##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
Acc65I AflII AgeI AhdI Alw44I
ApaLI AscI Asp718I AspEI AvrII
BciVI BfrI BlnI BsiWI BsmI
BspDI BspLU11I BssSI Bst1107I BstZ17I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco52I EcoNI
EcoT22I FseI HpaI KpnI MluI
NdeI NotI NruI NsiI PacI
PaeR7I PinAI PmeI PshAI PvuI
RsrII SbfI ScaI SgrAI SnaBI
SpeI Sse8387I SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 1573 GACGTC GACGT^C
AccIII 938 TCCGGA T^CCGGA
AclI 4813 AACGTT AA^CGTT
AocI 3898 CCTCAGG CC^T[ACGT]AGG
Aor51HI 4699 AGCGCT AGC^GCT
ApaI 4270 GGGCCC GGGCC^C
AspI 4068 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 941 GGATCC G^GATCC
BbsI 3690 GTCTTC GAAGAC(2/6)
BbvCI 237 GCTGAGG CCTCAGC(-5/-2)
BcgI 1950 CGACGGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 4169 GCCAGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 40 AGATCT A^GATCT
BpuAI 3690 GTCTTC GAAGAC(2/6)
BsaBI 3605 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 938 TCCGGA T^CCGGA
BsiEI 1902 CGACCG CG[GA][CT]^CG
BspEI 938 TCCGGA T^CCGGA
BspHI 3490 TCATGA T^CATGA
BstBI 1825 TTCGAA TT^CGAA
Bsu36I 3898 CCTCAGG CC^T[ACGT]AGG
Eco47III 4699 AGCGCT AGC^GCT
Eco81I 3898 CCTCAGG CC^T[ACGT]AGG
EcoRI 1125 GAATTC G^AATTC
EcoRV 2263 GATATC GAT^ATC
HindIII 3850 AAGCTT A^AGCTT
MamI 3605 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2557 CAATTG C^AATTG
MroI 938 TCCGGA T^CCGGA
MunI 2557 CAATTG C^AATTG
NheI 2074 GCTAGC G^CTAGC
NspV 1825 TTCGAA TT^CGAA
PflFI 4068 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 4813 AACGTT AA^CGTT
RcaI 3490 TCATGA T^CATGA
SalI 2631 GTCGAC G^TCGAC
SfiI 4168 GGCCAGCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1825 TTCGAA TT^CGAA
SmaI 2083 CCCGGG CCC^GGG
SphI 3728 GCATGC GCATG^C
Tth111I 4068 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2083 CCCGGG C^CCGGG
XmaI 2083 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 989 ACGTGT A^C[GA][CT]GT
1754 ACGTGT
AlwNI 1049 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
2161 CAGCGCCTG
Asp700I 133 GAATCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1303 GAAATGATTC
BbeI 2 GGCGCC GGCGC^C
265 GGCGCC
BclI 1346 TGATCA T^GATCA
3604 TGATCA
BsaWI 938 TCCGGA [AT]^CCGG[AT]
1590 TCCGGT
Bsp1407I 992 TGTACA T^GTACA
1234 TGTACA
BsrDI 2927 GCAATG GCAATG(2/0)
2984 GCAATG
BsrGI 992 TGTACA T^GTACA
1234 TGTACA
BstEII 948 GGTAACC G^GT[ACGT]ACC
2004 GGTGACC
BstPI 948 GGTAACC G^GT[ACGT]ACC
2004 GGTGACC
EcoO65I 948 GGTAACC G^GT[ACGT]ACC
2004 GGTGACC
FbaI 1346 TGATCA T^GATCA
3604 TGATCA
HincII 1576 GTCAAC GT[CT]^[GA]AC
2631 GTCGAC
HindII 1576 GTCAAC GT[CT]^[GA]AC
2631 GTCGAC
KasI 2 GGCGCC G^GCGCC
265 GGCGCC
KspI 1921 CCGCGG CCGC^GG
4621 CCGCGG
NaeI 100 GCCGGC GCC^GGC
2836 GCCGGC
NarI 2 GGCGCC GG^CGCC
265 GGCGCC
NgoMIV 100 GCCGGC G^CCGGC
2836 GCCGGC
NspI 3728 GCATGC [GA]CATG^[CT]
3775 GCATGT
SacI 934 GAGCTC GAGCT^C
2814 GAGCTC
SacII 1921 CCGCGG CCGC^GG
4621 CCGCGG
SfoI 2 GGCGCC GGC^GCC
265 GGCGCC
SmlI 3586 CTCAAG C^T[CT][GA]AG
4079 CTTGAG
SspBI 992 TGTACA T^GTACA
1234 TGTACA
StuI 3098 AGGCCT AGG^CCT
3873 AGGCCT
XmnI 133 GAATCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1303 GAAATGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI ApoI AseI
AsnI AspHI AvaI AvaII AviII
BalI BanI BanII BbrPI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssHII
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PflMI PleI
PmaCI PmlI PpuMI PshBI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII