##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII AclI AflII AgeI AscI
AseI AsnI BamHI BbeI BcgI
BfrI BglII BsiWI BspDI BspLU11I
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI DraI EagI EclXI Eco52I
EcoT22I FseI HindIII HpaI KasI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I RsrII
SalI SbfI ScaI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 4802 TCCGGA T^CCGGA
AflIII 5037 ACGCGT A^C[GA][CT]GT
AspI 3184 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 5159 TGCGCA TGC^GCA
AvrII 558 CCTAGG C^CTAGG
BlnI 558 CCTAGG C^CTAGG
BsaBI 1909 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 4802 TCCGGA T^CCGGA
BsmBI 2601 CGTCTC CGTCTC(1/5)
BsmI 3394 GCATTC GAATGC(1/-1)
BspEI 4802 TCCGGA T^CCGGA
BspHI 3366 TCATGA T^CATGA
BssHII 3886 GCGCGC G^CGCGC
BstEII 1015 GGTCACC G^GT[ACGT]ACC
BstPI 1015 GGTCACC G^GT[ACGT]ACC
DrdI 781 GACCCGGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1015 GGTCACC G^GT[ACGT]ACC
EcoRV 3757 GATATC GAT^ATC
FspI 5159 TGCGCA TGC^GCA
KspI 5138 CCGCGG CCGC^GG
MamI 1909 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4172 CAATTG C^AATTG
MluI 5037 ACGCGT A^CGCGT
MroI 4802 TCCGGA T^CCGGA
MunI 4172 CAATTG C^AATTG
PflFI 3184 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 4752 CGATCG CGAT^CG
RcaI 3366 TCATGA T^CATGA
SacII 5138 CCGCGG CCGC^GG
SfiI 4632 GGCCATGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspI 328 AATATT AAT^ATT
Tth111I 3184 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2262 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 4049 GGTACC G^GTACC
4940 GGTACC
AccI 2448 GTCTAC GT^[AC][GT]AC
4849 GTCTAC
AhdI 3724 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5143 GACCCTCTGTC
Alw44I 3895 GTGCAC G^TGCAC
4159 GTGCAC
ApaLI 3895 GTGCAC G^TGCAC
4159 GTGCAC
Asp700I 2691 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2992 GAATTCCTTC
Asp718I 4049 GGTACC G^GTACC
4940 GGTACC
AspEI 3724 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5143 GACCCTCTGTC
BalI 1640 TGGCCA TGG^CCA
4658 TGGCCA
BbrPI 1987 CACGTG CAC^GTG
3919 CACGTG
BciVI 521 GGATAC GTATCC(6/5)
1908 GGATAC
BclI 1914 TGATCA T^GATCA
2021 TGATCA
BsiEI 3077 CGACCG CG[GA][CT]^CG
4752 CGATCG
BsrDI 902 CATTGC GCAATG(2/0)
1385 CATTGC
BstAPI 2085 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2976 GCAGACGCTGC
Eam1105I 3724 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5143 GACCCTCTGTC
EcoRI 671 GAATTC G^AATTC
2992 GAATTC
FbaI 1914 TGATCA T^GATCA
2021 TGATCA
HincII 4081 GTCAAC GT[CT]^[GA]AC
4525 GTCAAC
HindII 4081 GTCAAC GT[CT]^[GA]AC
4525 GTCAAC
KpnI 4049 GGTACC GGTAC^C
4940 GGTACC
MluNI 1640 TGGCCA TGG^CCA
4658 TGGCCA
MscI 1640 TGGCCA TGG^CCA
4658 TGGCCA
MseI 4247 TTAA T^TAA
4835 TTAA
NaeI 3118 GCCGGC GCC^GGC
3161 GCCGGC
NgoMIV 3118 GCCGGC G^CCGGC
3161 GCCGGC
NspI 1001 GCATGC [GA]CATG^[CT]
4359 GCATGC
PmaCI 1987 CACGTG CAC^GTG
3919 CACGTG
PmlI 1987 CACGTG CAC^GTG
3919 CACGTG
SexAI 3611 ACCAGGT A^CC[AT]GGT
4610 ACCTGGT
SmlI 3901 CTCAAG C^T[CT][GA]AG
3943 CTCAAG
SphI 1001 GCATGC GCATG^C
4359 GCATGC
Tru9I 4247 TTAA T^TAA
4835 TTAA
XmnI 2691 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2992 GAATTCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI Aor51HI
ApaI ApoI AspHI AvaI AvaII
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspMI BsrBI
BsrFI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco47III Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SspBI StuI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI