##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
Acc65I AccII AccIII AclI AgeI
Alw44I Aor51HI ApaI ApaLI AscI
Asp718I AviII BlpI Bpu1102I BsaBI
BsaWI BseAI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BsrBI BsrFI
BsrGI BssHII BstBI BstEII BstPI
BstUI CelII Cfr10I ClaI CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRV FseI FspI
HgaI HpaI KpnI KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NgoMIV NheI NotI NruI
NspV PaeR7I PflMI PinAI PmeI
PshAI Psp1406I PvuI RsrII SacII
SalI SapI SbfI SfiI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI StuI SwaI Van91I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 2379 GACGTC GACGT^C
AhdI 5696 GACATTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 3071 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 4176 CCTAAGG CC^T[ACGT]AGG
AspEI 5696 GACATTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 3030 CTCGGG C^[CT]CG[GA]G
BamHI 1204 GGATCC G^GATCC
BbeI 250 GGCGCC GGCGC^C
BcgI 3044 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2546 TGATCA T^GATCA
BcnI 2859 CCGGG CC^[GC]GG
BglI 253 GCCATCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmBI 2381 CGTCTC CGTCTC(1/5)
BsoBI 3030 CTCGGG C^[CT]CG[GA]G
BssSI 4298 CACGAG CACGAG(-5/-1)
Bsu36I 4176 CCTAAGG CC^T[ACGT]AGG
CfoI 251 GCGC GCG^C
DraII 5130 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 4272 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 5696 GACATTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 4176 CCTAAGG CC^T[ACGT]AGG
EcoO109I 5130 AGGACCC [GA]G^G[ACGT]CC[CT]
FbaI 2546 TGATCA T^GATCA
HaeII 250 GGCGCC [GA]GCGC^[CT]
HhaI 251 GCGC GCG^C
HinP1I 251 GCGC G^CGC
HincII 2763 GTTGAC GT[CT]^[GA]AC
HindII 2763 GTTGAC GT[CT]^[GA]AC
KasI 250 GGCGCC G^GCGCC
MspA1I 5491 CAGCTG C[AC]G^C[GT]G
NarI 250 GGCGCC GG^CGCC
NciI 2859 CCGGG CC^[GC]GG
PpuMI 5130 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 5491 CAGCTG CAG^CTG
SexAI 4971 ACCAGGT A^CC[AT]GGT
SfoI 250 GGCGCC GGC^GCC
XbaI 2593 TCTAGA T^CTAGA
XcmI 441 CCACCTAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 250 GGCGCC G[GA]^CG[CT]C
2379 GACGTC
AflII 1845 CTTAAG C^TTAAG
5469 CTTAAG
AseI 5484 ATTAAT AT^TAAT
5649 ATTAAT
AsnI 5484 ATTAAT AT^TAAT
5649 ATTAAT
AvrII 3112 CCTAGG C^CTAGG
4692 CCTAGG
BanI 250 GGCGCC G^G[CT][GA]CC
928 GGCACC
BbrPI 3822 CACGTG CAC^GTG
4393 CACGTG
BciVI 1262 GGATAC GTATCC(6/5)
4498 GTATCC
BfrI 1845 CTTAAG C^TTAAG
5469 CTTAAG
BlnI 3112 CCTAGG C^CTAGG
4692 CCTAGG
BsaAI 3822 CACGTG [CT]AC^GT[GA]
4393 CACGTG
BsaHI 250 GGCGCC G[GA]^CG[CT]C
2379 GACGTC
Bst1107I 542 GTATAC GTA^TAC
2287 GTATAC
BstAPI 3070 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3373 GCAGGGGTTGC
BstZ17I 542 GTATAC GTA^TAC
2287 GTATAC
EcoT22I 1334 ATGCAT ATGCA^T
1361 ATGCAT
FauI 328 GCGGG CCCGC(4/6)
3214 GCGGG
HapII 2859 CCGG C^CGG
3608 CCGG
Hin1I 250 GGCGCC G[GA]^CG[CT]C
2379 GACGTC
HpaII 2859 CCGG C^CGG
3608 CCGG
MspI 2859 CCGG C^CGG
3608 CCGG
NcoI 1309 CCATGG C^CATGG
3881 CCATGG
NdeI 698 CATATG CA^TATG
4461 CATATG
NsiI 1334 ATGCAT ATGCA^T
1361 ATGCAT
PacI 4648 TTAATTAA TTAAT^TAA
4666 TTAATTAA
PmaCI 3822 CACGTG CAC^GTG
4393 CACGTG
PmlI 3822 CACGTG CAC^GTG
4393 CACGTG
PshBI 5484 ATTAAT AT^TAAT
5649 ATTAAT
PstI 3077 CTGCAG CTGCA^G
3799 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI Asp700I AspHI
AspI AvaII BalI BanII BbsI
BbvCI BbvI BfaI BglII BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspHI BspLU11I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraI DsaI EaeI EarI
EcoRI EcoRII EcoT14I Fnu4HI FokI
HaeIII HindIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV NspI PflFI PleI
RcaI RsaI SacI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SpeI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XhoII XmnI