################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## AatII Acc65I AflIII AocI Aor51HI AscI AseI AsnI Asp718I AspI BbeI BbvCI BcgI BclI BlpI Bpu1102I BsmBI BsmI BspDI BspLU11I BstBI Bsu36I CelII ClaI CpoI DraI DrdI EagI EclXI Eco47III Eco52I Eco81I EcoRV EcoT22I FbaI FseI HpaI KasI KpnI MfeI MluI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PmeI PshBI PvuI RsrII SacI SalI SapI SbfI ScaI SexAI SfoI SfuI SgrAI SnaBI SpeI Sse8387I StuI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### AclI 3662 AACGTT AA^CGTT AflII 4141 CTTAAG C^TTAAG AgeI 1913 ACCGGT A^CCGGT Asp700I 2655 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 1370 TGCGCA TGC^GCA BciVI 4333 GTATCC GTATCC(6/5) BfrI 4141 CTTAAG C^TTAAG BglII 2357 AGATCT A^GATCT BsaBI 792 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 3341 GAGACC GGTCTC(1/5) BsiEI 126 CGACCG CG[GA][CT]^CG BsiWI 1617 CGTACG C^GTACG BspHI 2639 TCATGA T^CATGA BssHII 1189 GCGCGC G^CGCGC Bst1107I 3559 GTATAC GTA^TAC BstZ17I 3559 GTATAC GTA^TAC FspI 1370 TGCGCA TGC^GCA HincII 2929 GTCAAC GT[CT]^[GA]AC HindII 2929 GTCAAC GT[CT]^[GA]AC KspI 784 CCGCGG CCGC^GG MamI 792 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC PinAI 1913 ACCGGT A^CCGGT PshAI 3354 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 3662 AACGTT AA^CGTT RcaI 2639 TCATGA T^CATGA SacII 784 CCGCGG CCGC^GG SphI 1367 GCATGC GCATG^C XmnI 2655 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccIII 800 TCCGGA T^CCGGA 2582 TCCGGA AcyI 910 GGCGTC G[GA]^CG[CT]C 3519 GGCGTC AhdI 2488 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3536 GACACATGGTC AspEI 2488 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3536 GACACATGGTC BamHI 582 GGATCC G^GATCC 4186 GGATCC BbrPI 2296 CACGTG CAC^GTG 4221 CACGTG BsaHI 910 GGCGTC G[GA]^CG[CT]C 3519 GGCGTC BseAI 800 TCCGGA T^CCGGA 2582 TCCGGA BspEI 800 TCCGGA T^CCGGA 2582 TCCGGA BsrDI 2324 GCAATG GCAATG(2/0) 2804 GCAATG BssSI 973 CACGAG CACGAG(-5/-1) 1644 CTCGTG BstEII 1936 GGTCACC G^GT[ACGT]ACC 2094 GGTGACC BstPI 1936 GGTCACC G^GT[ACGT]ACC 2094 GGTGACC BstXI 972 CCACGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1037 CCATTTATGTGG Eam1105I 2488 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3536 GACACATGGTC EcoO65I 1936 GGTCACC G^GT[ACGT]ACC 2094 GGTGACC EcoRI 2155 GAATTC G^AATTC 3988 GAATTC HaeII 1329 GGCGCT [GA]GCGC^[CT] 3145 GGCGCT Hin1I 910 GGCGTC G[GA]^CG[CT]C 3519 GGCGTC HindIII 540 AAGCTT A^AGCTT 3293 AAGCTT MroI 800 TCCGGA T^CCGGA 2582 TCCGGA NcoI 2897 CCATGG C^CATGG 3113 CCATGG NspI 1367 GCATGC [GA]CATG^[CT] 1745 GCATGT PmaCI 2296 CACGTG CAC^GTG 4221 CACGTG PmlI 2296 CACGTG CAC^GTG 4221 CACGTG SfiI 2445 GGCCCCAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 2952 GGCCTATGGGGCC SspI 3652 AATATT AAT^ATT 3788 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 130 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI Alw44I AlwI AlwNI ApaI ApaLI ApoI AspHI AvaI AvaII AvrII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BlnI BmyI BpmI BpuAI BsaAI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrBI BsrFI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI