################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## AatII AccI AccIII AclI AflII AgeI AhdI Aor51HI AscI AspEI AviII AvrII BbeI BbsI BfrI BglII BlnI BpuAI BsaBI BsaWI BseAI BsiWI BspDI BspEI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoT22I FseI FspI HpaI KasI KspI MamI MroI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI Psp1406I PvuI RsrII SacII SalI SfiI SfoI SfuI SgrAI SpeI SwaI XhoI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### Acc65I 2629 GGTACC G^GTACC AcyI 2502 GACGCC G[GA]^CG[CT]C Alw44I 3032 GTGCAC G^TGCAC ApaI 2711 GGGCCC GGGCC^C ApaLI 3032 GTGCAC G^TGCAC Asp718I 2629 GGTACC G^GTACC AspI 2502 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 146 GGATCC G^GATCC BbrPI 92 CACGTG CAC^GTG BbvCI 4878 GCTGAGG CCTCAGC(-5/-2) BsaHI 2502 GACGCC G[GA]^CG[CT]C BsiEI 58 CGGTCG CG[GA][CT]^CG BspLU11I 1003 ACATGT A^CATGT BsrBI 2625 GAGCGG CCGCTC(-3/-3) BsrFI 56 GCCGGT [GA]^CCGG[CT] BstEII 1541 GGTCACC G^GT[ACGT]ACC BstPI 1541 GGTCACC G^GT[ACGT]ACC Cfr10I 56 GCCGGT [GA]^CCGG[CT] EcoO65I 1541 GGTCACC G^GT[ACGT]ACC EcoRV 1872 GATATC GAT^ATC HaeII 208 GGCGCT [GA]GCGC^[CT] Hin1I 2502 GACGCC G[GA]^CG[CT]C KpnI 2629 GGTACC GGTAC^C MfeI 1792 CAATTG C^AATTG MluI 29 ACGCGT A^CGCGT MunI 1792 CAATTG C^AATTG NdeI 1786 CATATG CA^TATG PflFI 2502 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 92 CACGTG CAC^GTG PmlI 92 CACGTG CAC^GTG PstI 363 CTGCAG CTGCA^G SacI 2964 GAGCTC GAGCT^C SapI 3925 GCTCTTC GCTCTTC(1/4) SbfI 362 CCTGCAGG CCTGCA^GG ScaI 1700 AGTACT AGT^ACT SnaBI 2030 TACGTA TAC^GTA SphI 4070 GCATGC GCATG^C Sse8387I 362 CCTGCAGG CCTGCA^GG Tth111I 2502 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 4775 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AseI 3342 ATTAAT AT^TAAT 3459 ATTAAT AsnI 3342 ATTAAT AT^TAAT 3459 ATTAAT Asp700I 167 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 291 GAAAATATTC BciVI 2915 GGATAC GTATCC(6/5) 5180 GTATCC BclI 680 TGATCA T^GATCA 3309 TGATCA BlpI 135 GCTGAGC GC^T[ACGT]AGC 2622 GCTGAGC BpmI 958 CTGGAG CTGGAG(16/14) 5650 CTGGAG Bpu1102I 135 GCTGAGC GC^T[ACGT]AGC 2622 GCTGAGC BseRI 3566 CTCCTC GAGGAG(10/8) 433 GAGGAG Bsp1407I 152 TGTACA T^GTACA 5018 TGTACA BspMI 1623 ACCTGC ACCTGC(4/8) 1856 GCAGGT BsrGI 152 TGTACA T^GTACA 5018 TGTACA CelII 135 GCTGAGC GC^T[ACGT]AGC 2622 GCTGAGC DraIII 642 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG 3035 CACTTGGTG DsaI 2312 CCATGG C^C[GA][CT]GG 5327 CCATGG FbaI 680 TGATCA T^GATCA 3309 TGATCA HindIII 2289 AAGCTT A^AGCTT 3289 AAGCTT NcoI 2312 CCATGG C^CATGG 5327 CCATGG PpuMI 4339 GGGTCCC [GA]G^G[AT]CC[CT] 5547 GGGTCCC PshBI 3342 ATTAAT AT^TAAT 3459 ATTAAT SspBI 152 TGTACA T^GTACA 5018 TGTACA XmnI 167 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 291 GAAAATATTC ######################################### # 3 OR MORE CUT ENZYMES ( 127 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI AspHI AvaI AvaII BalI BanI BanII BbvI BcgI BcnI BfaI BglI BmyI BsaAI BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspHI BsrDI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EaeI EarI Eco81I EcoNI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmaI SmlI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI