##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AccI AccIII AclI AgeI
Aor51HI AscI AseI AsnI Asp700I
AviII BamHI BcgI BciVI BsaBI
BsaWI BseAI BsiEI BsiWI BspDI
BspEI BspHI BsrFI BssHII Bst1107I
BstBI BstZ17I Cfr10I ClaI DrdI
EagI EclXI Eco47III Eco52I EcoRI
FseI FspI HincII HindII HindIII
HpaI KspI MamI MfeI MluI
MroI MunI NaeI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshBI Psp1406I
PvuI RcaI SacII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SwaI XbaI
XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 4035 GGTACC G^GTACC
AcyI 1980 GGCGCC G[GA]^CG[CT]C
AflII 910 CTTAAG C^TTAAG
Alw44I 1214 GTGCAC G^TGCAC
ApaLI 1214 GTGCAC G^TGCAC
Asp718I 4035 GGTACC G^GTACC
AvrII 2564 CCTAGG C^CTAGG
BalI 3068 TGGCCA TGG^CCA
BbeI 1980 GGCGCC GGCGC^C
BfrI 910 CTTAAG C^TTAAG
BglII 3645 AGATCT A^GATCT
BlnI 2564 CCTAGG C^CTAGG
BsaHI 1980 GGCGCC G[GA]^CG[CT]C
Bsp1407I 3630 TGTACA T^GTACA
BspLU11I 429 ACATGT A^CATGT
BsrBI 1076 CCGCTC CCGCTC(-3/-3)
BsrGI 3630 TGTACA T^GTACA
BssSI 5452 CTCGTG CACGAG(-5/-1)
BstYI 3645 AGATCT [GA]^GATC[CT]
CpoI 1028 CGGTCCG CG^G[AT]CCG
DraIII 2414 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 3192 GATATC GAT^ATC
EcoT22I 3403 ATGCAT ATGCA^T
HaeII 1980 GGCGCC [GA]GCGC^[CT]
Hin1I 1980 GGCGCC G[GA]^CG[CT]C
KasI 1980 GGCGCC G^GCGCC
KpnI 4035 GGTACC GGTAC^C
MflI 3645 AGATCT [GA]^GATC[CT]
MluNI 3068 TGGCCA TGG^CCA
MscI 3068 TGGCCA TGG^CCA
NarI 1980 GGCGCC GG^CGCC
NcoI 4031 CCATGG C^CATGG
NsiI 3403 ATGCAT ATGCA^T
RsrII 1028 CGGTCCG CG^G[AT]CCG
SapI 5175 GAAGAGC GCTCTTC(1/4)
SfoI 1980 GGCGCC GGC^GCC
SphI 5887 GCATGC GCATG^C
SspBI 3630 TGTACA T^GTACA
XhoII 3645 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflIII 429 ACATGT A^C[GA][CT]GT
5549 ACACGT
AhdI 1060 GACTCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2339 GACCCACCGTC
AlwNI 1001 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
3530 CAGGTGCTG
AspEI 1060 GACTCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2339 GACCCACCGTC
AspI 365 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2099 GACTCCGTC
BsmI 184 GAATGC GAATGC(1/-1)
3401 GAATGC
BsrDI 1381 CATTGC GCAATG(2/0)
3680 GCAATG
BstEII 1391 GGTCACC G^GT[ACGT]ACC
5446 GGTAACC
BstPI 1391 GGTCACC G^GT[ACGT]ACC
5446 GGTAACC
BstXI 4509 CCAGGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5555 CCACGTGATTGG
DsaI 4031 CCATGG C^C[GA][CT]GG
4909 CCGTGG
Eam1105I 1060 GACTCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2339 GACCCACCGTC
EcoNI 3866 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5451 CCTCGTGGAGG
EcoO65I 1391 GGTCACC G^GT[ACGT]ACC
5446 GGTAACC
PflFI 365 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2099 GACTCCGTC
PflMI 1000 CCAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3435 CCATCCTCTGG
SacI 2322 GAGCTC GAGCT^C
5734 GAGCTC
SspI 127 AATATT AAT^ATT
595 AATATT
Tth111I 365 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2099 GACTCCGTC
Van91I 1000 CCAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3435 CCATCCTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AocI ApaI ApoI AspHI
AvaI AvaII BanI BanII BbrPI
BbsI BbvCI BbvI BclI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstUI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PmaCI PmlI PpuMI PshAI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XmaCI
XmaI