################################## # NO CUT ENZYMES ( 60 enzymes) # ################################## AclI AflII AhdI Alw44I ApaLI AscI AseI AsnI AspEI AspI AvrII BbrPI BclI BfrI BlnI BsiWI Bsp1407I BspDI BsrGI Bst1107I BstBI BstZ17I ClaI CpoI DraI Eam1105I EcoT22I FbaI FseI HpaI MfeI MunI NdeI NotI NsiI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SalI SbfI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 20 enzymes) # ################################### Acc65I 2926 GGTACC G^GTACC Aor51HI 1701 AGCGCT AGC^GCT Asp718I 2926 GGTACC G^GTACC BcgI 4840 CGACATTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 3013 GTATCC GTATCC(6/5) BsaAI 5367 CACGTA [CT]AC^GT[GA] BsaBI 2274 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC Eco47III 1701 AGCGCT AGC^GCT EcoRV 2371 GATATC GAT^ATC HincII 3442 GTCAAC GT[CT]^[GA]AC HindII 3442 GTCAAC GT[CT]^[GA]AC KpnI 2926 GGTACC GGTAC^C MamI 2274 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 1814 ACGCGT A^CGCGT NaeI 1975 GCCGGC GCC^GGC NgoMIV 1975 GCCGGC G^CCGGC NheI 1852 GCTAGC G^CTAGC NruI 3676 TCGCGA TCG^CGA PshAI 559 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC ScaI 724 AGTACT AGT^ACT ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccIII 443 TCCGGA T^CCGGA 2357 TCCGGA AgeI 1931 ACCGGT A^CCGGT 3914 ACCGGT Asp700I 22 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 393 GAAGGAATTC AviII 1966 TGCGCA TGC^GCA 4239 TGCGCA BbsI 295 GTCTTC GAAGAC(2/6) 3908 GTCTTC BpuAI 295 GTCTTC GAAGAC(2/6) 3908 GTCTTC BsaI 2997 GAGACC GGTCTC(1/5) 4417 GAGACC BseAI 443 TCCGGA T^CCGGA 2357 TCCGGA BsiEI 1680 CGGCCG CG[GA][CT]^CG 1977 CGGCCG BsmI 1170 GAATGC GAATGC(1/-1) 4863 GAATGC BspEI 443 TCCGGA T^CCGGA 2357 TCCGGA BspHI 716 TCATGA T^CATGA 1226 TCATGA BspLU11I 283 ACATGT A^CATGT 3765 ACATGT BssSI 1637 CTCGTG CACGAG(-5/-1) 3084 CTCGTG DraIII 1463 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG 5537 CACTCAGTG EagI 1680 CGGCCG C^GGCCG 1977 CGGCCG EclXI 1680 CGGCCG C^GGCCG 1977 CGGCCG Eco52I 1680 CGGCCG C^GGCCG 1977 CGGCCG EcoRI 226 GAATTC G^AATTC 397 GAATTC FspI 1966 TGCGCA TGC^GCA 4239 TGCGCA HindIII 3389 AAGCTT A^AGCTT 5701 AAGCTT KspI 2061 CCGCGG CCGC^GG 2232 CCGCGG MroI 443 TCCGGA T^CCGGA 2357 TCCGGA PinAI 1931 ACCGGT A^CCGGT 3914 ACCGGT RcaI 716 TCATGA T^CATGA 1226 TCATGA SacII 2061 CCGCGG CCGC^GG 2232 CCGCGG SexAI 851 ACCTGGT A^CC[AT]GGT 4220 ACCTGGT SphI 585 GCATGC GCATG^C 1969 GCATGC XcmI 1362 CCAACATGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1656 CCAAGCGGCTCATGG XmnI 22 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 393 GAAGGAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 148 enzymes) # ######################################### AatII AccI AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApaI ApoI AspHI AvaI AvaII BalI BamHI BanI BanII BbeI BbvCI BbvI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BsaHI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssHII BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmaCI XmaI