##################################
# NO CUT ENZYMES (   76 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AflIII      AhdI        ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AviII       AvrII       BcgI        BciVI       BclI        
BfrI        BglII       BlnI        BsaBI       BseAI       
BsiWI       BsmBI       BsmI        BspDI       BspEI       
BspHI       BspLU11I    BsrDI       Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     ClaI        CpoI        
DraI        DrdI        Eam1105I    EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FspI        HindIII     
HpaI        KpnI        MamI        MluI        MroI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PmeI        PshBI       Psp1406I    
PvuI        RcaI        RsrII       SalI        ScaI        
SfuI        SnaBI       SpeI        SwaI        XbaI        
XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AgeI          840  ACCGGT               A^CCGGT
Aor51HI      2517  AGCGCT               AGC^GCT
AspI         2404  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI         596  GGATCC               G^GATCC
BsaWI         840  ACCGGT               [AT]^CCGG[AT]
Bsp1407I     2870  TGTACA               T^GTACA
BsrGI        2870  TGTACA               T^GTACA
BssSI        2968  CACGAG               CACGAG(-5/-1)
BstYI         596  GGATCC               [GA]^GATC[CT]
Eco47III     2517  AGCGCT               AGC^GCT
HincII       2086  GTTGAC               GT[CT]^[GA]AC
HindII       2086  GTTGAC               GT[CT]^[GA]AC
MfeI         4358  CAATTG               C^AATTG
MflI          596  GGATCC               [GA]^GATC[CT]
MseI         4310  TTAA                 T^TAA
MunI         4358  CAATTG               C^AATTG
NcoI         3472  CCATGG               C^CATGG
PaeR7I       2877  CTCGAG               C^TCGAG
PflFI        2404  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI         840  ACCGGT               A^CCGGT
PshAI        1259  GACGGGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI         2556  GCTCTTC              GCTCTTC(1/4)
SbfI         1701  CCTGCAGG             CCTGCA^GG
SfaNI        2615  GCATC                GCATC(5/9)
Sse8387I     1701  CCTGCAGG             CCTGCA^GG
SspBI        2870  TGTACA               T^GTACA
SspI         4303  AATATT               AAT^ATT
Tru9I        4310  TTAA                 T^TAA
Tsp509I      4359  AATT                 ^AATT
Tth111I      2404  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         2877  CTCGAG               C^TCGAG
XhoII         596  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AatII        2716  GACGTC               GACGT^C
             4140  GACGTC              
BbsI         1642  GTCTTC               GAAGAC(2/6)
             2383  GTCTTC              
BpuAI        1642  GTCTTC               GAAGAC(2/6)
             2383  GTCTTC              
BssHII       2498  GCGCGC               G^CGCGC
             2688  GCGCGC              
DraIII        649  CACGTCGTG            CAC[ACGT][ACGT][ACGT]^GTG
             4076  CACATCGTG           
EcoNI         243  CCTGGGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3339  CCTGGGGCAGG         
FseI          120  GGCCGGCC             GGCCGG^CC
             4194  GGCCGGCC            
KspI         1577  CCGCGG               CCGC^GG
             2955  CCGCGG              
SacI         2353  GAGCTC               GAGCT^C
             3675  GAGCTC              
SacII        1577  CCGCGG               CCGC^GG
             2955  CCGCGG              
SfiI         1272  GGCCCAGAAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             2324  GGCCACACTGGCC       
SgrAI         839  CACCGGTG             C[GA]^CCGG[CT]G
             2022  CACCGGCG            
SmlI         2877  CTCGAG               C^T[CT][GA]AG
             3415  CTTGAG              
SphI         1436  GCATGC               GCATG^C
             2217  GCATGC              


#########################################
# 3 OR MORE CUT ENZYMES (  136 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
Alw44I      AlwI        AlwNI       AocI        ApaI        
ApaLI       AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbeI        BbrPI       BbvCI       
BbvI        BcnI        BfaI        BglI        BlpI        
BmyI        BpmI        Bpu1102I    BsaAI       BsaHI       
BsaI        BsaJI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrBI       BsrFI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstUI       BstXI       Bsu36I      Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EagI        EarI        
EclXI       Eco52I      Eco81I      EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
KasI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MluNI       MnlI        MscI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NdeII       
NgoMIV      NlaIII      NlaIV       NspI        PflMI       
PleI        PmaCI       PmlI        PpuMI       PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SexAI       SfcI        SfoI        SmaI        StuI        
StyI        TaqI        TfiI        TseI        Tsp45I      
TspRI       TthHB8I     Van91I      XcmI        XmaCI       
XmaI