################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## AccI AccIII AclI AgeI AhdI Aor51HI AscI AseI AsnI AspEI AvrII BamHI BclI BlnI BsaBI BsaWI BseAI BsiWI BsmI BspDI BspEI BsrFI BssHII Bst1107I BstZ17I Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRV EcoT22I FbaI FseI HgaI HindIII HpaI MamI MfeI MluI MroI MunI NaeI NgoMIV NotI NruI NsiI PacI PaeR7I PinAI PmeI PshBI Psp1406I RsrII SalI SbfI ScaI SfiI SgrAI SnaBI SpeI Sse8387I SwaI XhoI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AatII 1795 GACGTC GACGT^C AviII 779 TGCGCA TGC^GCA BbrPI 1369 CACGTG CAC^GTG BciVI 2966 GTATCC GTATCC(6/5) BsiEI 2079 CGATCG CG[GA][CT]^CG BspHI 2099 TCATGA T^CATGA BspLU11I 722 ACATGT A^CATGT BstBI 4146 TTCGAA TT^CGAA EcoRI 3070 GAATTC G^AATTC FspI 779 TGCGCA TGC^GCA HincII 210 GTTGAC GT[CT]^[GA]AC HindII 210 GTTGAC GT[CT]^[GA]AC KspI 4076 CCGCGG CCGC^GG NdeI 2060 CATATG CA^TATG NheI 1038 GCTAGC G^CTAGC NspV 4146 TTCGAA TT^CGAA PmaCI 1369 CACGTG CAC^GTG PmlI 1369 CACGTG CAC^GTG PshAI 225 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 2079 CGATCG CGAT^CG RcaI 2099 TCATGA T^CATGA SacII 4076 CCGCGG CCGC^GG SapI 2616 GAAGAGC GCTCTTC(1/4) SfuI 4146 TTCGAA TT^CGAA SmaI 1992 CCCGGG CCC^GGG SphI 1556 GCATGC GCATG^C SspI 4278 AATATT AAT^ATT XbaI 3018 TCTAGA T^CTAGA XmaCI 1992 CCCGGG C^CCGGG XmaI 1992 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### Acc65I 3011 GGTACC G^GTACC 3703 GGTACC AflII 697 CTTAAG C^TTAAG 3785 CTTAAG Alw44I 1647 GTGCAC G^TGCAC 3441 GTGCAC ApaLI 1647 GTGCAC G^TGCAC 3441 GTGCAC Asp718I 3011 GGTACC G^GTACC 3703 GGTACC BbeI 2306 GGCGCC GGCGC^C 2898 GGCGCC BfrI 697 CTTAAG C^TTAAG 3785 CTTAAG BglI 604 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1172 GCCACGTAGGC BglII 872 AGATCT A^GATCT 3214 AGATCT BsaAI 1174 CACGTA [CT]AC^GT[GA] 1369 CACGTG BsaI 4356 GAGACC GGTCTC(1/5) 3510 GGTCTC BsmBI 568 CGTCTC CGTCTC(1/5) 3479 GAGACG Bsp1407I 1688 TGTACA T^GTACA 4250 TGTACA BsrDI 654 CATTGC GCAATG(2/0) 4067 CATTGC BsrGI 1688 TGTACA T^GTACA 4250 TGTACA BssSI 979 CACGAG CACGAG(-5/-1) 2947 CTCGTG BstEII 1056 GGTCACC G^GT[ACGT]ACC 2908 GGTCACC BstPI 1056 GGTCACC G^GT[ACGT]ACC 2908 GGTCACC BstXI 3554 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3794 CCAAAGGCCTGG EcoO65I 1056 GGTCACC G^GT[ACGT]ACC 2908 GGTCACC KasI 2306 GGCGCC G^GCGCC 2898 GGCGCC KpnI 3011 GGTACC GGTAC^C 3703 GGTACC NarI 2306 GGCGCC GG^CGCC 2898 GGCGCC NcoI 227 CCATGG C^CATGG 3930 CCATGG SexAI 989 ACCTGGT A^CC[AT]GGT 2912 ACCTGGT SfoI 2306 GGCGCC GGC^GCC 2898 GGCGCC SspBI 1688 TGTACA T^GTACA 4250 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 132 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApaI ApoI Asp700I AspHI AspI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcgI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII XmnI