##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII Acc65I AgeI AhdI Aor51HI
AscI AseI AsnI Asp718I AspEI
BciVI BsiEI BsiWI BspDI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
KpnI KspI MfeI MluI MunI
NheI NotI NruI NspV PacI
PinAI PmeI PshBI PvuI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SwaI XbaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccIII 923 TCCGGA T^CCGGA
AclI 465 AACGTT AA^CGTT
AflII 2128 CTTAAG C^TTAAG
ApaI 5728 GGGCCC GGGCC^C
AspI 1894 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4312 TGCGCA TGC^GCA
AvrII 2886 CCTAGG C^CTAGG
BbeI 5878 GGCGCC GGCGC^C
BbrPI 5298 CACGTG CAC^GTG
BcgI 1981 GCAGGCGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4845 TGATCA T^GATCA
BfrI 2128 CTTAAG C^TTAAG
BlnI 2886 CCTAGG C^CTAGG
BsaBI 1391 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 923 TCCGGA [AT]^CCGG[AT]
BseAI 923 TCCGGA T^CCGGA
Bsp1407I 1920 TGTACA T^GTACA
BspEI 923 TCCGGA T^CCGGA
BspHI 5123 TCATGA T^CATGA
BsrBI 698 CCGCTC CCGCTC(-3/-3)
BsrDI 476 GCAATG GCAATG(2/0)
BsrGI 1920 TGTACA T^GTACA
BssHII 113 GCGCGC G^CGCGC
BstXI 4058 CCAAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 86 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
FbaI 4845 TGATCA T^GATCA
FseI 2854 GGCCGGCC GGCCGG^CC
FspI 4312 TGCGCA TGC^GCA
HpaI 4603 GTTAAC GTT^AAC
KasI 5878 GGCGCC G^GCGCC
MamI 1391 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 923 TCCGGA T^CCGGA
NarI 5878 GGCGCC GG^CGCC
NcoI 1204 CCATGG C^CATGG
NdeI 5224 CATATG CA^TATG
PaeR7I 1728 CTCGAG C^TCGAG
PflFI 1894 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 5298 CACGTG CAC^GTG
PmlI 5298 CACGTG CAC^GTG
PshAI 766 GACGACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 465 AACGTT AA^CGTT
RcaI 5123 TCATGA T^CATGA
ScaI 904 AGTACT AGT^ACT
SfoI 5878 GGCGCC GGC^GCC
SspBI 1920 TGTACA T^GTACA
SspI 5636 AATATT AAT^ATT
Tth111I 1894 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2894 CCACTGGCCACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1728 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Asp700I 809 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5437 GAATGCTTTC
BglII 3009 AGATCT A^GATCT
4203 AGATCT
BlpI 2953 GCTCAGC GC^T[ACGT]AGC
4379 GCTCAGC
Bpu1102I 2953 GCTCAGC GC^T[ACGT]AGC
4379 GCTCAGC
BsmBI 27 CGTCTC CGTCTC(1/5)
2199 GAGACG
BstAPI 141 GCAGCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2310 GCAAAGATTGC
CelII 2953 GCTCAGC GC^T[ACGT]AGC
4379 GCTCAGC
DraI 5063 TTTAAA TTT^AAA
5548 TTTAAA
EcoNI 1160 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1778 CCTGACCCAGG
EcoT22I 4241 ATGCAT ATGCA^T
5680 ATGCAT
HincII 1311 GTCAAC GT[CT]^[GA]AC
4603 GTTAAC
HindII 1311 GTCAAC GT[CT]^[GA]AC
4603 GTTAAC
NsiI 4241 ATGCAT ATGCA^T
5680 ATGCAT
SacI 2951 GAGCTC GAGCT^C
4022 GAGCTC
SmaI 7 CCCGGG CCC^GGG
5930 CCCGGG
StuI 3647 AGGCCT AGG^CCT
5540 AGGCCT
XmaCI 7 CCCGGG C^CCGGG
5930 CCCGGG
XmaI 7 CCCGGG C^CCGGG
5930 CCCGGG
XmnI 809 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5437 GAATGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI AocI ApaLI ApoI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspLU11I
BspMI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII