##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AclI AflII AgeI AhdI AscI
AseI AsnI AspEI BamHI BciVI
BclI BfrI BsaBI BsiWI BspDI
BspLU11I BssHII Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI Eam1105I
EcoO65I EcoRV EcoT22I FbaI FseI
HindIII HpaI MamI MfeI MunI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I RsrII SalI ScaI SexAI
SfuI SgrAI SmaI SnaBI SwaI
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 2091 GACGTC GACGT^C
AccI 4291 GTAGAC GT^[AC][GT]AC
Alw44I 717 GTGCAC G^TGCAC
ApaLI 717 GTGCAC G^TGCAC
Asp700I 303 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1508 TGCGCA TGC^GCA
AvrII 3023 CCTAGG C^CTAGG
BbeI 219 GGCGCC GGCGC^C
BbrPI 4408 CACGTG CAC^GTG
BlnI 3023 CCTAGG C^CTAGG
BsmBI 4730 GAGACG CGTCTC(1/5)
Bsp1407I 5513 TGTACA T^GTACA
BsrFI 3349 GCCGGC [GA]^CCGG[CT]
BsrGI 5513 TGTACA T^GTACA
BssSI 4397 CACGAG CACGAG(-5/-1)
Cfr10I 3349 GCCGGC [GA]^CCGG[CT]
DrdI 2085 GACACTGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 1508 TGCGCA TGC^GCA
KasI 219 GGCGCC G^GCGCC
KspI 188 CCGCGG CCGC^GG
MluI 4 ACGCGT A^CGCGT
NaeI 3349 GCCGGC GCC^GGC
NarI 219 GGCGCC GG^CGCC
NcoI 1918 CCATGG C^CATGG
NgoMIV 3349 GCCGGC G^CCGGC
PmaCI 4408 CACGTG CAC^GTG
PmlI 4408 CACGTG CAC^GTG
PshAI 5029 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 873 CGATCG CGAT^CG
SacI 2304 GAGCTC GAGCT^C
SacII 188 CCGCGG CCGC^GG
SbfI 2390 CCTGCAGG CCTGCA^GG
SfoI 219 GGCGCC GGC^GCC
SpeI 4011 ACTAGT A^CTAGT
Sse8387I 2390 CCTGCAGG CCTGCA^GG
SspBI 5513 TGTACA T^GTACA
XmnI 303 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccIII 2531 TCCGGA T^CCGGA
3133 TCCGGA
AcyI 219 GGCGCC G[GA]^CG[CT]C
2091 GACGTC
AflIII 4 ACGCGT A^C[GA][CT]GT
4407 ACACGT
BalI 998 TGGCCA TGG^CCA
1397 TGGCCA
BglII 2447 AGATCT A^GATCT
3365 AGATCT
BlpI 2301 GCTGAGC GC^T[ACGT]AGC
3929 GCTGAGC
Bpu1102I 2301 GCTGAGC GC^T[ACGT]AGC
3929 GCTGAGC
BsaHI 219 GGCGCC G[GA]^CG[CT]C
2091 GACGTC
BseAI 2531 TCCGGA T^CCGGA
3133 TCCGGA
BsmI 1201 GAATGC GAATGC(1/-1)
258 GCATTC
BspEI 2531 TCCGGA T^CCGGA
3133 TCCGGA
BspHI 947 TCATGA T^CATGA
5389 TCATGA
BsrDI 3113 CATTGC GCAATG(2/0)
4600 GCAATG
CelII 2301 GCTGAGC GC^T[ACGT]AGC
3929 GCTGAGC
DraIII 1571 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
4930 CACTGGGTG
EagI 3351 CGGCCG C^GGCCG
3944 CGGCCG
EclXI 3351 CGGCCG C^GGCCG
3944 CGGCCG
Eco52I 3351 CGGCCG C^GGCCG
3944 CGGCCG
EcoNI 1737 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2039 CCTTCTCAAGG
EcoRI 303 GAATTC G^AATTC
2506 GAATTC
Hin1I 219 GGCGCC G[GA]^CG[CT]C
2091 GACGTC
HincII 153 GTCAAC GT[CT]^[GA]AC
1014 GTCAAC
HindII 153 GTCAAC GT[CT]^[GA]AC
1014 GTCAAC
MluNI 998 TGGCCA TGG^CCA
1397 TGGCCA
MroI 2531 TCCGGA T^CCGGA
3133 TCCGGA
MscI 998 TGGCCA TGG^CCA
1397 TGGCCA
RcaI 947 TCATGA T^CATGA
5389 TCATGA
SfiI 1155 GGCCCAAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3776 GGCCTCCGAGGCC
SphI 1684 GCATGC GCATG^C
3841 GCATGC
StuI 2477 AGGCCT AGG^CCT
3784 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
Acc65I AccII AciI AcsI AfaI
AluI AlwI AlwNI AocI Aor51HI
ApaI ApoI Asp718I AspHI AspI
AvaI AvaII BanI BanII BbsI
BbvCI BbvI BcgI BcnI BfaI
BglI BmyI BpmI BpuAI BsaAI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI Eco47III
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeI
NdeII NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII