##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
AatII AflII AgeI Aor51HI AscI
AseI AsnI Asp700I AvrII BfrI
BlnI BsaBI BspDI ClaI CpoI
DrdI Eco47III EcoRI EcoRV HindIII
HpaI MamI MfeI MluI MunI
NdeI NotI NruI PacI PaeR7I
PinAI PmeI PshBI PvuI RsrII
SalI SbfI ScaI SexAI SgrAI
SpeI SphI Sse8387I SwaI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AclI 5119 AACGTT AA^CGTT
Alw44I 1540 GTGCAC G^TGCAC
ApaLI 1540 GTGCAC G^TGCAC
AspI 852 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 884 TGCGCA TGC^GCA
BcgI 4170 GCACGTGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 5304 GGATAC GTATCC(6/5)
BlpI 3726 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3726 GCTGAGC GC^T[ACGT]AGC
BsiWI 5006 CGTACG C^GTACG
BspLU11I 2548 ACATGT A^CATGT
BsrBI 217 GAGCGG CCGCTC(-3/-3)
BstBI 5549 TTCGAA TT^CGAA
CelII 3726 GCTGAGC GC^T[ACGT]AGC
DraIII 2752 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 4895 CGGCCG C^GGCCG
EclXI 4895 CGGCCG C^GGCCG
Eco52I 4895 CGGCCG C^GGCCG
EcoT22I 5619 ATGCAT ATGCA^T
FseI 4892 GGCCGGCC GGCCGG^CC
FspI 884 TGCGCA TGC^GCA
HincII 655 GTTGAC GT[CT]^[GA]AC
HindII 655 GTTGAC GT[CT]^[GA]AC
NheI 759 GCTAGC G^CTAGC
NsiI 5619 ATGCAT ATGCA^T
NspV 5549 TTCGAA TT^CGAA
PflFI 852 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3466 GACGAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 5119 AACGTT AA^CGTT
SapI 675 GCTCTTC GCTCTTC(1/4)
SfiI 3030 GGCCTACGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 5549 TTCGAA TT^CGAA
SnaBI 2965 TACGTA TAC^GTA
Tth111I 852 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 45 enzymes) #
###################################
AccIII 3109 TCCGGA T^CCGGA
3895 TCCGGA
AcsI 2493 AAATTC [GA]^AATT[CT]
4006 AAATTC
ApoI 2493 AAATTC [GA]^AATT[CT]
4006 AAATTC
BalI 4151 TGGCCA TGG^CCA
4283 TGGCCA
BbeI 801 GGCGCC GGCGC^C
1358 GGCGCC
BbrPI 4171 CACGTG CAC^GTG
4912 CACGTG
BbsI 3936 GAAGAC GAAGAC(2/6)
4600 GAAGAC
BbvCI 1463 CCTCAGC CCTCAGC(-5/-2)
3532 GCTGAGG
BclI 3002 TGATCA T^GATCA
3995 TGATCA
BglII 1142 AGATCT A^GATCT
2660 AGATCT
BpuAI 3936 GAAGAC GAAGAC(2/6)
4600 GAAGAC
BseAI 3109 TCCGGA T^CCGGA
3895 TCCGGA
BsiEI 4895 CGGCCG CG[GA][CT]^CG
5356 CGGTCG
BsmBI 1533 CGTCTC CGTCTC(1/5)
3543 CGTCTC
BsmI 3899 GAATGC GAATGC(1/-1)
4924 GCATTC
Bsp1407I 2801 TGTACA T^GTACA
3383 TGTACA
BspEI 3109 TCCGGA T^CCGGA
3895 TCCGGA
BspHI 3653 TCATGA T^CATGA
3752 TCATGA
BsrGI 2801 TGTACA T^GTACA
3383 TGTACA
BssHII 350 GCGCGC G^CGCGC
570 GCGCGC
BssSI 5071 CACGAG CACGAG(-5/-1)
3619 CTCGTG
Bst1107I 4561 GTATAC GTA^TAC
5632 GTATAC
BstEII 3606 GGTGACC G^GT[ACGT]ACC
5080 GGTAACC
BstPI 3606 GGTGACC G^GT[ACGT]ACC
5080 GGTAACC
BstZ17I 4561 GTATAC GTA^TAC
5632 GTATAC
DraI 3396 TTTAAA TTT^AAA
5652 TTTAAA
EcoO65I 3606 GGTGACC G^GT[ACGT]ACC
5080 GGTAACC
FbaI 3002 TGATCA T^GATCA
3995 TGATCA
HaeII 801 GGCGCC [GA]GCGC^[CT]
1358 GGCGCC
KasI 801 GGCGCC G^GCGCC
1358 GGCGCC
MluNI 4151 TGGCCA TGG^CCA
4283 TGGCCA
MroI 3109 TCCGGA T^CCGGA
3895 TCCGGA
MscI 4151 TGGCCA TGG^CCA
4283 TGGCCA
NaeI 798 GCCGGC GCC^GGC
4893 GCCGGC
NarI 801 GGCGCC GG^CGCC
1358 GGCGCC
NcoI 2568 CCATGG C^CATGG
3858 CCATGG
NgoMIV 798 GCCGGC G^CCGGC
4893 GCCGGC
PflMI 4267 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4988 CCAGCTTGTGG
PmaCI 4171 CACGTG CAC^GTG
4912 CACGTG
PmlI 4171 CACGTG CAC^GTG
4912 CACGTG
RcaI 3653 TCATGA T^CATGA
3752 TCATGA
SfoI 801 GGCGCC GGC^GCC
1358 GGCGCC
SspBI 2801 TGTACA T^GTACA
3383 TGTACA
SspI 3427 AATATT AAT^ATT
4401 AATATT
Van91I 4267 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4988 CCAGCTTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
Acc65I AccI AccII AciI AcyI
AfaI AflIII AhdI AluI AlwI
AlwNI AocI ApaI Asp718I AspEI
AspHI AvaI AvaII BamHI BanI
BanII BbvI BcnI BfaI BglI
BmyI BpmI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI Eam1105I EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KpnI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI SacI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmaCI XmaI