################################## # NO CUT ENZYMES ( 63 enzymes) # ################################## AccIII AclI AgeI Aor51HI Asp700I BamHI BbeI BciVI BsaBI BsaWI BseAI BsiEI BspDI BspEI BsrDI BstBI ClaI CpoI EagI EclXI Eco47III Eco52I EcoRV EcoT22I FseI HindIII HpaI KasI KspI MamI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI NsiI NspV PaeR7I PinAI PmeI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SwaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AatII 1028 GACGTC GACGT^C Acc65I 3281 GGTACC G^GTACC AccI 2588 GTATAC GT^[AC][GT]AC AcyI 1028 GACGTC G[GA]^CG[CT]C AflII 2169 CTTAAG C^TTAAG AhdI 219 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AscI 113 GGCGCGCC GG^CGCGCC AseI 1558 ATTAAT AT^TAAT AsnI 1558 ATTAAT AT^TAAT Asp718I 3281 GGTACC G^GTACC AspEI 219 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1578 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 4000 TGCGCA TGC^GCA BcgI 1890 GCAGCCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 3129 TGATCA T^GATCA BfrI 2169 CTTAAG C^TTAAG BglII 631 AGATCT A^GATCT BsaHI 1028 GACGTC G[GA]^CG[CT]C BsiWI 3877 CGTACG C^GTACG BsmI 747 GCATTC GAATGC(1/-1) Bsp1407I 4114 TGTACA T^GTACA BspLU11I 2394 ACATGT A^CATGT BsrGI 4114 TGTACA T^GTACA BssHII 114 GCGCGC G^CGCGC Bst1107I 2588 GTATAC GTA^TAC BstYI 631 AGATCT [GA]^GATC[CT] BstZ17I 2588 GTATAC GTA^TAC Eam1105I 219 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 4263 CTCTTC CTCTTC(1/4) EcoRI 2005 GAATTC G^AATTC FbaI 3129 TGATCA T^GATCA FspI 4000 TGCGCA TGC^GCA HgaI 3659 GACGC GACGC(5/10) Hin1I 1028 GACGTC G[GA]^CG[CT]C HincII 2298 GTTGAC GT[CT]^[GA]AC HindII 2298 GTTGAC GT[CT]^[GA]AC KpnI 3281 GGTACC GGTAC^C Ksp632I 4263 CTCTTC CTCTTC(1/4) MflI 631 AGATCT [GA]^GATC[CT] PacI 1555 TTAATTAA TTAAT^TAA PflFI 1578 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 1310 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1558 ATTAAT AT^TAAT SfiI 401 GGCCCACCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SpeI 2123 ACTAGT A^CTAGT SphI 1681 GCATGC GCATG^C SspBI 4114 TGTACA T^GTACA SspI 1716 AATATT AAT^ATT Tth111I 1578 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 1214 TCTAGA T^CTAGA XhoII 631 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### Alw44I 2826 GTGCAC G^TGCAC 3307 GTGCAC AlwI 1799 GATCC GGATC(4/5) 1269 GGATC ApaLI 2826 GTGCAC G^TGCAC 3307 GTGCAC AvrII 1487 CCTAGG C^CTAGG 3287 CCTAGG BbrPI 2018 CACGTG CAC^GTG 3032 CACGTG BbsI 2111 GAAGAC GAAGAC(2/6) 3071 GTCTTC BlnI 1487 CCTAGG C^CTAGG 3287 CCTAGG BlpI 1978 GCTCAGC GC^T[ACGT]AGC 2307 GCTCAGC Bpu1102I 1978 GCTCAGC GC^T[ACGT]AGC 2307 GCTCAGC BpuAI 2111 GAAGAC GAAGAC(2/6) 3071 GTCTTC BsmBI 1030 CGTCTC CGTCTC(1/5) 2474 GAGACG BspHI 178 TCATGA T^CATGA 4192 TCATGA BstAPI 3031 GCACGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3309 GCACTGGCTGC CelII 1978 GCTCAGC GC^T[ACGT]AGC 2307 GCTCAGC DrdI 1909 GACCAAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4549 GACCCCCATGTC PmaCI 2018 CACGTG CAC^GTG 3032 CACGTG PmlI 2018 CACGTG CAC^GTG 3032 CACGTG RcaI 178 TCATGA T^CATGA 4192 TCATGA SacI 3269 GAGCTC GAGCT^C 4233 GAGCTC SexAI 1579 ACCAGGT A^CC[AT]GGT 4039 ACCAGGT TfiI 2678 GATTC G^A[AT]TC 4370 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 123 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwNI AocI ApaI ApoI AspHI AvaI AvaII BalI BanI BanII BbvCI BbvI BcnI BfaI BglI BmyI BpmI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BssSI BstEII BstF5I BstNI BstPI BstUI BstXI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI