##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspI AviII BamHI BbeI BbrPI
BcgI BclI BglI BsaAI BsaHI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BspDI BspEI BsrBI BssHII
BssSI BstBI BstUI ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
FauI FbaI FseI FspI HaeII
Hin1I KasI KpnI KspI MluI
MroI MvnI NaeI NarI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PvuI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SwaI TaqI Tth111I TthHB8I
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AflII 2572 CTTAAG C^TTAAG
AflIII 896 ACATGT A^C[GA][CT]GT
AlwNI 4050 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 52 CCTCAGG CC^T[ACGT]AGG
AspHI 2204 GAGCAC G[AT]GC[AT]^C
AvaI 3899 CCCGGG C^[CT]CG[GA]G
BalI 347 TGGCCA TGG^CCA
BanI 807 GGTGCC G^G[CT][GA]CC
BbsI 1797 GAAGAC GAAGAC(2/6)
BciVI 2446 GGATAC GTATCC(6/5)
BfrI 2572 CTTAAG C^TTAAG
BglII 3259 AGATCT A^GATCT
BlpI 67 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 67 GCTCAGC GC^T[ACGT]AGC
BpuAI 1797 GAAGAC GAAGAC(2/6)
BsaI 1519 GAGACC GGTCTC(1/5)
BsiHKAI 2204 GAGCAC G[AT]GC[AT]^C
BsoBI 3899 CCCGGG C^[CT]CG[GA]G
BspLU11I 896 ACATGT A^CATGT
BsrFI 4889 GCCGGT [GA]^CCGG[CT]
Bsu36I 52 CCTCAGG CC^T[ACGT]AGG
CelII 67 GCTCAGC GC^T[ACGT]AGC
Cfr10I 4889 GCCGGT [GA]^CCGG[CT]
DraIII 3985 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 179 GACTTGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 347 TGGCCA [CT]^GGCC[GA]
Eco81I 52 CCTCAGG CC^T[ACGT]AGG
EcoNI 811 CCTGGAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 1638 GCGTC GACGC(5/10)
HpaI 2752 GTTAAC GTT^AAC
MfeI 5266 CAATTG C^AATTG
MluNI 347 TGGCCA TGG^CCA
MscI 347 TGGCCA TGG^CCA
MunI 5266 CAATTG C^AATTG
NcoI 685 CCATGG C^CATGG
PpuMI 1476 AGGTCCC [GA]G^G[AT]CC[CT]
SapI 3955 GCTCTTC GCTCTTC(1/4)
SmaI 3899 CCCGGG CCC^GGG
XbaI 3262 TCTAGA T^CTAGA
XcmI 3113 CCAGAGGACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 3899 CCCGGG C^CCGGG
XmaI 3899 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AclI 429 AACGTT AA^CGTT
2981 AACGTT
AvrII 1668 CCTAGG C^CTAGG
5074 CCTAGG
BanII 689 GGGCTC G[GA]GC[CT]^C
4220 GGGCTC
BcnI 3899 CCCGG CC^[GC]GG
3900 CCGGG
BlnI 1668 CCTAGG C^CTAGG
5074 CCTAGG
BspHI 6 TCATGA T^CATGA
3377 TCATGA
BstAPI 4049 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4663 GCAAACAATGC
BstEII 4501 GGTGACC G^GT[ACGT]ACC
5805 GGTTACC
BstPI 4501 GGTGACC G^GT[ACGT]ACC
5805 GGTTACC
CfoI 537 GCGC GCG^C
796 GCGC
DraII 1476 AGGTCCC [GA]G^G[ACGT]CC[CT]
1606 GGGGCCT
DsaI 685 CCATGG C^C[GA][CT]GG
1709 CCACGG
EarI 3956 CTCTTC CTCTTC(1/4)
5887 GAAGAG
EcoO109I 1476 AGGTCCC [GA]G^G[ACGT]CC[CT]
1606 GGGGCCT
EcoO65I 4501 GGTGACC G^GT[ACGT]ACC
5805 GGTTACC
EcoRV 1060 GATATC GAT^ATC
4191 GATATC
HapII 3900 CCGG C^CGG
4890 CCGG
HhaI 537 GCGC GCG^C
796 GCGC
HinP1I 537 GCGC G^CGC
796 GCGC
HindIII 1580 AAGCTT A^AGCTT
1834 AAGCTT
HpaII 3900 CCGG C^CGG
4890 CCGG
Ksp632I 3956 CTCTTC CTCTTC(1/4)
5887 GAAGAG
MslI 1042 CACATTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3284 CACTTGTGTG
MspI 3900 CCGG C^CGG
4890 CCGG
NciI 3899 CCCGG CC^[GC]GG
3900 CCGGG
Psp1406I 429 AACGTT AA^CGTT
2981 AACGTT
PstI 2311 CTGCAG CTGCA^G
4056 CTGCAG
PvuII 83 CAGCTG CAG^CTG
3623 CAGCTG
RcaI 6 TCATGA T^CATGA
3377 TCATGA
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI AvaII
BbvCI BbvI BfaI BmyI BpmI
BsaBI BsaJI BsgI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BspMI BsrDI BsrGI BsrI BssKI
Bst1107I BstF5I BstNI BstXI BstYI
BstZ17I Cac8I Cfr13I DdeI DpnII
DraI EcoRI EcoRII EcoT14I EcoT22I
Fnu4HI FokI HaeIII HincII HindII
HinfI HphI ItaI MaeI MaeII
MaeIII MamI MboI MboII MflI
MnlI MseI MspA1I MvaI MwoI
NdeI NdeII NlaIII NlaIV NsiI
NspI PflMI PleI PshBI RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SphI SspBI SspI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XhoII