##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Alw44I ApaLI AscI AseI AsnI
Asp718I AspI AviII BamHI BbrPI
BbvCI BsaAI BsaWI BseAI BsiWI
Bsp1407I BspEI BspLU11I BsrGI BssHII
BssSI BstBI BstEII BstPI CpoI
DraIII DrdI EcoO65I EcoRV FspI
HindIII HpaI KpnI MfeI MluI
MroI MunI NheI NotI NruI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacI SbfI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AflII 4730 CTTAAG C^TTAAG
AflIII 422 ACGTGT A^C[GA][CT]GT
AocI 980 CCTCAGG CC^T[ACGT]AGG
Aor51HI 32 AGCGCT AGC^GCT
ApaI 392 GGGCCC GGGCC^C
AspHI 3047 GAGCAC G[AT]GC[AT]^C
AvrII 673 CCTAGG C^CTAGG
BalI 941 TGGCCA TGG^CCA
BbeI 286 GGCGCC GGCGC^C
BcgI 3178 CGAGTCTTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 918 GTATCC GTATCC(6/5)
BclI 597 TGATCA T^GATCA
BfrI 4730 CTTAAG C^TTAAG
BglII 3564 AGATCT A^GATCT
BlnI 673 CCTAGG C^CTAGG
BlpI 93 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 93 GCTCAGC GC^T[ACGT]AGC
BsaBI 598 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 4282 CTGCAC GTGCAG(16/14)
BsiHKAI 3047 GAGCAC G[AT]GC[AT]^C
BsmBI 116 CGTCTC CGTCTC(1/5)
BspDI 1056 ATCGAT AT^CGAT
BsrFI 247 GCCGGC [GA]^CCGG[CT]
Bst1107I 3619 GTATAC GTA^TAC
BstAPI 4275 GCAGTATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 3113 CCAAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3619 GTATAC GTA^TAC
Bsu36I 980 CCTCAGG CC^T[ACGT]AGG
CelII 93 GCTCAGC GC^T[ACGT]AGC
Cfr10I 247 GCCGGC [GA]^CCGG[CT]
ClaI 1056 ATCGAT AT^CGAT
Eco47III 32 AGCGCT AGC^GCT
Eco81I 980 CCTCAGG CC^T[ACGT]AGG
EcoRI 3829 GAATTC G^AATTC
EcoT22I 4603 ATGCAT ATGCA^T
FbaI 597 TGATCA T^GATCA
FseI 246 GGCCGGCC GGCCGG^CC
HphI 59 TCACC GGTGA(8/7)
KasI 286 GGCGCC G^GCGCC
KspI 398 CCGCGG CCGC^GG
MamI 598 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 941 TGGCCA TGG^CCA
MscI 941 TGGCCA TGG^CCA
NaeI 247 GCCGGC GCC^GGC
NarI 286 GGCGCC GG^CGCC
NgoMIV 247 GCCGGC G^CCGGC
NsiI 4603 ATGCAT ATGCA^T
PpuMI 3518 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 4585 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 398 CCGCGG CCGC^GG
SalI 2699 GTCGAC G^TCGAC
SapI 2785 GAAGAGC GCTCTTC(1/4)
ScaI 2758 AGTACT AGT^ACT
SfoI 286 GGCGCC GGC^GCC
SspI 4333 AATATT AAT^ATT
StuI 3530 AGGCCT AGG^CCT
XbaI 4131 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AhdI 826 GACATGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4372 GACAACCAGTC
AspEI 826 GACATGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4372 GACAACCAGTC
BanI 286 GGCGCC G^G[CT][GA]CC
1455 GGTGCC
BanII 135 GAGCCC G[GA]GC[CT]^C
392 GGGCCC
BbsI 524 GTCTTC GAAGAC(2/6)
4852 GTCTTC
BpuAI 524 GTCTTC GAAGAC(2/6)
4852 GTCTTC
BsiEI 154 CGGCCG CG[GA][CT]^CG
245 CGGCCG
BsmI 570 GAATGC GAATGC(1/-1)
582 GAATGC
BspHI 2805 TCATGA T^CATGA
3767 TCATGA
BstYI 882 GGATCT [GA]^GATC[CT]
3564 AGATCT
EagI 154 CGGCCG C^GGCCG
245 CGGCCG
Eam1105I 826 GACATGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4372 GACAACCAGTC
EclXI 154 CGGCCG C^GGCCG
245 CGGCCG
Eco52I 154 CGGCCG C^GGCCG
245 CGGCCG
HaeII 32 AGCGCT [GA]GCGC^[CT]
286 GGCGCC
HgaI 385 GCGTC GACGC(5/10)
1125 GCGTC
MaeII 422 ACGT A^CGT
4185 ACGT
MflI 882 GGATCT [GA]^GATC[CT]
3564 AGATCT
NcoI 518 CCATGG C^CATGG
689 CCATGG
NdeI 3643 CATATG CA^TATG
4404 CATATG
PaeR7I 730 CTCGAG C^TCGAG
3350 CTCGAG
PstI 623 CTGCAG CTGCA^G
1534 CTGCAG
RcaI 2805 TCATGA T^CATGA
3767 TCATGA
SexAI 2433 ACCTGGT A^CC[AT]GGT
3623 ACCAGGT
SmaI 299 CCCGGG CCC^GGG
1708 CCCGGG
XhoI 730 CTCGAG C^TCGAG
3350 CTCGAG
XhoII 882 GGATCT [GA]^GATC[CT]
3564 AGATCT
XmaCI 299 CCCGGG C^CCGGG
1708 CCCGGG
XmaI 299 CCCGGG C^CCGGG
1708 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
Asp700I AvaI AvaII BbvI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI Hin1I HinP1I HincII
HindII HinfI HpaII ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XmnI