################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## AatII AccI AccIII AclI AgeI Aor51HI AscI AseI AsnI Asp700I AviII BamHI BcgI BciVI BsaBI BsaWI BseAI BsiEI BsiWI BspDI BspEI BspHI BsrFI BssHII Bst1107I BstBI BstZ17I Cfr10I ClaI DrdI EagI EclXI Eco47III Eco52I EcoRI FseI FspI HincII HindII HindIII HpaI KspI MamI MfeI MluI MroI MunI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RcaI SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SwaI XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Acc65I 4035 GGTACC G^GTACC AcyI 1980 GGCGCC G[GA]^CG[CT]C AflII 910 CTTAAG C^TTAAG Alw44I 1214 GTGCAC G^TGCAC ApaLI 1214 GTGCAC G^TGCAC Asp718I 4035 GGTACC G^GTACC AvrII 2564 CCTAGG C^CTAGG BalI 3068 TGGCCA TGG^CCA BbeI 1980 GGCGCC GGCGC^C BfrI 910 CTTAAG C^TTAAG BglII 3645 AGATCT A^GATCT BlnI 2564 CCTAGG C^CTAGG BsaHI 1980 GGCGCC G[GA]^CG[CT]C Bsp1407I 3630 TGTACA T^GTACA BspLU11I 429 ACATGT A^CATGT BsrBI 1076 CCGCTC CCGCTC(-3/-3) BsrGI 3630 TGTACA T^GTACA BssSI 5452 CTCGTG CACGAG(-5/-1) BstYI 3645 AGATCT [GA]^GATC[CT] CpoI 1028 CGGTCCG CG^G[AT]CCG DraIII 2414 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRV 3192 GATATC GAT^ATC EcoT22I 3403 ATGCAT ATGCA^T HaeII 1980 GGCGCC [GA]GCGC^[CT] Hin1I 1980 GGCGCC G[GA]^CG[CT]C KasI 1980 GGCGCC G^GCGCC KpnI 4035 GGTACC GGTAC^C MflI 3645 AGATCT [GA]^GATC[CT] MluNI 3068 TGGCCA TGG^CCA MscI 3068 TGGCCA TGG^CCA NarI 1980 GGCGCC GG^CGCC NcoI 4031 CCATGG C^CATGG NsiI 3403 ATGCAT ATGCA^T RsrII 1028 CGGTCCG CG^G[AT]CCG SapI 5175 GAAGAGC GCTCTTC(1/4) SfoI 1980 GGCGCC GGC^GCC SphI 5887 GCATGC GCATG^C SspBI 3630 TGTACA T^GTACA XhoII 3645 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AflIII 429 ACATGT A^C[GA][CT]GT 5549 ACACGT AhdI 1060 GACTCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2339 GACCCACCGTC AlwNI 1001 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG 3530 CAGGTGCTG AspEI 1060 GACTCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2339 GACCCACCGTC AspI 365 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2099 GACTCCGTC BsmI 184 GAATGC GAATGC(1/-1) 3401 GAATGC BsrDI 1381 CATTGC GCAATG(2/0) 3680 GCAATG BstEII 1391 GGTCACC G^GT[ACGT]ACC 5446 GGTAACC BstPI 1391 GGTCACC G^GT[ACGT]ACC 5446 GGTAACC BstXI 4509 CCAGGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5555 CCACGTGATTGG DsaI 4031 CCATGG C^C[GA][CT]GG 4909 CCGTGG Eam1105I 1060 GACTCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2339 GACCCACCGTC EcoNI 3866 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 5451 CCTCGTGGAGG EcoO65I 1391 GGTCACC G^GT[ACGT]ACC 5446 GGTAACC PflFI 365 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2099 GACTCCGTC PflMI 1000 CCAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3435 CCATCCTCTGG SacI 2322 GAGCTC GAGCT^C 5734 GAGCTC SspI 127 AATATT AAT^ATT 595 AATATT Tth111I 365 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2099 GACTCCGTC Van91I 1000 CCAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3435 CCATCCTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AocI ApaI ApoI AspHI AvaI AvaII BanI BanII BbrPI BbsI BbvCI BbvI BclI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstUI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PmaCI PmlI PpuMI PshAI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XmaCI XmaI