##################################
# NO CUT ENZYMES (   54 enzymes) #
##################################

AatII       AccI        AclI        AflII       AgeI        
AhdI        Alw44I      ApaLI       AscI        AseI        
AsnI        AspEI       BcgI        BfrI        BsiWI       
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
CpoI        DrdI        Eam1105I    EcoO65I     EcoRV       
EcoT22I     HincII      HindII      HindIII     HpaI        
MfeI        MluI        MunI        NdeI        NheI        
NotI        NsiI        NspV        PacI        PinAI       
PmeI        PshBI       Psp1406I    RsrII       SalI        
SapI        ScaI        SfuI        SnaBI       SpeI        
SphI        SspI        SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

Acc65I        506  GGTACC               G^GTACC
AccIII       2026  TCCGGA               T^CCGGA
Aor51HI      3096  AGCGCT               AGC^GCT
Asp700I      2642  GAACGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       506  GGTACC               G^GTACC
AviII        3075  TGCGCA               TGC^GCA
BamHI         936  GGATCC               G^GATCC
BbsI         4704  GTCTTC               GAAGAC(2/6)
BciVI         405  GGATAC               GTATCC(6/5)
BglII         884  AGATCT               A^GATCT
BlpI         3373  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     3373  GCTGAGC              GC^T[ACGT]AGC
BpuAI        4704  GTCTTC               GAAGAC(2/6)
BsaBI        2162  GATAATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          196  GGTCTC               GGTCTC(1/5)
BseAI        2026  TCCGGA               T^CCGGA
BsmBI        1315  CGTCTC               CGTCTC(1/5)
Bsp1407I     1250  TGTACA               T^GTACA
BspDI        1000  ATCGAT               AT^CGAT
BspEI        2026  TCCGGA               T^CCGGA
BspHI        4096  TCATGA               T^CATGA
BsrDI         648  CATTGC               GCAATG(2/0)
BsrGI        1250  TGTACA               T^GTACA
BstAPI       3592  GCACATGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII        3373  GCTGAGC              GC^T[ACGT]AGC
ClaI         1000  ATCGAT               AT^CGAT
Eco47III     3096  AGCGCT               AGC^GCT
EcoNI        3197  CCTGTGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1058  GAATTC               G^AATTC
FseI         3901  GGCCGGCC             GGCCGG^CC
FspI         3075  TGCGCA               TGC^GCA
KpnI          506  GGTACC               GGTAC^C
KspI         1845  CCGCGG               CCGC^GG
MamI         2162  GATAATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         2026  TCCGGA               T^CCGGA
NruI         2306  TCGCGA               TCG^CGA
PaeR7I       1070  CTCGAG               C^TCGAG
PshAI        1685  GACGCGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI         1002  CGATCG               CGAT^CG
RcaI         4096  TCATGA               T^CATGA
SacII        1845  CCGCGG               CCGC^GG
SbfI         4296  CCTGCAGG             CCTGCA^GG
SexAI        1343  ACCAGGT              A^CC[AT]GGT
SfiI         3901  GGCCGGCCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I     4296  CCTGCAGG             CCTGCA^GG
SspBI        1250  TGTACA               T^GTACA
XhoI         1070  CTCGAG               C^TCGAG
XmnI         2642  GAACGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AflIII       1119  ACATGT               A^C[GA][CT]GT
             4866  ACATGT              
AspI         4617  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             5145  GACCCTGTC           
AvrII         640  CCTAGG               C^CTAGG
             4713  CCTAGG              
BbrPI        1664  CACGTG               CAC^GTG
             3037  CACGTG              
BclI          806  TGATCA               T^GATCA
             2167  TGATCA              
BlnI          640  CCTAGG               C^CTAGG
             4713  CCTAGG              
BspLU11I     1119  ACATGT               A^CATGT
             4866  ACATGT              
BssHII       1222  GCGCGC               G^CGCGC
             2558  GCGCGC              
BssSI         880  CACGAG               CACGAG(-5/-1)
             3287  CACGAG              
EagI         1881  CGGCCG               C^GGCCG
             2018  CGGCCG              
EclXI        1881  CGGCCG               C^GGCCG
             2018  CGGCCG              
Eco52I       1881  CGGCCG               C^GGCCG
             2018  CGGCCG              
FbaI          806  TGATCA               T^GATCA
             2167  TGATCA              
PflFI        4617  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             5145  GACCCTGTC           
PmaCI        1664  CACGTG               CAC^GTG
             3037  CACGTG              
PmlI         1664  CACGTG               CAC^GTG
             3037  CACGTG              
SgrAI        1448  CACCGGCG             C[GA]^CCGG[CT]G
             2626  CGCCGGCG            
Tth111I      4617  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             5145  GACCCTGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  138 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       AocI        ApaI        
ApoI        AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbeI        BbvCI       BbvI        
BcnI        BfaI        BglI        BmyI        BpmI        
BsaAI       BsaHI       BsaJI       BsaWI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        BsoBI       Bsp1286I    
BspMI       BsrBI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstXI       BstYI       
Bsu36I      Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraI        DraII       DraIII      
DsaI        EaeI        EarI        Eco81I      EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        KasI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NarI        NciI        NcoI        NdeII       NgoMIV      
NlaIII      NlaIV       NspI        PflMI       PleI        
PpuMI       PstI        PvuII       RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SfoI        SmaI        SmlI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XcmI        
XhoII       XmaCI       XmaI