##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AatII Acc65I AccIII AclI Asp718I
AspI BglII BlpI Bpu1102I BseAI
BsiWI BspDI BspEI Bst1107I BstBI
BstZ17I CelII ClaI CpoI DrdI
EcoRV EcoT22I FseI HpaI KpnI
MfeI MluI MroI MunI NheI
NotI NruI NsiI NspV PacI
PflFI Psp1406I PvuI RsrII SalI
ScaI SexAI SfuI SnaBI SphI
StuI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AflII 5285 CTTAAG C^TTAAG
AgeI 30 ACCGGT A^CCGGT
AhdI 4647 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1877 GTGCAC G^TGCAC
ApaLI 1877 GTGCAC G^TGCAC
AscI 2193 GGCGCGCC GG^CGCGCC
AseI 5274 ATTAAT AT^TAAT
AsnI 5274 ATTAAT AT^TAAT
AspEI 4647 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 5444 CCTAGG C^CTAGG
BfrI 5285 CTTAAG C^TTAAG
BlnI 5444 CCTAGG C^CTAGG
BsaBI 2662 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 30 ACCGGT [AT]^CCGG[AT]
BsmBI 1488 GAGACG CGTCTC(1/5)
Bsp1407I 257 TGTACA T^GTACA
BspLU11I 2956 ACATGT A^CATGT
BsrBI 1453 GAGCGG CCGCTC(-3/-3)
BsrGI 257 TGTACA T^GTACA
BstAPI 3150 GCAAGATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1564 GGTGACC G^GT[ACGT]ACC
BstPI 1564 GGTGACC G^GT[ACGT]ACC
DraIII 2600 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 4647 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1564 GGTGACC G^GT[ACGT]ACC
HincII 2118 GTCAAC GT[CT]^[GA]AC
HindII 2118 GTCAAC GT[CT]^[GA]AC
HindIII 4282 AAGCTT A^AGCTT
MamI 2662 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 1314 CATATG CA^TATG
PaeR7I 3859 CTCGAG C^TCGAG
PinAI 30 ACCGGT A^CCGGT
PmeI 5482 GTTTAAAC GTTT^AAAC
PshBI 5274 ATTAAT AT^TAAT
SapI 4907 GAAGAGC GCTCTTC(1/4)
SbfI 1056 CCTGCAGG CCTGCA^GG
SfiI 1264 GGCCCAGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 29 CACCGGTG C[GA]^CCGG[CT]G
Sse8387I 1056 CCTGCAGG CCTGCA^GG
SspBI 257 TGTACA T^GTACA
XhoI 3859 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 2629 GTCTAC GT^[AC][GT]AC
4655 GTCTAC
AflIII 791 ACACGT A^C[GA][CT]GT
2956 ACATGT
BbrPI 553 CACGTG CAC^GTG
792 CACGTG
BcgI 52 CGAGTCTCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1740 CGAAGTCCCTGC
BclI 2335 TGATCA T^GATCA
5383 TGATCA
BsaAI 553 CACGTG [CT]AC^GT[GA]
792 CACGTG
DraI 3143 TTTAAA TTT^AAA
5483 TTTAAA
EagI 1437 CGGCCG C^GGCCG
1828 CGGCCG
EclXI 1437 CGGCCG C^GGCCG
1828 CGGCCG
Eco52I 1437 CGGCCG C^GGCCG
1828 CGGCCG
EcoNI 1539 CCTGGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5537 CCTCTTTCAGG
EcoRI 1417 GAATTC G^AATTC
2320 GAATTC
FbaI 2335 TGATCA T^GATCA
5383 TGATCA
PmaCI 553 CACGTG CAC^GTG
792 CACGTG
PmlI 553 CACGTG CAC^GTG
792 CACGTG
PshAI 1705 GACAGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3981 GACAGGTGTC
SpeI 3553 ACTAGT A^CTAGT
4697 ACTAGT
XcmI 3683 CCAGAAGGCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5024 CCAGAGGGCACATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 150 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI Aor51HI
ApaI ApoI Asp700I AspHI AvaI
AvaII AviII BalI BamHI BanI
BanII BbeI BbsI BbvCI BbvI
BciVI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspHI BspMI BsrDI BsrFI
BsrI BssHII BssKI BssSI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco47III Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RcaI RsaI
SacI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmaCI XmaI XmnI