##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AhdI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BcgI BciVI BclI
BfrI BglII BlnI BsaBI BseAI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BsrDI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DrdI Eam1105I EcoO65I EcoRI
EcoRV EcoT22I FbaI FspI HindIII
HpaI KpnI MamI MluI MroI
NdeI NheI NotI NruI NsiI
NspV PacI PmeI PshBI Psp1406I
PvuI RcaI RsrII SalI ScaI
SfuI SnaBI SpeI SwaI XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AgeI 840 ACCGGT A^CCGGT
Aor51HI 2517 AGCGCT AGC^GCT
AspI 2404 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 596 GGATCC G^GATCC
BsaWI 840 ACCGGT [AT]^CCGG[AT]
Bsp1407I 2870 TGTACA T^GTACA
BsrGI 2870 TGTACA T^GTACA
BssSI 2968 CACGAG CACGAG(-5/-1)
BstYI 596 GGATCC [GA]^GATC[CT]
Eco47III 2517 AGCGCT AGC^GCT
HincII 2086 GTTGAC GT[CT]^[GA]AC
HindII 2086 GTTGAC GT[CT]^[GA]AC
MfeI 4358 CAATTG C^AATTG
MflI 596 GGATCC [GA]^GATC[CT]
MseI 4310 TTAA T^TAA
MunI 4358 CAATTG C^AATTG
NcoI 3472 CCATGG C^CATGG
PaeR7I 2877 CTCGAG C^TCGAG
PflFI 2404 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 840 ACCGGT A^CCGGT
PshAI 1259 GACGGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 2556 GCTCTTC GCTCTTC(1/4)
SbfI 1701 CCTGCAGG CCTGCA^GG
SfaNI 2615 GCATC GCATC(5/9)
Sse8387I 1701 CCTGCAGG CCTGCA^GG
SspBI 2870 TGTACA T^GTACA
SspI 4303 AATATT AAT^ATT
Tru9I 4310 TTAA T^TAA
Tsp509I 4359 AATT ^AATT
Tth111I 2404 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2877 CTCGAG C^TCGAG
XhoII 596 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AatII 2716 GACGTC GACGT^C
4140 GACGTC
BbsI 1642 GTCTTC GAAGAC(2/6)
2383 GTCTTC
BpuAI 1642 GTCTTC GAAGAC(2/6)
2383 GTCTTC
BssHII 2498 GCGCGC G^CGCGC
2688 GCGCGC
DraIII 649 CACGTCGTG CAC[ACGT][ACGT][ACGT]^GTG
4076 CACATCGTG
EcoNI 243 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3339 CCTGGGGCAGG
FseI 120 GGCCGGCC GGCCGG^CC
4194 GGCCGGCC
KspI 1577 CCGCGG CCGC^GG
2955 CCGCGG
SacI 2353 GAGCTC GAGCT^C
3675 GAGCTC
SacII 1577 CCGCGG CCGC^GG
2955 CCGCGG
SfiI 1272 GGCCCAGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2324 GGCCACACTGGCC
SgrAI 839 CACCGGTG C[GA]^CCGG[CT]G
2022 CACCGGCG
SmlI 2877 CTCGAG C^T[CT][GA]AG
3415 CTTGAG
SphI 1436 GCATGC GCATG^C
2217 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI AspHI AvaI AvaII BalI
BanI BanII BbeI BbrPI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SexAI SfcI SfoI SmaI StuI
StyI TaqI TfiI TseI Tsp45I
TspRI TthHB8I Van91I XcmI XmaCI
XmaI