##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Aor51HI AscI AviII AvrII BbrPI
BcgI BciVI BclI BfrI BlnI
BsaAI BsaI BseAI BsgI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BsrBI BsrGI BssHII BstAPI BstEII
BstPI BstXI ClaI CpoI EagI
EclXI Eco47III Eco52I EcoO65I FbaI
FseI FspI HgaI HpaI KspI
MluI MroI NheI NotI NruI
PacI PaeR7I PinAI PmaCI PmlI
Psp1406I PvuI RsrII SacII SalI
SexAI SfiI SgrAI SmaI SnaBI
SspBI StuI SwaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
Acc65I 3226 GGTACC G^GTACC
AccII 570 CGCG CG^CG
AcyI 3331 GGCGCC G[GA]^CG[CT]C
AhdI 2836 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 792 CCTGAGG CC^T[ACGT]AGG
ApaI 449 GGGCCC GGGCC^C
AseI 65 ATTAAT AT^TAAT
AsnI 65 ATTAAT AT^TAAT
Asp718I 3226 GGTACC G^GTACC
AspEI 2836 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1285 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 637 TGGCCA TGG^CCA
BbeI 3331 GGCGCC GGCGC^C
BsaHI 3331 GGCGCC G[GA]^CG[CT]C
BsaWI 490 TCCGGT [AT]^CCGG[AT]
BsmI 3844 GCATTC GAATGC(1/-1)
BsrFI 893 GCCGGC [GA]^CCGG[CT]
BssSI 910 CTCGTG CACGAG(-5/-1)
Bst1107I 5305 GTATAC GTA^TAC
BstBI 4693 TTCGAA TT^CGAA
BstUI 570 CGCG CG^CG
BstZ17I 5305 GTATAC GTA^TAC
Bsu36I 792 CCTGAGG CC^T[ACGT]AGG
Cfr10I 893 GCCGGC [GA]^CCGG[CT]
DraIII 5262 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 957 GACCTTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2836 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 792 CCTGAGG CC^T[ACGT]AGG
EcoNI 4861 CCTTTTCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 3331 GGCGCC [GA]GCGC^[CT]
Hin1I 3331 GGCGCC G[GA]^CG[CT]C
HincII 4931 GTCAAC GT[CT]^[GA]AC
HindII 4931 GTCAAC GT[CT]^[GA]AC
KasI 3331 GGCGCC G^GCGCC
KpnI 3226 GGTACC GGTAC^C
MfeI 4936 CAATTG C^AATTG
MluNI 637 TGGCCA TGG^CCA
MscI 637 TGGCCA TGG^CCA
MunI 4936 CAATTG C^AATTG
MvnI 570 CGCG CG^CG
NaeI 893 GCCGGC GCC^GGC
NarI 3331 GGCGCC GG^CGCC
NdeI 2242 CATATG CA^TATG
NgoMIV 893 GCCGGC G^CCGGC
NspV 4693 TTCGAA TT^CGAA
PflFI 1285 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmeI 1739 GTTTAAAC GTTT^AAAC
PpuMI 1147 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 3889 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 65 ATTAAT AT^TAAT
SacI 5173 GAGCTC GAGCT^C
SbfI 827 CCTGCAGG CCTGCA^GG
SfoI 3331 GGCGCC GGC^GCC
SfuI 4693 TTCGAA TT^CGAA
SphI 4746 GCATGC GCATG^C
Sse8387I 827 CCTGCAGG CCTGCA^GG
Tth111I 1285 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3486 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AccI 5305 GTATAC GT^[AC][GT]AC
5665 GTAGAC
Alw44I 2214 GTGCAC G^TGCAC
2686 GTGCAC
ApaLI 2214 GTGCAC G^TGCAC
2686 GTGCAC
BbvCI 1952 CCTCAGC CCTCAGC(-5/-2)
4481 CCTCAGC
BglI 464 GCCCCTTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1163 GCCACCTCGGC
BsaBI 436 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1096 GATCCAGATC
BseRI 1196 CTCCTC GAGGAG(10/8)
3151 GAGGAG
DsaI 350 CCATGG C^C[GA][CT]GG
3921 CCATGG
EcoRV 3044 GATATC GAT^ATC
4112 GATATC
MamI 436 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1096 GATCCAGATC
NcoI 350 CCATGG C^CATGG
3921 CCATGG
ScaI 574 AGTACT AGT^ACT
1011 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BamHI BanI BanII
BbsI BbvI BcnI BfaI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BspLU11I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
EaeI EarI EcoO109I EcoRI EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PstI PvuII RcaI RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SpeI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmnI