##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AgeI ApoI AscI Asp700I Asp718I
AvrII BcgI BclI BfrI BlnI
BsaBI BspHI BsrDI BssSI Bst1107I
BstBI BstZ17I CpoI DraI EcoRI
EcoRV FbaI HindIII HpaI KpnI
MamI MfeI MluI MunI NdeI
NotI NspV PacI PinAI PmeI
Psp1406I PvuI RcaI RsrII SacI
SalI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SwaI
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AatII 1734 GACGTC GACGT^C
AspI 2953 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 178 GGATCC G^GATCC
BglII 5145 AGATCT A^GATCT
BlpI 2756 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2756 GCTGAGC GC^T[ACGT]AGC
BsiWI 3913 CGTACG C^GTACG
BsmBI 528 GAGACG CGTCTC(1/5)
BsmI 4940 GCATTC GAATGC(1/-1)
Bsp1407I 3171 TGTACA T^GTACA
BspDI 3053 ATCGAT AT^CGAT
BspLU11I 4496 ACATGT A^CATGT
BsrGI 3171 TGTACA T^GTACA
CelII 2756 GCTGAGC GC^T[ACGT]AGC
ClaI 3053 ATCGAT AT^CGAT
DraIII 2921 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2264 GACAAGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 3865 ATGCAT ATGCA^T
NheI 186 GCTAGC G^CTAGC
NruI 1194 TCGCGA TCG^CGA
NsiI 3865 ATGCAT ATGCA^T
PaeR7I 2111 CTCGAG C^TCGAG
PflFI 2953 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3557 CCAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 5035 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 1830 ACCAGGT A^CC[AT]GGT
SphI 4381 GCATGC GCATG^C
SspBI 3171 TGTACA T^GTACA
SspI 4998 AATATT AAT^ATT
TfiI 1722 GAATC G^A[AT]TC
Tth111I 2953 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3557 CCAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2111 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AhdI 3095 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4357 GACATGCTGTC
AocI 449 CCTGAGG CC^T[ACGT]AGG
1798 CCTCAGG
Aor51HI 915 AGCGCT AGC^GCT
3482 AGCGCT
AseI 1750 ATTAAT AT^TAAT
5001 ATTAAT
AsnI 1750 ATTAAT AT^TAAT
5001 ATTAAT
AspEI 3095 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4357 GACATGCTGTC
BbvCI 3478 CCTCAGC CCTCAGC(-5/-2)
272 GCTGAGG
BciVI 4550 GGATAC GTATCC(6/5)
4116 GTATCC
BstAPI 331 GCAGGACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4475 GCAGATATTGC
BstEII 254 GGTCACC G^GT[ACGT]ACC
1834 GGTAACC
BstPI 254 GGTCACC G^GT[ACGT]ACC
1834 GGTAACC
BstYI 178 GGATCC [GA]^GATC[CT]
5145 AGATCT
Bsu36I 449 CCTGAGG CC^T[ACGT]AGG
1798 CCTCAGG
Eam1105I 3095 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4357 GACATGCTGTC
Eco47III 915 AGCGCT AGC^GCT
3482 AGCGCT
Eco81I 449 CCTGAGG CC^T[ACGT]AGG
1798 CCTCAGG
EcoNI 102 CCTCTGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1400 CCTGGAAGAGG
EcoO65I 254 GGTCACC G^GT[ACGT]ACC
1834 GGTAACC
FseI 466 GGCCGGCC GGCCGG^CC
3713 GGCCGGCC
HincII 1731 GTTGAC GT[CT]^[GA]AC
2495 GTCAAC
HindII 1731 GTTGAC GT[CT]^[GA]AC
2495 GTCAAC
MflI 178 GGATCC [GA]^GATC[CT]
5145 AGATCT
PshBI 1750 ATTAAT AT^TAAT
5001 ATTAAT
PvuII 931 CAGCTG CAG^CTG
1307 CAGCTG
SapI 1635 GAAGAGC GCTCTTC(1/4)
1645 GAAGAGC
Tsp509I 5155 AATT ^AATT
5193 AATT
XhoII 178 GGATCC [GA]^GATC[CT]
5145 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
ApaI ApaLI AspHI AvaI AvaII
AviII BalI BanI BanII BbeI
BbrPI BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspEI BspMI BsrBI BsrFI BsrI
BssHII BssKI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MroI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PleI PmaCI PmlI PpuMI PstI
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
StuI StyI TaqI Tru9I TseI
Tsp45I TspRI TthHB8I XcmI XmaCI
XmaI