##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AflIII AocI Aor51HI
AscI AseI AsnI Asp718I AspI
BbeI BbvCI BcgI BclI BlpI
Bpu1102I BsmBI BsmI BspDI BspLU11I
BstBI Bsu36I CelII ClaI CpoI
DraI DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoRV EcoT22I FbaI
FseI HpaI KasI KpnI MfeI
MluI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PmeI PshBI PvuI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I StuI SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AclI 3662 AACGTT AA^CGTT
AflII 4141 CTTAAG C^TTAAG
AgeI 1913 ACCGGT A^CCGGT
Asp700I 2655 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1370 TGCGCA TGC^GCA
BciVI 4333 GTATCC GTATCC(6/5)
BfrI 4141 CTTAAG C^TTAAG
BglII 2357 AGATCT A^GATCT
BsaBI 792 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3341 GAGACC GGTCTC(1/5)
BsiEI 126 CGACCG CG[GA][CT]^CG
BsiWI 1617 CGTACG C^GTACG
BspHI 2639 TCATGA T^CATGA
BssHII 1189 GCGCGC G^CGCGC
Bst1107I 3559 GTATAC GTA^TAC
BstZ17I 3559 GTATAC GTA^TAC
FspI 1370 TGCGCA TGC^GCA
HincII 2929 GTCAAC GT[CT]^[GA]AC
HindII 2929 GTCAAC GT[CT]^[GA]AC
KspI 784 CCGCGG CCGC^GG
MamI 792 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PinAI 1913 ACCGGT A^CCGGT
PshAI 3354 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3662 AACGTT AA^CGTT
RcaI 2639 TCATGA T^CATGA
SacII 784 CCGCGG CCGC^GG
SphI 1367 GCATGC GCATG^C
XmnI 2655 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccIII 800 TCCGGA T^CCGGA
2582 TCCGGA
AcyI 910 GGCGTC G[GA]^CG[CT]C
3519 GGCGTC
AhdI 2488 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3536 GACACATGGTC
AspEI 2488 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3536 GACACATGGTC
BamHI 582 GGATCC G^GATCC
4186 GGATCC
BbrPI 2296 CACGTG CAC^GTG
4221 CACGTG
BsaHI 910 GGCGTC G[GA]^CG[CT]C
3519 GGCGTC
BseAI 800 TCCGGA T^CCGGA
2582 TCCGGA
BspEI 800 TCCGGA T^CCGGA
2582 TCCGGA
BsrDI 2324 GCAATG GCAATG(2/0)
2804 GCAATG
BssSI 973 CACGAG CACGAG(-5/-1)
1644 CTCGTG
BstEII 1936 GGTCACC G^GT[ACGT]ACC
2094 GGTGACC
BstPI 1936 GGTCACC G^GT[ACGT]ACC
2094 GGTGACC
BstXI 972 CCACGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1037 CCATTTATGTGG
Eam1105I 2488 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3536 GACACATGGTC
EcoO65I 1936 GGTCACC G^GT[ACGT]ACC
2094 GGTGACC
EcoRI 2155 GAATTC G^AATTC
3988 GAATTC
HaeII 1329 GGCGCT [GA]GCGC^[CT]
3145 GGCGCT
Hin1I 910 GGCGTC G[GA]^CG[CT]C
3519 GGCGTC
HindIII 540 AAGCTT A^AGCTT
3293 AAGCTT
MroI 800 TCCGGA T^CCGGA
2582 TCCGGA
NcoI 2897 CCATGG C^CATGG
3113 CCATGG
NspI 1367 GCATGC [GA]CATG^[CT]
1745 GCATGT
PmaCI 2296 CACGTG CAC^GTG
4221 CACGTG
PmlI 2296 CACGTG CAC^GTG
4221 CACGTG
SfiI 2445 GGCCCCAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2952 GGCCTATGGGGCC
SspI 3652 AATATT AAT^ATT
3788 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI Alw44I AlwI AlwNI ApaI
ApaLI ApoI AspHI AvaI AvaII
AvrII BalI BanI BanII BbsI
BbvI BcnI BfaI BglI BlnI
BmyI BpmI BpuAI BsaAI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspMI BsrBI BsrFI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI