##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
Acc65I AclI AflII Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AvrII BamHI BclI BfrI BlnI
BsaBI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrGI Bst1107I BstBI
BstZ17I ClaI DraI Eco47III EcoRV
EcoT22I FbaI HindIII HpaI KpnI
MamI MluI NdeI NheI NotI
NruI NsiI NspV PacI PmeI
PshAI PshBI Psp1406I PvuI RcaI
ScaI SfuI SgrAI SpeI SspBI
SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AatII 4755 GACGTC GACGT^C
AccI 1848 GTCGAC GT^[AC][GT]AC
AccIII 2140 TCCGGA T^CCGGA
AgeI 1021 ACCGGT A^CCGGT
Asp700I 1502 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 5722 TGCGCA TGC^GCA
BcgI 1795 CGAGGCACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2586 GGATAC GTATCC(6/5)
BseAI 2140 TCCGGA T^CCGGA
BspEI 2140 TCCGGA T^CCGGA
BssHII 2855 GCGCGC G^CGCGC
BstXI 4489 CCATGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 5287 CGGACCG CG^G[AT]CCG
EcoRI 3170 GAATTC G^AATTC
FspI 5722 TGCGCA TGC^GCA
HincII 1848 GTCGAC GT[CT]^[GA]AC
HindII 1848 GTCGAC GT[CT]^[GA]AC
MfeI 2874 CAATTG C^AATTG
MroI 2140 TCCGGA T^CCGGA
MseI 5743 TTAA T^TAA
MunI 2874 CAATTG C^AATTG
NspI 568 GCATGC [GA]CATG^[CT]
PaeR7I 1002 CTCGAG C^TCGAG
PinAI 1021 ACCGGT A^CCGGT
RsrII 5287 CGGACCG CG^G[AT]CCG
SalI 1848 GTCGAC G^TCGAC
SexAI 2111 ACCAGGT A^CC[AT]GGT
SfiI 3872 GGCCCTGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI 6623 TACGTA TAC^GTA
SphI 568 GCATGC GCATG^C
Tru9I 5743 TTAA T^TAA
XhoI 1002 CTCGAG C^TCGAG
XmnI 1502 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflIII 421 ACACGT A^C[GA][CT]GT
535 ACGTGT
AhdI 3567 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3923 GACTGCACGTC
AocI 2636 CCTGAGG CC^T[ACGT]AGG
6096 CCTGAGG
AspEI 3567 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3923 GACTGCACGTC
AspI 29 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1899 GACAACGTC
BstEII 2546 GGTCACC G^GT[ACGT]ACC
5349 GGTAACC
BstPI 2546 GGTCACC G^GT[ACGT]ACC
5349 GGTAACC
Bsu36I 2636 CCTGAGG CC^T[ACGT]AGG
6096 CCTGAGG
DraIII 519 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
2110 CACCAGGTG
Eam1105I 3567 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3923 GACTGCACGTC
Eco81I 2636 CCTGAGG CC^T[ACGT]AGG
6096 CCTGAGG
EcoO65I 2546 GGTCACC G^GT[ACGT]ACC
5349 GGTAACC
FseI 1669 GGCCGGCC GGCCGG^CC
5882 GGCCGGCC
PflFI 29 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1899 GACAACGTC
SapI 5418 GAAGAGC GCTCTTC(1/4)
1553 GCTCTTC
SbfI 4928 CCTGCAGG CCTGCA^GG
5480 CCTGCAGG
Sse8387I 4928 CCTGCAGG CCTGCA^GG
5480 CCTGCAGG
Tth111I 29 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1899 GACAACGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 149 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbrPI BbsI BbvCI
BbvI BcnI BfaI BglI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DrdI DsaI EaeI EagI
EarI EclXI Eco52I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeII NgoMIV
NlaIII NlaIV PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
SacI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
StuI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI