##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AhdI Aor51HI AscI AspEI
AviII AvrII BbeI BbsI BfrI
BglII BlnI BpuAI BsaBI BsaWI
BseAI BsiWI BspDI BspEI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoT22I FseI FspI HpaI
KasI KspI MamI MroI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI Psp1406I PvuI RsrII
SacII SalI SfiI SfoI SfuI
SgrAI SpeI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 2629 GGTACC G^GTACC
AcyI 2502 GACGCC G[GA]^CG[CT]C
Alw44I 3032 GTGCAC G^TGCAC
ApaI 2711 GGGCCC GGGCC^C
ApaLI 3032 GTGCAC G^TGCAC
Asp718I 2629 GGTACC G^GTACC
AspI 2502 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 146 GGATCC G^GATCC
BbrPI 92 CACGTG CAC^GTG
BbvCI 4878 GCTGAGG CCTCAGC(-5/-2)
BsaHI 2502 GACGCC G[GA]^CG[CT]C
BsiEI 58 CGGTCG CG[GA][CT]^CG
BspLU11I 1003 ACATGT A^CATGT
BsrBI 2625 GAGCGG CCGCTC(-3/-3)
BsrFI 56 GCCGGT [GA]^CCGG[CT]
BstEII 1541 GGTCACC G^GT[ACGT]ACC
BstPI 1541 GGTCACC G^GT[ACGT]ACC
Cfr10I 56 GCCGGT [GA]^CCGG[CT]
EcoO65I 1541 GGTCACC G^GT[ACGT]ACC
EcoRV 1872 GATATC GAT^ATC
HaeII 208 GGCGCT [GA]GCGC^[CT]
Hin1I 2502 GACGCC G[GA]^CG[CT]C
KpnI 2629 GGTACC GGTAC^C
MfeI 1792 CAATTG C^AATTG
MluI 29 ACGCGT A^CGCGT
MunI 1792 CAATTG C^AATTG
NdeI 1786 CATATG CA^TATG
PflFI 2502 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 92 CACGTG CAC^GTG
PmlI 92 CACGTG CAC^GTG
PstI 363 CTGCAG CTGCA^G
SacI 2964 GAGCTC GAGCT^C
SapI 3925 GCTCTTC GCTCTTC(1/4)
SbfI 362 CCTGCAGG CCTGCA^GG
ScaI 1700 AGTACT AGT^ACT
SnaBI 2030 TACGTA TAC^GTA
SphI 4070 GCATGC GCATG^C
Sse8387I 362 CCTGCAGG CCTGCA^GG
Tth111I 2502 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4775 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AseI 3342 ATTAAT AT^TAAT
3459 ATTAAT
AsnI 3342 ATTAAT AT^TAAT
3459 ATTAAT
Asp700I 167 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
291 GAAAATATTC
BciVI 2915 GGATAC GTATCC(6/5)
5180 GTATCC
BclI 680 TGATCA T^GATCA
3309 TGATCA
BlpI 135 GCTGAGC GC^T[ACGT]AGC
2622 GCTGAGC
BpmI 958 CTGGAG CTGGAG(16/14)
5650 CTGGAG
Bpu1102I 135 GCTGAGC GC^T[ACGT]AGC
2622 GCTGAGC
BseRI 3566 CTCCTC GAGGAG(10/8)
433 GAGGAG
Bsp1407I 152 TGTACA T^GTACA
5018 TGTACA
BspMI 1623 ACCTGC ACCTGC(4/8)
1856 GCAGGT
BsrGI 152 TGTACA T^GTACA
5018 TGTACA
CelII 135 GCTGAGC GC^T[ACGT]AGC
2622 GCTGAGC
DraIII 642 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
3035 CACTTGGTG
DsaI 2312 CCATGG C^C[GA][CT]GG
5327 CCATGG
FbaI 680 TGATCA T^GATCA
3309 TGATCA
HindIII 2289 AAGCTT A^AGCTT
3289 AAGCTT
NcoI 2312 CCATGG C^CATGG
5327 CCATGG
PpuMI 4339 GGGTCCC [GA]G^G[AT]CC[CT]
5547 GGGTCCC
PshBI 3342 ATTAAT AT^TAAT
3459 ATTAAT
SspBI 152 TGTACA T^GTACA
5018 TGTACA
XmnI 167 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
291 GAAAATATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbvI BcgI BcnI BfaI
BglI BmyI BsaAI BsaI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspHI BsrDI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EaeI
EarI Eco81I EcoNI EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmaI
SmlI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI