 5774 GGTCACC G^GT[ACGT]ACC
BstPI 5774 GGTCACC G^GT[ACGT]ACC
Bsu36I 5768 CCTGAGG CC^T[ACGT]AGG
ClaI 5055 ATCGAT AT^CGAT
CpoI 4767 CGGTCCG CG^G[AT]CCG
EagI 796 CGGCCG C^GGCCG
EclXI 796 CGGCCG C^GGCCG
Eco52I 796 CGGCCG C^GGCCG
Eco81I 5768 CCTGAGG CC^T[ACGT]AGG
EcoO65I 5774 GGTCACC G^GT[ACGT]ACC
FspI 253 TGCGCA TGC^GCA
HindIII 899 AAGCTT A^AGCTT
HpaI 2216 GTTAAC GTT^AAC
KpnI 4800 GGTACC GGTAC^C
MaeI 4024 CTAG C^TAG
NcoI 4909 CCATGG C^CATGG
NdeI 5858 CATATG CA^TATG
RsrII 4767 CGGTCCG CG^G[AT]CCG
SbfI 3510 CCTGCAGG CCTGCA^GG
SexAI 1475 ACCTGGT A^CC[AT]GGT
SfiI 3926 GGCCGACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 4845 CTCAAG C^T[CT][GA]AG
SnaBI 4493 TACGTA TAC^GTA
Sse8387I 3510 CCTGCAGG CCTGCA^GG
XmnI 1518 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccIII 4324 TCCGGA T^CCGGA
4852 TCCGGA
AflIII 976 ACACGT A^C[GA][CT]GT
4693 ACGTGT
BamHI 230 GGATCC G^GATCC
4885 GGATCC
BsaWI 4324 TCCGGA [AT]^CCGG[AT]
4852 TCCGGA
BseAI 4324 TCCGGA T^CCGGA
4852 TCCGGA
BsiEI 796 CGGCCG CG[GA][CT]^CG
1922 CGACCG
BsiWI 546 CGTACG C^GTACG
4495 CGTACG
Bsp1407I 5570 TGTACA T^GTACA
5854 TGTACA
BspEI 4324 TCCGGA T^CCGGA
4852 TCCGGA
BsrGI 5570 TGTACA T^GTACA
5854 TGTACA
BssHII 2 GCGCGC G^CGCGC
4 GCGCGC
BssSI 4890 CACGAG CACGAG(-5/-1)
2745 CTCGTG
DraIII 4408 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
5063 CACCAAGTG
EcoNI 1476 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3872 CCTCACCGAGG
HincII 303 GTCAAC GT[CT]^[GA]AC
2216 GTTAAC
HindII 303 GTCAAC GT[CT]^[GA]AC
2216 GTTAAC
MroI 4324 TCCGGA T^CCGGA
4852 TCCGGA
NaeI 1411 GCCGGC GCC^GGC
5563 GCCGGC
NgoMIV 1411 GCCGGC G^CCGGC
5563 GCCGGC
NspI 4981 ACATGC [GA]CATG^[CT]
5377 ACATGC
PflMI 1470 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3564 CCACCTGCTGG
PpuMI 4159 AGGTCCT [GA]G^G[AT]CC[CT]
5831 GGGTCCC
SacI 2148 GAGCTC GAGCT^C
5333 GAGCTC
SspBI 5570 TGTACA T^GTACA
5854 TGTACA
Van91I 1470 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3564 CCACCTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI Aor51HI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbsI BbvCI BbvI BcnI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DrdI DsaI EaeI EarI Eco47III
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeII
MaeIII MamI MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
PleI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmaCI XmaI