################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AflIII AhdI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BcgI BciVI BclI BfrI BglII BlnI BsaBI BseAI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrDI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DrdI Eam1105I EcoO65I EcoRI EcoRV EcoT22I FbaI FspI HindIII HpaI KpnI MamI MluI MroI NdeI NheI NotI NruI NsiI NspV PacI PmeI PshBI Psp1406I PvuI RcaI RsrII SalI ScaI SfuI SnaBI SpeI SwaI XbaI XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AgeI 840 ACCGGT A^CCGGT Aor51HI 2517 AGCGCT AGC^GCT AspI 2404 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 596 GGATCC G^GATCC BsaWI 840 ACCGGT [AT]^CCGG[AT] Bsp1407I 2870 TGTACA T^GTACA BsrGI 2870 TGTACA T^GTACA BssSI 2968 CACGAG CACGAG(-5/-1) BstYI 596 GGATCC [GA]^GATC[CT] Eco47III 2517 AGCGCT AGC^GCT HincII 2086 GTTGAC GT[CT]^[GA]AC HindII 2086 GTTGAC GT[CT]^[GA]AC MfeI 4358 CAATTG C^AATTG MflI 596 GGATCC [GA]^GATC[CT] MseI 4310 TTAA T^TAA MunI 4358 CAATTG C^AATTG NcoI 3472 CCATGG C^CATGG PaeR7I 2877 CTCGAG C^TCGAG PflFI 2404 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 840 ACCGGT A^CCGGT PshAI 1259 GACGGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 2556 GCTCTTC GCTCTTC(1/4) SbfI 1701 CCTGCAGG CCTGCA^GG SfaNI 2615 GCATC GCATC(5/9) Sse8387I 1701 CCTGCAGG CCTGCA^GG SspBI 2870 TGTACA T^GTACA SspI 4303 AATATT AAT^ATT Tru9I 4310 TTAA T^TAA Tsp509I 4359 AATT ^AATT Tth111I 2404 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 2877 CTCGAG C^TCGAG XhoII 596 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AatII 2716 GACGTC GACGT^C 4140 GACGTC BbsI 1642 GTCTTC GAAGAC(2/6) 2383 GTCTTC BpuAI 1642 GTCTTC GAAGAC(2/6) 2383 GTCTTC BssHII 2498 GCGCGC G^CGCGC 2688 GCGCGC DraIII 649 CACGTCGTG CAC[ACGT][ACGT][ACGT]^GTG 4076 CACATCGTG EcoNI 243 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3339 CCTGGGGCAGG FseI 120 GGCCGGCC GGCCGG^CC 4194 GGCCGGCC KspI 1577 CCGCGG CCGC^GG 2955 CCGCGG SacI 2353 GAGCTC GAGCT^C 3675 GAGCTC SacII 1577 CCGCGG CCGC^GG 2955 CCGCGG SfiI 1272 GGCCCAGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 2324 GGCCACACTGGCC SgrAI 839 CACCGGTG C[GA]^CCGG[CT]G 2022 CACCGGCG SmlI 2877 CTCGAG C^T[CT][GA]AG 3415 CTTGAG SphI 1436 GCATGC GCATG^C 2217 GCATGC ######################################### # 3 OR MORE CUT ENZYMES ( 136 enzymes) # ######################################### AccII AciI AcyI AfaI AluI Alw44I AlwI AlwNI AocI ApaI ApaLI AspHI AvaI AvaII BalI BanI BanII BbeI BbrPI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SexAI SfcI SfoI SmaI StuI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I Van91I XcmI XmaCI XmaI