##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
AatII AclI AflII AgeI Alw44I
ApaLI AscI AseI AsnI BciVI
BfrI BglII BsiWI BspDI BspHI
BssHII Bst1107I BstBI BstZ17I ClaI
EcoRI EcoRV EcoT22I FseI HindIII
HpaI MfeI MluI MunI NdeI
NotI NruI NsiI NspV PacI
PinAI PmeI PshBI Psp1406I PvuI
RcaI SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI Sse8387I
SwaI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AocI 3915 CCTGAGG CC^T[ACGT]AGG
AviII 607 TGCGCA TGC^GCA
AvrII 2255 CCTAGG C^CTAGG
BamHI 813 GGATCC G^GATCC
BbeI 774 GGCGCC GGCGC^C
BlnI 2255 CCTAGG C^CTAGG
BsmBI 2159 CGTCTC CGTCTC(1/5)
Bsp1407I 320 TGTACA T^GTACA
BsrGI 320 TGTACA T^GTACA
BstAPI 209 GCACACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 4032 GGTTACC G^GT[ACGT]ACC
BstPI 4032 GGTTACC G^GT[ACGT]ACC
Bsu36I 3915 CCTGAGG CC^T[ACGT]AGG
CpoI 3211 CGGACCG CG^G[AT]CCG
DraI 4794 TTTAAA TTT^AAA
Eco81I 3915 CCTGAGG CC^T[ACGT]AGG
EcoO65I 4032 GGTTACC G^GT[ACGT]ACC
FspI 607 TGCGCA TGC^GCA
KasI 774 GGCGCC G^GCGCC
KspI 3644 CCGCGG CCGC^GG
NarI 774 GGCGCC GG^CGCC
PshAI 1865 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 3211 CGGACCG CG^G[AT]CCG
SacII 3644 CCGCGG CCGC^GG
SfoI 774 GGCGCC GGC^GCC
SpeI 5523 ACTAGT A^CTAGT
SspBI 320 TGTACA T^GTACA
SspI 1962 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 1261 GGTACC G^GTACC
2615 GGTACC
AccI 963 GTCTAC GT^[AC][GT]AC
1293 GTCTAC
AcsI 5224 AAATTT [GA]^AATT[CT]
5509 AAATTC
Aor51HI 3151 AGCGCT AGC^GCT
3469 AGCGCT
ApoI 5224 AAATTT [GA]^AATT[CT]
5509 AAATTC
Asp718I 1261 GGTACC G^GTACC
2615 GGTACC
BbrPI 4493 CACGTG CAC^GTG
4837 CACGTG
BbsI 2597 GAAGAC GAAGAC(2/6)
5175 GTCTTC
BcgI 2303 CGACCCGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1417 GCATTGACTTCG
BlpI 1107 GCTCAGC GC^T[ACGT]AGC
3776 GCTCAGC
Bpu1102I 1107 GCTCAGC GC^T[ACGT]AGC
3776 GCTCAGC
BpuAI 2597 GAAGAC GAAGAC(2/6)
5175 GTCTTC
BsaBI 1343 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5409 GATATAAATC
BspLU11I 2338 ACATGT A^CATGT
2359 ACATGT
BspMI 499 ACCTGC ACCTGC(4/8)
1949 GCAGGT
BstYI 813 GGATCC [GA]^GATC[CT]
2980 AGATCC
CelII 1107 GCTCAGC GC^T[ACGT]AGC
3776 GCTCAGC
DrdI 3995 GACCTCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4396 GACCCAGGAGTC
EagI 3112 CGGCCG C^GGCCG
3641 CGGCCG
EclXI 3112 CGGCCG C^GGCCG
3641 CGGCCG
Eco47III 3151 AGCGCT AGC^GCT
3469 AGCGCT
Eco52I 3112 CGGCCG C^GGCCG
3641 CGGCCG
HincII 1323 GTCAAC GT[CT]^[GA]AC
3617 GTCAAC
HindII 1323 GTCAAC GT[CT]^[GA]AC
3617 GTCAAC
KpnI 1261 GGTACC GGTAC^C
2615 GGTACC
MamI 1343 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5409 GATATAAATC
MflI 813 GGATCC [GA]^GATC[CT]
2980 AGATCC
NcoI 1204 CCATGG C^CATGG
1645 CCATGG
NheI 3630 GCTAGC G^CTAGC
4111 GCTAGC
PaeR7I 797 CTCGAG C^TCGAG
2398 CTCGAG
PmaCI 4493 CACGTG CAC^GTG
4837 CACGTG
PmlI 4493 CACGTG CAC^GTG
4837 CACGTG
PstI 1064 CTGCAG CTGCA^G
3810 CTGCAG
PvuII 2521 CAGCTG CAG^CTG
3553 CAGCTG
SacI 135 GAGCTC GAGCT^C
4464 GAGCTC
SapI 971 GCTCTTC GCTCTTC(1/4)
1007 GCTCTTC
SphI 1945 GCATGC GCATG^C
4141 GCATGC
XbaI 4271 TCTAGA T^CTAGA
5074 TCTAGA
XhoI 797 CTCGAG C^TCGAG
2398 CTCGAG
XhoII 813 GGATCC [GA]^GATC[CT]
2980 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AflIII AhdI AluI AlwI AlwNI
ApaI Asp700I AspEI AspHI AspI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BclI BcnI BfaI
BglI BmyI BpmI BsaAI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspEI BsrBI BsrDI BsrFI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI Eam1105I EarI EcoNI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MroI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XmaCI XmaI XmnI