##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII AclI Aor51HI AscI AviII
BbeI BglI BsaBI BsiEI BsiWI
BspHI BspLU11I BsrBI BssHII Bst1107I
BstAPI BstBI BstZ17I CpoI EagI
EclXI Eco47III Eco52I EcoRV FseI
FspI HpaI KasI MamI MluI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PmeI PshAI
Psp1406I PvuI RcaI RsrII SalI
SbfI SexAI SfiI SfoI SfuI
SnaBI SpeI Sse8387I SwaI XbaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 761 GGTACC G^GTACC
AccI 2221 GTCTAC GT^[AC][GT]AC
AccIII 5834 TCCGGA T^CCGGA
AcyI 417 GACGCC G[GA]^CG[CT]C
AgeI 1189 ACCGGT A^CCGGT
AhdI 1251 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 5761 GTGCAC G^TGCAC
ApaI 4039 GGGCCC GGGCC^C
ApaLI 5761 GTGCAC G^TGCAC
AseI 4813 ATTAAT AT^TAAT
AsnI 4813 ATTAAT AT^TAAT
Asp718I 761 GGTACC G^GTACC
AspEI 1251 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 6155 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 5718 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3159 TGATCA T^GATCA
BlpI 851 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 851 GCTCAGC GC^T[ACGT]AGC
BsaHI 417 GACGCC G[GA]^CG[CT]C
BseAI 5834 TCCGGA T^CCGGA
Bsp1407I 3528 TGTACA T^GTACA
BspEI 5834 TCCGGA T^CCGGA
BsrFI 1189 ACCGGT [GA]^CCGG[CT]
BsrGI 3528 TGTACA T^GTACA
BstEII 3535 GGTAACC G^GT[ACGT]ACC
BstPI 3535 GGTAACC G^GT[ACGT]ACC
CelII 851 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1189 ACCGGT [GA]^CCGG[CT]
DraIII 3953 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2212 GACTGCTGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1251 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3535 GGTAACC G^GT[ACGT]ACC
FbaI 3159 TGATCA T^GATCA
Hin1I 417 GACGCC G[GA]^CG[CT]C
HincII 1939 GTCAAC GT[CT]^[GA]AC
HindII 1939 GTCAAC GT[CT]^[GA]AC
KpnI 761 GGTACC GGTAC^C
KspI 39 CCGCGG CCGC^GG
MfeI 807 CAATTG C^AATTG
MroI 5834 TCCGGA T^CCGGA
MunI 807 CAATTG C^AATTG
NcoI 4673 CCATGG C^CATGG
NdeI 3381 CATATG CA^TATG
PaeR7I 3763 CTCGAG C^TCGAG
PflFI 6155 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1189 ACCGGT A^CCGGT
PshBI 4813 ATTAAT AT^TAAT
SacI 4835 GAGCTC GAGCT^C
SacII 39 CCGCGG CCGC^GG
ScaI 4924 AGTACT AGT^ACT
SgrAI 1188 CACCGGTG C[GA]^CCGG[CT]G
SspBI 3528 TGTACA T^GTACA
Tth111I 6155 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3763 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 40 CGCG CG^CG
52 CGCG
AflII 2537 CTTAAG C^TTAAG
4136 CTTAAG
AflIII 3005 ACGTGT A^C[GA][CT]GT
5068 ACACGT
AocI 1785 CCTCAGG CC^T[ACGT]AGG
5532 CCTGAGG
AvrII 283 CCTAGG C^CTAGG
685 CCTAGG
BciVI 709 GTATCC GTATCC(6/5)
961 GTATCC
BfrI 2537 CTTAAG C^TTAAG
4136 CTTAAG
BlnI 283 CCTAGG C^CTAGG
685 CCTAGG
BsaWI 1189 ACCGGT [AT]^CCGG[AT]
5834 TCCGGA
BsmBI 5071 CGTCTC CGTCTC(1/5)
5409 CGTCTC
BspDI 3611 ATCGAT AT^CGAT
4011 ATCGAT
BsrDI 1518 CATTGC GCAATG(2/0)
1109 GCAATG
BssSI 5119 CTCGTG CACGAG(-5/-1)
5736 CTCGTG
BstUI 40 CGCG CG^CG
52 CGCG
Bsu36I 1785 CCTCAGG CC^T[ACGT]AGG
5532 CCTGAGG
ClaI 3611 ATCGAT AT^CGAT
4011 ATCGAT
Eco81I 1785 CCTCAGG CC^T[ACGT]AGG
5532 CCTGAGG
EcoRI 1676 GAATTC G^AATTC
2012 GAATTC
EcoT22I 3378 ATGCAT ATGCA^T
4994 ATGCAT
HaeII 1 GGCGCT [GA]GCGC^[CT]
5543 GGCGCT
HindIII 1666 AAGCTT A^AGCTT
4115 AAGCTT
MvnI 40 CGCG CG^CG
52 CGCG
NsiI 3378 ATGCAT ATGCA^T
4994 ATGCAT
SmaI 4624 CCCGGG CCC^GGG
5706 CCCGGG
SphI 306 GCATGC GCATG^C
4656 GCATGC
SspI 2031 AATATT AAT^ATT
3504 AATATT
StuI 118 AGGCCT AGG^CCT
5536 AGGCCT
XmaCI 4624 CCCGGG C^CCGGG
5706 CCCGGG
XmaI 4624 CCCGGG C^CCGGG
5706 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaI
AvaII BalI BamHI BanI BanII
BbrPI BbsI BbvCI BbvI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmnI