##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
Acc65I AclI AgeI AocI Aor51HI
ApaI AscI Asp718I AspI AvrII
BbeI BbvCI BglI BlnI BsiWI
BspDI BsrBI BsrFI BssHII BstAPI
Bsu36I Cfr10I ClaI CpoI DraIII
DrdI Eco47III Eco81I FseI HaeII
HpaI KasI KpnI KspI MfeI
MluI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI
PflFI PflMI PinAI Psp1406I PvuI
RsrII SacII SbfI SexAI SfiI
SfoI SgrAI SnaBI Sse8387I Tth111I
Van91I
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 3140 GACGTC GACGT^C
AccII 748 CGCG CG^CG
AflIII 4418 ACATGT A^C[GA][CT]GT
AhdI 5749 GACAATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 370 GTGCAC G^TGCAC
ApaLI 370 GTGCAC G^TGCAC
AseI 4445 ATTAAT AT^TAAT
AsnI 4445 ATTAAT AT^TAAT
AspEI 5749 GACAATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1383 TGCGCA TGC^GCA
BbrPI 1759 CACGTG CAC^GTG
BcgI 2295 CGAAACTCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1630 AGATCT A^GATCT
BlpI 1919 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1919 GCTGAGC GC^T[ACGT]AGC
BsaBI 1167 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3768 GAGACC GGTCTC(1/5)
BsiEI 1812 CGGCCG CG[GA][CT]^CG
BspHI 4850 TCATGA T^CATGA
BspLU11I 4418 ACATGT A^CATGT
BssSI 4238 CTCGTG CACGAG(-5/-1)
BstBI 465 TTCGAA TT^CGAA
BstEII 1822 GGTGACC G^GT[ACGT]ACC
BstPI 1822 GGTGACC G^GT[ACGT]ACC
BstUI 748 CGCG CG^CG
CelII 1919 GCTGAGC GC^T[ACGT]AGC
EagI 1812 CGGCCG C^GGCCG
Eam1105I 5749 GACAATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1812 CGGCCG C^GGCCG
Eco52I 1812 CGGCCG C^GGCCG
EcoO65I 1822 GGTGACC G^GT[ACGT]ACC
FauI 1347 GCGGG CCCGC(4/6)
FspI 1383 TGCGCA TGC^GCA
MamI 1167 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 255 CAGCTG C[AC]G^C[GT]G
MvnI 748 CGCG CG^CG
NcoI 1576 CCATGG C^CATGG
NspV 465 TTCGAA TT^CGAA
PaeR7I 4095 CTCGAG C^TCGAG
PmaCI 1759 CACGTG CAC^GTG
PmeI 4195 GTTTAAAC GTTT^AAAC
PmlI 1759 CACGTG CAC^GTG
PshBI 4445 ATTAAT AT^TAAT
PvuII 255 CAGCTG CAG^CTG
RcaI 4850 TCATGA T^CATGA
SalI 2995 GTCGAC G^TCGAC
SapI 1155 GCTCTTC GCTCTTC(1/4)
SfuI 465 TTCGAA TT^CGAA
SmaI 993 CCCGGG CCC^GGG
SpeI 5873 ACTAGT A^CTAGT
SphI 728 GCATGC GCATG^C
StuI 4663 AGGCCT AGG^CCT
XbaI 5684 TCTAGA T^CTAGA
XhoI 4095 CTCGAG C^TCGAG
XmaCI 993 CCCGGG C^CCGGG
XmaI 993 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccIII 1893 TCCGGA T^CCGGA
2509 TCCGGA
AvaI 993 CCCGGG C^[CT]CG[GA]G
4095 CTCGAG
BamHI 1166 GGATCC G^GATCC
2819 GGATCC
BsaAI 1759 CACGTG [CT]AC^GT[GA]
1905 TACGTG
BseAI 1893 TCCGGA T^CCGGA
2509 TCCGGA
BsmBI 2061 GAGACG CGTCTC(1/5)
2308 GAGACG
BsoBI 993 CCCGGG C^[CT]CG[GA]G
4095 CTCGAG
BspEI 1893 TCCGGA T^CCGGA
2509 TCCGGA
BspMI 593 ACCTGC ACCTGC(4/8)
2503 GCAGGT
BstXI 1135 CCAGTTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5487 CCAGCTTTCTGG
CfoI 747 GCGC GCG^C
1384 GCGC
EcoRV 2809 GATATC GAT^ATC
3350 GATATC
EcoT22I 2051 ATGCAT ATGCA^T
3282 ATGCAT
HhaI 747 GCGC GCG^C
1384 GCGC
HinP1I 747 GCGC G^CGC
1384 GCGC
MroI 1893 TCCGGA T^CCGGA
2509 TCCGGA
NsiI 2051 ATGCAT ATGCA^T
3282 ATGCAT
PpuMI 1455 AGGACCC [GA]G^G[AT]CC[CT]
2440 GGGTCCC
PshAI 2177 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5209 GACCATTGTC
PstI 1442 CTGCAG CTGCA^G
1925 CTGCAG
ScaI 2519 AGTACT AGT^ACT
2871 AGTACT
SwaI 4325 ATTTAAAT ATTT^AAAT
5062 ATTTAAAT
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaII BalI BanI
BanII BbsI BbvI BciVI BclI
BcnI BfaI BfrI BmyI BpmI
BpuAI BsaHI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BsrDI
BsrGI BsrI BssKI Bst1107I BstF5I
BstNI BstYI BstZ17I Cac8I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRI
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeIII HapII HgaI Hin1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmnI