##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII AccI AclI AflII AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI AspEI BcgI BfrI BsiWI
Bst1107I BstBI BstEII BstPI BstZ17I
CpoI DrdI Eam1105I EcoO65I EcoRV
EcoT22I HincII HindII HindIII HpaI
MfeI MluI MunI NdeI NheI
NotI NsiI NspV PacI PinAI
PmeI PshBI Psp1406I RsrII SalI
SapI ScaI SfuI SnaBI SpeI
SphI SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 506 GGTACC G^GTACC
AccIII 2026 TCCGGA T^CCGGA
Aor51HI 3096 AGCGCT AGC^GCT
Asp700I 2642 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 506 GGTACC G^GTACC
AviII 3075 TGCGCA TGC^GCA
BamHI 936 GGATCC G^GATCC
BbsI 4704 GTCTTC GAAGAC(2/6)
BciVI 405 GGATAC GTATCC(6/5)
BglII 884 AGATCT A^GATCT
BlpI 3373 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3373 GCTGAGC GC^T[ACGT]AGC
BpuAI 4704 GTCTTC GAAGAC(2/6)
BsaBI 2162 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 196 GGTCTC GGTCTC(1/5)
BseAI 2026 TCCGGA T^CCGGA
BsmBI 1315 CGTCTC CGTCTC(1/5)
Bsp1407I 1250 TGTACA T^GTACA
BspDI 1000 ATCGAT AT^CGAT
BspEI 2026 TCCGGA T^CCGGA
BspHI 4096 TCATGA T^CATGA
BsrDI 648 CATTGC GCAATG(2/0)
BsrGI 1250 TGTACA T^GTACA
BstAPI 3592 GCACATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 3373 GCTGAGC GC^T[ACGT]AGC
ClaI 1000 ATCGAT AT^CGAT
Eco47III 3096 AGCGCT AGC^GCT
EcoNI 3197 CCTGTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1058 GAATTC G^AATTC
FseI 3901 GGCCGGCC GGCCGG^CC
FspI 3075 TGCGCA TGC^GCA
KpnI 506 GGTACC GGTAC^C
KspI 1845 CCGCGG CCGC^GG
MamI 2162 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2026 TCCGGA T^CCGGA
NruI 2306 TCGCGA TCG^CGA
PaeR7I 1070 CTCGAG C^TCGAG
PshAI 1685 GACGCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 1002 CGATCG CGAT^CG
RcaI 4096 TCATGA T^CATGA
SacII 1845 CCGCGG CCGC^GG
SbfI 4296 CCTGCAGG CCTGCA^GG
SexAI 1343 ACCAGGT A^CC[AT]GGT
SfiI 3901 GGCCGGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 4296 CCTGCAGG CCTGCA^GG
SspBI 1250 TGTACA T^GTACA
XhoI 1070 CTCGAG C^TCGAG
XmnI 2642 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflIII 1119 ACATGT A^C[GA][CT]GT
4866 ACATGT
AspI 4617 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5145 GACCCTGTC
AvrII 640 CCTAGG C^CTAGG
4713 CCTAGG
BbrPI 1664 CACGTG CAC^GTG
3037 CACGTG
BclI 806 TGATCA T^GATCA
2167 TGATCA
BlnI 640 CCTAGG C^CTAGG
4713 CCTAGG
BspLU11I 1119 ACATGT A^CATGT
4866 ACATGT
BssHII 1222 GCGCGC G^CGCGC
2558 GCGCGC
BssSI 880 CACGAG CACGAG(-5/-1)
3287 CACGAG
EagI 1881 CGGCCG C^GGCCG
2018 CGGCCG
EclXI 1881 CGGCCG C^GGCCG
2018 CGGCCG
Eco52I 1881 CGGCCG C^GGCCG
2018 CGGCCG
FbaI 806 TGATCA T^GATCA
2167 TGATCA
PflFI 4617 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5145 GACCCTGTC
PmaCI 1664 CACGTG CAC^GTG
3037 CACGTG
PmlI 1664 CACGTG CAC^GTG
3037 CACGTG
SgrAI 1448 CACCGGCG C[GA]^CCGG[CT]G
2626 CGCCGGCG
Tth111I 4617 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5145 GACCCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbeI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BsaAI BsaHI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI