##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AccIII AclI AgeI Aor51HI Asp700I
BamHI BbeI BciVI BsaBI BsaWI
BseAI BsiEI BspDI BspEI BsrDI
BstBI ClaI CpoI EagI EclXI
Eco47III Eco52I EcoRV EcoT22I FseI
HindIII HpaI KasI KspI MamI
MfeI MluI MroI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PaeR7I PinAI PmeI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I SwaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 1028 GACGTC GACGT^C
Acc65I 3281 GGTACC G^GTACC
AccI 2588 GTATAC GT^[AC][GT]AC
AcyI 1028 GACGTC G[GA]^CG[CT]C
AflII 2169 CTTAAG C^TTAAG
AhdI 219 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AscI 113 GGCGCGCC GG^CGCGCC
AseI 1558 ATTAAT AT^TAAT
AsnI 1558 ATTAAT AT^TAAT
Asp718I 3281 GGTACC G^GTACC
AspEI 219 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1578 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4000 TGCGCA TGC^GCA
BcgI 1890 GCAGCCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3129 TGATCA T^GATCA
BfrI 2169 CTTAAG C^TTAAG
BglII 631 AGATCT A^GATCT
BsaHI 1028 GACGTC G[GA]^CG[CT]C
BsiWI 3877 CGTACG C^GTACG
BsmI 747 GCATTC GAATGC(1/-1)
Bsp1407I 4114 TGTACA T^GTACA
BspLU11I 2394 ACATGT A^CATGT
BsrGI 4114 TGTACA T^GTACA
BssHII 114 GCGCGC G^CGCGC
Bst1107I 2588 GTATAC GTA^TAC
BstYI 631 AGATCT [GA]^GATC[CT]
BstZ17I 2588 GTATAC GTA^TAC
Eam1105I 219 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 4263 CTCTTC CTCTTC(1/4)
EcoRI 2005 GAATTC G^AATTC
FbaI 3129 TGATCA T^GATCA
FspI 4000 TGCGCA TGC^GCA
HgaI 3659 GACGC GACGC(5/10)
Hin1I 1028 GACGTC G[GA]^CG[CT]C
HincII 2298 GTTGAC GT[CT]^[GA]AC
HindII 2298 GTTGAC GT[CT]^[GA]AC
KpnI 3281 GGTACC GGTAC^C
Ksp632I 4263 CTCTTC CTCTTC(1/4)
MflI 631 AGATCT [GA]^GATC[CT]
PacI 1555 TTAATTAA TTAAT^TAA
PflFI 1578 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1310 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1558 ATTAAT AT^TAAT
SfiI 401 GGCCCACCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 2123 ACTAGT A^CTAGT
SphI 1681 GCATGC GCATG^C
SspBI 4114 TGTACA T^GTACA
SspI 1716 AATATT AAT^ATT
Tth111I 1578 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1214 TCTAGA T^CTAGA
XhoII 631 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Alw44I 2826 GTGCAC G^TGCAC
3307 GTGCAC
AlwI 1799 GATCC GGATC(4/5)
1269 GGATC
ApaLI 2826 GTGCAC G^TGCAC
3307 GTGCAC
AvrII 1487 CCTAGG C^CTAGG
3287 CCTAGG
BbrPI 2018 CACGTG CAC^GTG
3032 CACGTG
BbsI 2111 GAAGAC GAAGAC(2/6)
3071 GTCTTC
BlnI 1487 CCTAGG C^CTAGG
3287 CCTAGG
BlpI 1978 GCTCAGC GC^T[ACGT]AGC
2307 GCTCAGC
Bpu1102I 1978 GCTCAGC GC^T[ACGT]AGC
2307 GCTCAGC
BpuAI 2111 GAAGAC GAAGAC(2/6)
3071 GTCTTC
BsmBI 1030 CGTCTC CGTCTC(1/5)
2474 GAGACG
BspHI 178 TCATGA T^CATGA
4192 TCATGA
BstAPI 3031 GCACGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3309 GCACTGGCTGC
CelII 1978 GCTCAGC GC^T[ACGT]AGC
2307 GCTCAGC
DrdI 1909 GACCAAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4549 GACCCCCATGTC
PmaCI 2018 CACGTG CAC^GTG
3032 CACGTG
PmlI 2018 CACGTG CAC^GTG
3032 CACGTG
RcaI 178 TCATGA T^CATGA
4192 TCATGA
SacI 3269 GAGCTC GAGCT^C
4233 GAGCTC
SexAI 1579 ACCAGGT A^CC[AT]GGT
4039 ACCAGGT
TfiI 2678 GATTC G^A[AT]TC
4370 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwNI AocI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BsaAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BssSI BstEII BstF5I BstNI BstPI
BstUI BstXI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI