##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AccI AccIII AclI AgeI AhdI
Aor51HI AscI AseI AsnI AspEI
AvrII BamHI BclI BlnI BsaBI
BsaWI BseAI BsiWI BsmI BspDI
BspEI BsrFI BssHII Bst1107I BstZ17I
Cfr10I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV EcoT22I FbaI FseI HgaI
HindIII HpaI MamI MfeI MluI
MroI MunI NaeI NgoMIV NotI
NruI NsiI PacI PaeR7I PinAI
PmeI PshBI Psp1406I RsrII SalI
SbfI ScaI SfiI SgrAI SnaBI
SpeI Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AatII 1795 GACGTC GACGT^C
AviII 779 TGCGCA TGC^GCA
BbrPI 1369 CACGTG CAC^GTG
BciVI 2966 GTATCC GTATCC(6/5)
BsiEI 2079 CGATCG CG[GA][CT]^CG
BspHI 2099 TCATGA T^CATGA
BspLU11I 722 ACATGT A^CATGT
BstBI 4146 TTCGAA TT^CGAA
EcoRI 3070 GAATTC G^AATTC
FspI 779 TGCGCA TGC^GCA
HincII 210 GTTGAC GT[CT]^[GA]AC
HindII 210 GTTGAC GT[CT]^[GA]AC
KspI 4076 CCGCGG CCGC^GG
NdeI 2060 CATATG CA^TATG
NheI 1038 GCTAGC G^CTAGC
NspV 4146 TTCGAA TT^CGAA
PmaCI 1369 CACGTG CAC^GTG
PmlI 1369 CACGTG CAC^GTG
PshAI 225 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 2079 CGATCG CGAT^CG
RcaI 2099 TCATGA T^CATGA
SacII 4076 CCGCGG CCGC^GG
SapI 2616 GAAGAGC GCTCTTC(1/4)
SfuI 4146 TTCGAA TT^CGAA
SmaI 1992 CCCGGG CCC^GGG
SphI 1556 GCATGC GCATG^C
SspI 4278 AATATT AAT^ATT
XbaI 3018 TCTAGA T^CTAGA
XmaCI 1992 CCCGGG C^CCGGG
XmaI 1992 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 3011 GGTACC G^GTACC
3703 GGTACC
AflII 697 CTTAAG C^TTAAG
3785 CTTAAG
Alw44I 1647 GTGCAC G^TGCAC
3441 GTGCAC
ApaLI 1647 GTGCAC G^TGCAC
3441 GTGCAC
Asp718I 3011 GGTACC G^GTACC
3703 GGTACC
BbeI 2306 GGCGCC GGCGC^C
2898 GGCGCC
BfrI 697 CTTAAG C^TTAAG
3785 CTTAAG
BglI 604 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1172 GCCACGTAGGC
BglII 872 AGATCT A^GATCT
3214 AGATCT
BsaAI 1174 CACGTA [CT]AC^GT[GA]
1369 CACGTG
BsaI 4356 GAGACC GGTCTC(1/5)
3510 GGTCTC
BsmBI 568 CGTCTC CGTCTC(1/5)
3479 GAGACG
Bsp1407I 1688 TGTACA T^GTACA
4250 TGTACA
BsrDI 654 CATTGC GCAATG(2/0)
4067 CATTGC
BsrGI 1688 TGTACA T^GTACA
4250 TGTACA
BssSI 979 CACGAG CACGAG(-5/-1)
2947 CTCGTG
BstEII 1056 GGTCACC G^GT[ACGT]ACC
2908 GGTCACC
BstPI 1056 GGTCACC G^GT[ACGT]ACC
2908 GGTCACC
BstXI 3554 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3794 CCAAAGGCCTGG
EcoO65I 1056 GGTCACC G^GT[ACGT]ACC
2908 GGTCACC
KasI 2306 GGCGCC G^GCGCC
2898 GGCGCC
KpnI 3011 GGTACC GGTAC^C
3703 GGTACC
NarI 2306 GGCGCC GG^CGCC
2898 GGCGCC
NcoI 227 CCATGG C^CATGG
3930 CCATGG
SexAI 989 ACCTGGT A^CC[AT]GGT
2912 ACCTGGT
SfoI 2306 GGCGCC GGC^GCC
2898 GGCGCC
SspBI 1688 TGTACA T^GTACA
4250 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI Asp700I AspHI AspI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcgI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmnI