##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AclI AflII AhdI Alw44I ApaLI
AscI AseI AsnI AspEI AspI
AvrII BbrPI BclI BfrI BlnI
BsiWI Bsp1407I BspDI BsrGI Bst1107I
BstBI BstZ17I ClaI CpoI DraI
Eam1105I EcoT22I FbaI FseI HpaI
MfeI MunI NdeI NotI NsiI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SalI SbfI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 20 enzymes) #
###################################
Acc65I 2926 GGTACC G^GTACC
Aor51HI 1701 AGCGCT AGC^GCT
Asp718I 2926 GGTACC G^GTACC
BcgI 4840 CGACATTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3013 GTATCC GTATCC(6/5)
BsaAI 5367 CACGTA [CT]AC^GT[GA]
BsaBI 2274 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Eco47III 1701 AGCGCT AGC^GCT
EcoRV 2371 GATATC GAT^ATC
HincII 3442 GTCAAC GT[CT]^[GA]AC
HindII 3442 GTCAAC GT[CT]^[GA]AC
KpnI 2926 GGTACC GGTAC^C
MamI 2274 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1814 ACGCGT A^CGCGT
NaeI 1975 GCCGGC GCC^GGC
NgoMIV 1975 GCCGGC G^CCGGC
NheI 1852 GCTAGC G^CTAGC
NruI 3676 TCGCGA TCG^CGA
PshAI 559 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 724 AGTACT AGT^ACT
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccIII 443 TCCGGA T^CCGGA
2357 TCCGGA
AgeI 1931 ACCGGT A^CCGGT
3914 ACCGGT
Asp700I 22 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
393 GAAGGAATTC
AviII 1966 TGCGCA TGC^GCA
4239 TGCGCA
BbsI 295 GTCTTC GAAGAC(2/6)
3908 GTCTTC
BpuAI 295 GTCTTC GAAGAC(2/6)
3908 GTCTTC
BsaI 2997 GAGACC GGTCTC(1/5)
4417 GAGACC
BseAI 443 TCCGGA T^CCGGA
2357 TCCGGA
BsiEI 1680 CGGCCG CG[GA][CT]^CG
1977 CGGCCG
BsmI 1170 GAATGC GAATGC(1/-1)
4863 GAATGC
BspEI 443 TCCGGA T^CCGGA
2357 TCCGGA
BspHI 716 TCATGA T^CATGA
1226 TCATGA
BspLU11I 283 ACATGT A^CATGT
3765 ACATGT
BssSI 1637 CTCGTG CACGAG(-5/-1)
3084 CTCGTG
DraIII 1463 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
5537 CACTCAGTG
EagI 1680 CGGCCG C^GGCCG
1977 CGGCCG
EclXI 1680 CGGCCG C^GGCCG
1977 CGGCCG
Eco52I 1680 CGGCCG C^GGCCG
1977 CGGCCG
EcoRI 226 GAATTC G^AATTC
397 GAATTC
FspI 1966 TGCGCA TGC^GCA
4239 TGCGCA
HindIII 3389 AAGCTT A^AGCTT
5701 AAGCTT
KspI 2061 CCGCGG CCGC^GG
2232 CCGCGG
MroI 443 TCCGGA T^CCGGA
2357 TCCGGA
PinAI 1931 ACCGGT A^CCGGT
3914 ACCGGT
RcaI 716 TCATGA T^CATGA
1226 TCATGA
SacII 2061 CCGCGG CCGC^GG
2232 CCGCGG
SexAI 851 ACCTGGT A^CC[AT]GGT
4220 ACCTGGT
SphI 585 GCATGC GCATG^C
1969 GCATGC
XcmI 1362 CCAACATGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1656 CCAAGCGGCTCATGG
XmnI 22 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
393 GAAGGAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 148 enzymes) #
#########################################
AatII AccI AccII AciI AcsI
AcyI AfaI AflIII AluI AlwI
AlwNI AocI ApaI ApoI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbeI BbvCI BbvI BcnI
BfaI BglI BglII BlpI BmyI
BpmI Bpu1102I BsaHI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssHII BssKI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmaCI XmaI