##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AccI AflII Alw44I ApaLI
AscI Asp700I AvrII BbrPI BciVI
BfrI BglII BlnI BsiWI BsmI
BspDI BsrDI BssHII Bst1107I BstBI
BstXI BstZ17I ClaI CpoI DrdI
EcoRV EcoT22I FseI HindIII HpaI
MfeI MluI MunI NaeI NdeI
NgoMIV NheI NotI NsiI NspV
PacI PmaCI PmeI PmlI PshAI
PstI PvuI RsrII SalI SapI
SbfI ScaI SexAI SfuI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
Acc65I 2789 GGTACC G^GTACC
AccIII 100 TCCGGA T^CCGGA
AclI 4364 AACGTT AA^CGTT
AgeI 3202 ACCGGT A^CCGGT
AhdI 1299 GACTTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1466 AGCGCT AGC^GCT
AseI 1576 ATTAAT AT^TAAT
AsnI 1576 ATTAAT AT^TAAT
Asp718I 2789 GGTACC G^GTACC
AspEI 1299 GACTTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 637 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 518 GGCGCC GGCGC^C
BclI 3006 TGATCA T^GATCA
BglI 291 GCCTCTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 506 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 506 GCTGAGC GC^T[ACGT]AGC
BsaBI 1060 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 100 TCCGGA T^CCGGA
BsiEI 3826 CGGCCG CG[GA][CT]^CG
Bsp1407I 2915 TGTACA T^GTACA
BspEI 100 TCCGGA T^CCGGA
BspHI 3694 TCATGA T^CATGA
BspLU11I 624 ACATGT A^CATGT
BsrFI 3202 ACCGGT [GA]^CCGG[CT]
BsrGI 2915 TGTACA T^GTACA
CelII 506 GCTGAGC GC^T[ACGT]AGC
Cfr10I 3202 ACCGGT [GA]^CCGG[CT]
DraIII 389 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 3826 CGGCCG C^GGCCG
Eam1105I 1299 GACTTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3826 CGGCCG C^GGCCG
Eco47III 1466 AGCGCT AGC^GCT
Eco52I 3826 CGGCCG C^GGCCG
EcoNI 3353 CCTATGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 3006 TGATCA T^GATCA
HincII 716 GTCAAC GT[CT]^[GA]AC
HindII 716 GTCAAC GT[CT]^[GA]AC
KasI 518 GGCGCC G^GCGCC
KpnI 2789 GGTACC GGTAC^C
KspI 3979 CCGCGG CCGC^GG
MamI 1060 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 100 TCCGGA T^CCGGA
NarI 518 GGCGCC GG^CGCC
NruI 1293 TCGCGA TCG^CGA
PaeR7I 281 CTCGAG C^TCGAG
PflFI 637 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 633 CCAAGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 3202 ACCGGT A^CCGGT
PshBI 1576 ATTAAT AT^TAAT
Psp1406I 4364 AACGTT AA^CGTT
PvuII 1107 CAGCTG CAG^CTG
RcaI 3694 TCATGA T^CATGA
SacII 3979 CCGCGG CCGC^GG
SfcI 1201 CTATAG C^T[GA][CT]AG
SfiI 290 GGCCTCTCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 518 GGCGCC GGC^GCC
SspBI 2915 TGTACA T^GTACA
Tth111I 637 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 633 CCAAGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 281 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcyI 518 GGCGCC G[GA]^CG[CT]C
3319 GGCGTC
AflIII 624 ACATGT A^C[GA][CT]GT
3650 ACACGT
AocI 2603 CCTCAGG CC^T[ACGT]AGG
3286 CCTCAGG
AviII 195 TGCGCA TGC^GCA
366 TGCGCA
BamHI 1096 GGATCC G^GATCC
3084 GGATCC
BcgI 3348 CGATTCCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3330 GCACGATTCTCG
BsaAI 149 TACGTG [CT]AC^GT[GA]
3651 CACGTA
BsaHI 518 GGCGCC G[GA]^CG[CT]C
3319 GGCGTC
BsaWI 100 TCCGGA [AT]^CCGG[AT]
3202 ACCGGT
BsmBI 3624 CGTCTC CGTCTC(1/5)
128 GAGACG
BsrBI 981 GAGCGG CCGCTC(-3/-3)
1371 GAGCGG
BssSI 1121 CACGAG CACGAG(-5/-1)
1718 CACGAG
Bsu36I 2603 CCTCAGG CC^T[ACGT]AGG
3286 CCTCAGG
Eco81I 2603 CCTCAGG CC^T[ACGT]AGG
3286 CCTCAGG
FspI 195 TGCGCA TGC^GCA
366 TGCGCA
Hin1I 518 GGCGCC G[GA]^CG[CT]C
3319 GGCGTC
NcoI 747 CCATGG C^CATGG
912 CCATGG
PpuMI 2211 GGGACCT [GA]G^G[AT]CC[CT]
3243 GGGTCCC
SmlI 281 CTCGAG C^T[CT][GA]AG
3164 CTCAAG
StuI 75 AGGCCT AGG^CCT
2607 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoO65I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SmaI SphI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmaCI
XmaI