################################## # NO CUT ENZYMES ( 56 enzymes) # ################################## AatII AclI AflII AgeI AscI AseI AsnI BamHI BbeI BcgI BfrI BglII BsiWI BspDI BspLU11I BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraI EagI EclXI Eco52I EcoT22I FseI HindIII HpaI KasI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I RsrII SalI SbfI ScaI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SwaI XhoI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AccIII 4802 TCCGGA T^CCGGA AflIII 5037 ACGCGT A^C[GA][CT]GT AspI 3184 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 5159 TGCGCA TGC^GCA AvrII 558 CCTAGG C^CTAGG BlnI 558 CCTAGG C^CTAGG BsaBI 1909 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 4802 TCCGGA T^CCGGA BsmBI 2601 CGTCTC CGTCTC(1/5) BsmI 3394 GCATTC GAATGC(1/-1) BspEI 4802 TCCGGA T^CCGGA BspHI 3366 TCATGA T^CATGA BssHII 3886 GCGCGC G^CGCGC BstEII 1015 GGTCACC G^GT[ACGT]ACC BstPI 1015 GGTCACC G^GT[ACGT]ACC DrdI 781 GACCCGGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 1015 GGTCACC G^GT[ACGT]ACC EcoRV 3757 GATATC GAT^ATC FspI 5159 TGCGCA TGC^GCA KspI 5138 CCGCGG CCGC^GG MamI 1909 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 4172 CAATTG C^AATTG MluI 5037 ACGCGT A^CGCGT MroI 4802 TCCGGA T^CCGGA MunI 4172 CAATTG C^AATTG PflFI 3184 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuI 4752 CGATCG CGAT^CG RcaI 3366 TCATGA T^CATGA SacII 5138 CCGCGG CCGC^GG SfiI 4632 GGCCATGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SspI 328 AATATT AAT^ATT Tth111I 3184 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 2262 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### Acc65I 4049 GGTACC G^GTACC 4940 GGTACC AccI 2448 GTCTAC GT^[AC][GT]AC 4849 GTCTAC AhdI 3724 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5143 GACCCTCTGTC Alw44I 3895 GTGCAC G^TGCAC 4159 GTGCAC ApaLI 3895 GTGCAC G^TGCAC 4159 GTGCAC Asp700I 2691 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2992 GAATTCCTTC Asp718I 4049 GGTACC G^GTACC 4940 GGTACC AspEI 3724 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5143 GACCCTCTGTC BalI 1640 TGGCCA TGG^CCA 4658 TGGCCA BbrPI 1987 CACGTG CAC^GTG 3919 CACGTG BciVI 521 GGATAC GTATCC(6/5) 1908 GGATAC BclI 1914 TGATCA T^GATCA 2021 TGATCA BsiEI 3077 CGACCG CG[GA][CT]^CG 4752 CGATCG BsrDI 902 CATTGC GCAATG(2/0) 1385 CATTGC BstAPI 2085 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2976 GCAGACGCTGC Eam1105I 3724 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5143 GACCCTCTGTC EcoRI 671 GAATTC G^AATTC 2992 GAATTC FbaI 1914 TGATCA T^GATCA 2021 TGATCA HincII 4081 GTCAAC GT[CT]^[GA]AC 4525 GTCAAC HindII 4081 GTCAAC GT[CT]^[GA]AC 4525 GTCAAC KpnI 4049 GGTACC GGTAC^C 4940 GGTACC MluNI 1640 TGGCCA TGG^CCA 4658 TGGCCA MscI 1640 TGGCCA TGG^CCA 4658 TGGCCA MseI 4247 TTAA T^TAA 4835 TTAA NaeI 3118 GCCGGC GCC^GGC 3161 GCCGGC NgoMIV 3118 GCCGGC G^CCGGC 3161 GCCGGC NspI 1001 GCATGC [GA]CATG^[CT] 4359 GCATGC PmaCI 1987 CACGTG CAC^GTG 3919 CACGTG PmlI 1987 CACGTG CAC^GTG 3919 CACGTG SexAI 3611 ACCAGGT A^CC[AT]GGT 4610 ACCTGGT SmlI 3901 CTCAAG C^T[CT][GA]AG 3943 CTCAAG SphI 1001 GCATGC GCATG^C 4359 GCATGC Tru9I 4247 TTAA T^TAA 4835 TTAA XmnI 2691 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2992 GAATTCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 135 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI Aor51HI ApaI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrBI BsrFI BsrGI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco47III Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SspBI StuI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI