################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## AatII Acc65I AccIII AclI AgeI Alw44I ApaLI AscI AseI AsnI Asp718I AspI AviII BamHI BbrPI BbvCI BsaAI BsaWI BseAI BsiWI Bsp1407I BspEI BspLU11I BsrGI BssHII BssSI BstBI BstEII BstPI CpoI DraIII DrdI EcoO65I EcoRV FspI HindIII HpaI KpnI MfeI MluI MroI MunI NheI NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacI SbfI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AflII 4730 CTTAAG C^TTAAG AflIII 422 ACGTGT A^C[GA][CT]GT AocI 980 CCTCAGG CC^T[ACGT]AGG Aor51HI 32 AGCGCT AGC^GCT ApaI 392 GGGCCC GGGCC^C AspHI 3047 GAGCAC G[AT]GC[AT]^C AvrII 673 CCTAGG C^CTAGG BalI 941 TGGCCA TGG^CCA BbeI 286 GGCGCC GGCGC^C BcgI 3178 CGAGTCTTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 918 GTATCC GTATCC(6/5) BclI 597 TGATCA T^GATCA BfrI 4730 CTTAAG C^TTAAG BglII 3564 AGATCT A^GATCT BlnI 673 CCTAGG C^CTAGG BlpI 93 GCTCAGC GC^T[ACGT]AGC Bpu1102I 93 GCTCAGC GC^T[ACGT]AGC BsaBI 598 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 4282 CTGCAC GTGCAG(16/14) BsiHKAI 3047 GAGCAC G[AT]GC[AT]^C BsmBI 116 CGTCTC CGTCTC(1/5) BspDI 1056 ATCGAT AT^CGAT BsrFI 247 GCCGGC [GA]^CCGG[CT] Bst1107I 3619 GTATAC GTA^TAC BstAPI 4275 GCAGTATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 3113 CCAAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 3619 GTATAC GTA^TAC Bsu36I 980 CCTCAGG CC^T[ACGT]AGG CelII 93 GCTCAGC GC^T[ACGT]AGC Cfr10I 247 GCCGGC [GA]^CCGG[CT] ClaI 1056 ATCGAT AT^CGAT Eco47III 32 AGCGCT AGC^GCT Eco81I 980 CCTCAGG CC^T[ACGT]AGG EcoRI 3829 GAATTC G^AATTC EcoT22I 4603 ATGCAT ATGCA^T FbaI 597 TGATCA T^GATCA FseI 246 GGCCGGCC GGCCGG^CC HphI 59 TCACC GGTGA(8/7) KasI 286 GGCGCC G^GCGCC KspI 398 CCGCGG CCGC^GG MamI 598 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 941 TGGCCA TGG^CCA MscI 941 TGGCCA TGG^CCA NaeI 247 GCCGGC GCC^GGC NarI 286 GGCGCC GG^CGCC NgoMIV 247 GCCGGC G^CCGGC NsiI 4603 ATGCAT ATGCA^T PpuMI 3518 AGGACCT [GA]G^G[AT]CC[CT] PshAI 4585 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 398 CCGCGG CCGC^GG SalI 2699 GTCGAC G^TCGAC SapI 2785 GAAGAGC GCTCTTC(1/4) ScaI 2758 AGTACT AGT^ACT SfoI 286 GGCGCC GGC^GCC SspI 4333 AATATT AAT^ATT StuI 3530 AGGCCT AGG^CCT XbaI 4131 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AhdI 826 GACATGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4372 GACAACCAGTC AspEI 826 GACATGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4372 GACAACCAGTC BanI 286 GGCGCC G^G[CT][GA]CC 1455 GGTGCC BanII 135 GAGCCC G[GA]GC[CT]^C 392 GGGCCC BbsI 524 GTCTTC GAAGAC(2/6) 4852 GTCTTC BpuAI 524 GTCTTC GAAGAC(2/6) 4852 GTCTTC BsiEI 154 CGGCCG CG[GA][CT]^CG 245 CGGCCG BsmI 570 GAATGC GAATGC(1/-1) 582 GAATGC BspHI 2805 TCATGA T^CATGA 3767 TCATGA BstYI 882 GGATCT [GA]^GATC[CT] 3564 AGATCT EagI 154 CGGCCG C^GGCCG 245 CGGCCG Eam1105I 826 GACATGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4372 GACAACCAGTC EclXI 154 CGGCCG C^GGCCG 245 CGGCCG Eco52I 154 CGGCCG C^GGCCG 245 CGGCCG HaeII 32 AGCGCT [GA]GCGC^[CT] 286 GGCGCC HgaI 385 GCGTC GACGC(5/10) 1125 GCGTC MaeII 422 ACGT A^CGT 4185 ACGT MflI 882 GGATCT [GA]^GATC[CT] 3564 AGATCT NcoI 518 CCATGG C^CATGG 689 CCATGG NdeI 3643 CATATG CA^TATG 4404 CATATG PaeR7I 730 CTCGAG C^TCGAG 3350 CTCGAG PstI 623 CTGCAG CTGCA^G 1534 CTGCAG RcaI 2805 TCATGA T^CATGA 3767 TCATGA SexAI 2433 ACCTGGT A^CC[AT]GGT 3623 ACCAGGT SmaI 299 CCCGGG CCC^GGG 1708 CCCGGG XhoI 730 CTCGAG C^TCGAG 3350 CTCGAG XhoII 882 GGATCT [GA]^GATC[CT] 3564 AGATCT XmaCI 299 CCCGGG C^CCGGG 1708 CCCGGG XmaI 299 CCCGGG C^CCGGG 1708 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 104 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI Asp700I AvaI AvaII BbvI BcnI BfaI BglI BmyI BpmI BsaHI BsaI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HincII HindII HinfI HpaII ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XmnI