##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI Alw44I ApaLI AseI
AsnI Asp700I Asp718I AviII AvrII
BbrPI BcgI BclI BfrI BlnI
BsaBI BseAI BsmI BspDI BspEI
BspHI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraIII DrdI
EcoO65I EcoRI FbaI FspI HincII
HindII HindIII HpaI KpnI MamI
MfeI MroI MunI NdeI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SalI
SbfI ScaI SfiI SfuI SgrAI
SnaBI Sse8387I SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 2862 AAATTT [GA]^AATT[CT]
AhdI 1750 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 304 AGCGCT AGC^GCT
ApoI 2862 AAATTT [GA]^AATT[CT]
AscI 286 GGCGCGCC GG^CGCGCC
AspEI 1750 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 4069 GGATCC G^GATCC
BbsI 4870 GAAGAC GAAGAC(2/6)
BpuAI 4870 GAAGAC GAAGAC(2/6)
BsaAI 2370 CACGTA [CT]AC^GT[GA]
BsaWI 1082 TCCGGT [AT]^CCGG[AT]
BsiWI 782 CGTACG C^GTACG
BspLU11I 2074 ACATGT A^CATGT
BsrBI 1302 GAGCGG CCGCTC(-3/-3)
BssSI 4361 CACGAG CACGAG(-5/-1)
DraI 3285 TTTAAA TTT^AAA
Eam1105I 1750 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 304 AGCGCT AGC^GCT
EcoRV 3657 GATATC GAT^ATC
MaeII 2371 ACGT A^CGT
MluI 2318 ACGCGT A^CGCGT
SexAI 3217 ACCAGGT A^CC[AT]GGT
SpeI 2390 ACTAGT A^CTAGT
SspI 4831 AATATT AAT^ATT
XbaI 1941 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AspI 4391 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4873 GACAGTGTC
BciVI 1294 GTATCC GTATCC(6/5)
1965 GTATCC
BsiEI 20 CGGCCG CG[GA][CT]^CG
226 CGGCCG
BsmBI 635 CGTCTC CGTCTC(1/5)
3792 GAGACG
Bsp1407I 4702 TGTACA T^GTACA
4907 TGTACA
BsrGI 4702 TGTACA T^GTACA
4907 TGTACA
BssHII 97 GCGCGC G^CGCGC
287 GCGCGC
EagI 20 CGGCCG C^GGCCG
226 CGGCCG
EclXI 20 CGGCCG C^GGCCG
226 CGGCCG
Eco52I 20 CGGCCG C^GGCCG
226 CGGCCG
EcoNI 4947 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5112 CCTTGTGAAGG
EcoT22I 2346 ATGCAT ATGCA^T
4822 ATGCAT
FseI 943 GGCCGGCC GGCCGG^CC
4146 GGCCGGCC
NsiI 2346 ATGCAT ATGCA^T
4822 ATGCAT
PflFI 4391 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4873 GACAGTGTC
PshAI 596 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3003 GACACAAGTC
SacI 543 GAGCTC GAGCT^C
1701 GAGCTC
SapI 540 GAAGAGC GCTCTTC(1/4)
609 GAAGAGC
SmaI 234 CCCGGG CCC^GGG
1226 CCCGGG
SmlI 1817 CTTGAG C^T[CT][GA]AG
4329 CTTGAG
SphI 2253 GCATGC GCATG^C
2738 GCATGC
SspBI 4702 TGTACA T^GTACA
4907 TGTACA
Tth111I 4391 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4873 GACAGTGTC
XmaCI 234 CCCGGG C^CCGGG
1226 CCCGGG
XmaI 234 CCCGGG C^CCGGG
1226 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbvCI BbvI BcnI
BfaI BglI BglII BlpI BmyI
BpmI Bpu1102I BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII