################################## # NO CUT ENZYMES ( 61 enzymes) # ################################## Acc65I AclI AgeI AocI Aor51HI ApaI AscI Asp718I AspI AvrII BbeI BbvCI BglI BlnI BsiWI BspDI BsrBI BsrFI BssHII BstAPI Bsu36I Cfr10I ClaI CpoI DraIII DrdI Eco47III Eco81I FseI HaeII HpaI KasI KpnI KspI MfeI MluI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PflFI PflMI PinAI Psp1406I PvuI RsrII SacII SbfI SexAI SfiI SfoI SgrAI SnaBI Sse8387I Tth111I Van91I ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AatII 3140 GACGTC GACGT^C AccII 748 CGCG CG^CG AflIII 4418 ACATGT A^C[GA][CT]GT AhdI 5749 GACAATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 370 GTGCAC G^TGCAC ApaLI 370 GTGCAC G^TGCAC AseI 4445 ATTAAT AT^TAAT AsnI 4445 ATTAAT AT^TAAT AspEI 5749 GACAATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 1383 TGCGCA TGC^GCA BbrPI 1759 CACGTG CAC^GTG BcgI 2295 CGAAACTCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 1630 AGATCT A^GATCT BlpI 1919 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1919 GCTGAGC GC^T[ACGT]AGC BsaBI 1167 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 3768 GAGACC GGTCTC(1/5) BsiEI 1812 CGGCCG CG[GA][CT]^CG BspHI 4850 TCATGA T^CATGA BspLU11I 4418 ACATGT A^CATGT BssSI 4238 CTCGTG CACGAG(-5/-1) BstBI 465 TTCGAA TT^CGAA BstEII 1822 GGTGACC G^GT[ACGT]ACC BstPI 1822 GGTGACC G^GT[ACGT]ACC BstUI 748 CGCG CG^CG CelII 1919 GCTGAGC GC^T[ACGT]AGC EagI 1812 CGGCCG C^GGCCG Eam1105I 5749 GACAATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 1812 CGGCCG C^GGCCG Eco52I 1812 CGGCCG C^GGCCG EcoO65I 1822 GGTGACC G^GT[ACGT]ACC FauI 1347 GCGGG CCCGC(4/6) FspI 1383 TGCGCA TGC^GCA MamI 1167 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspA1I 255 CAGCTG C[AC]G^C[GT]G MvnI 748 CGCG CG^CG NcoI 1576 CCATGG C^CATGG NspV 465 TTCGAA TT^CGAA PaeR7I 4095 CTCGAG C^TCGAG PmaCI 1759 CACGTG CAC^GTG PmeI 4195 GTTTAAAC GTTT^AAAC PmlI 1759 CACGTG CAC^GTG PshBI 4445 ATTAAT AT^TAAT PvuII 255 CAGCTG CAG^CTG RcaI 4850 TCATGA T^CATGA SalI 2995 GTCGAC G^TCGAC SapI 1155 GCTCTTC GCTCTTC(1/4) SfuI 465 TTCGAA TT^CGAA SmaI 993 CCCGGG CCC^GGG SpeI 5873 ACTAGT A^CTAGT SphI 728 GCATGC GCATG^C StuI 4663 AGGCCT AGG^CCT XbaI 5684 TCTAGA T^CTAGA XhoI 4095 CTCGAG C^TCGAG XmaCI 993 CCCGGG C^CCGGG XmaI 993 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccIII 1893 TCCGGA T^CCGGA 2509 TCCGGA AvaI 993 CCCGGG C^[CT]CG[GA]G 4095 CTCGAG BamHI 1166 GGATCC G^GATCC 2819 GGATCC BsaAI 1759 CACGTG [CT]AC^GT[GA] 1905 TACGTG BseAI 1893 TCCGGA T^CCGGA 2509 TCCGGA BsmBI 2061 GAGACG CGTCTC(1/5) 2308 GAGACG BsoBI 993 CCCGGG C^[CT]CG[GA]G 4095 CTCGAG BspEI 1893 TCCGGA T^CCGGA 2509 TCCGGA BspMI 593 ACCTGC ACCTGC(4/8) 2503 GCAGGT BstXI 1135 CCAGTTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5487 CCAGCTTTCTGG CfoI 747 GCGC GCG^C 1384 GCGC EcoRV 2809 GATATC GAT^ATC 3350 GATATC EcoT22I 2051 ATGCAT ATGCA^T 3282 ATGCAT HhaI 747 GCGC GCG^C 1384 GCGC HinP1I 747 GCGC G^CGC 1384 GCGC MroI 1893 TCCGGA T^CCGGA 2509 TCCGGA NsiI 2051 ATGCAT ATGCA^T 3282 ATGCAT PpuMI 1455 AGGACCC [GA]G^G[AT]CC[CT] 2440 GGGTCCC PshAI 2177 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 5209 GACCATTGTC PstI 1442 CTGCAG CTGCA^G 1925 CTGCAG ScaI 2519 AGTACT AGT^ACT 2871 AGTACT SwaI 4325 ATTTAAAT ATTT^AAAT 5062 ATTTAAAT ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### AccI AciI AcsI AcyI AfaI AflII AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BalI BanI BanII BbsI BbvI BciVI BclI BcnI BfaI BfrI BmyI BpmI BpuAI BsaHI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BsrDI BsrGI BsrI BssKI Bst1107I BstF5I BstNI BstYI BstZ17I Cac8I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRI EcoRII EcoT14I FbaI Fnu4HI FokI HaeIII HapII HgaI Hin1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI