################################## # NO CUT ENZYMES ( 54 enzymes) # ################################## AatII AccI AclI AflII AgeI AhdI Alw44I ApaLI AscI AseI AsnI AspEI BcgI BfrI BsiWI Bst1107I BstBI BstEII BstPI BstZ17I CpoI DrdI Eam1105I EcoO65I EcoRV EcoT22I HincII HindII HindIII HpaI MfeI MluI MunI NdeI NheI NotI NsiI NspV PacI PinAI PmeI PshBI Psp1406I RsrII SalI SapI ScaI SfuI SnaBI SpeI SphI SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 506 GGTACC G^GTACC AccIII 2026 TCCGGA T^CCGGA Aor51HI 3096 AGCGCT AGC^GCT Asp700I 2642 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 506 GGTACC G^GTACC AviII 3075 TGCGCA TGC^GCA BamHI 936 GGATCC G^GATCC BbsI 4704 GTCTTC GAAGAC(2/6) BciVI 405 GGATAC GTATCC(6/5) BglII 884 AGATCT A^GATCT BlpI 3373 GCTGAGC GC^T[ACGT]AGC Bpu1102I 3373 GCTGAGC GC^T[ACGT]AGC BpuAI 4704 GTCTTC GAAGAC(2/6) BsaBI 2162 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 196 GGTCTC GGTCTC(1/5) BseAI 2026 TCCGGA T^CCGGA BsmBI 1315 CGTCTC CGTCTC(1/5) Bsp1407I 1250 TGTACA T^GTACA BspDI 1000 ATCGAT AT^CGAT BspEI 2026 TCCGGA T^CCGGA BspHI 4096 TCATGA T^CATGA BsrDI 648 CATTGC GCAATG(2/0) BsrGI 1250 TGTACA T^GTACA BstAPI 3592 GCACATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 3373 GCTGAGC GC^T[ACGT]AGC ClaI 1000 ATCGAT AT^CGAT Eco47III 3096 AGCGCT AGC^GCT EcoNI 3197 CCTGTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1058 GAATTC G^AATTC FseI 3901 GGCCGGCC GGCCGG^CC FspI 3075 TGCGCA TGC^GCA KpnI 506 GGTACC GGTAC^C KspI 1845 CCGCGG CCGC^GG MamI 2162 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 2026 TCCGGA T^CCGGA NruI 2306 TCGCGA TCG^CGA PaeR7I 1070 CTCGAG C^TCGAG PshAI 1685 GACGCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 1002 CGATCG CGAT^CG RcaI 4096 TCATGA T^CATGA SacII 1845 CCGCGG CCGC^GG SbfI 4296 CCTGCAGG CCTGCA^GG SexAI 1343 ACCAGGT A^CC[AT]GGT SfiI 3901 GGCCGGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC Sse8387I 4296 CCTGCAGG CCTGCA^GG SspBI 1250 TGTACA T^GTACA XhoI 1070 CTCGAG C^TCGAG XmnI 2642 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AflIII 1119 ACATGT A^C[GA][CT]GT 4866 ACATGT AspI 4617 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 5145 GACCCTGTC AvrII 640 CCTAGG C^CTAGG 4713 CCTAGG BbrPI 1664 CACGTG CAC^GTG 3037 CACGTG BclI 806 TGATCA T^GATCA 2167 TGATCA BlnI 640 CCTAGG C^CTAGG 4713 CCTAGG BspLU11I 1119 ACATGT A^CATGT 4866 ACATGT BssHII 1222 GCGCGC G^CGCGC 2558 GCGCGC BssSI 880 CACGAG CACGAG(-5/-1) 3287 CACGAG EagI 1881 CGGCCG C^GGCCG 2018 CGGCCG EclXI 1881 CGGCCG C^GGCCG 2018 CGGCCG Eco52I 1881 CGGCCG C^GGCCG 2018 CGGCCG FbaI 806 TGATCA T^GATCA 2167 TGATCA PflFI 4617 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 5145 GACCCTGTC PmaCI 1664 CACGTG CAC^GTG 3037 CACGTG PmlI 1664 CACGTG CAC^GTG 3037 CACGTG SgrAI 1448 CACCGGCG C[GA]^CCGG[CT]G 2626 CGCCGGCG Tth111I 4617 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 5145 GACCCTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 138 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApaI ApoI AspHI AvaI AvaII BalI BanI BanII BbeI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BsaAI BsaHI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI