################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## AatII AccI AclI AcsI ApoI AscI AseI AsnI Asp700I BciVI BglII BsiWI Bsp1407I BspDI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI EcoO65I EcoRI EcoRV EcoT22I FseI HincII HindII HindIII HpaI KspI MfeI MunI NaeI NdeI NgoMIV NotI NruI NsiI NspI NspV PacI PaeR7I PmeI PshBI Psp1406I PvuI RsrII SacII SalI ScaI SfiI SfuI SnaBI SpeI SphI SspBI SspI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AflII 4306 CTTAAG C^TTAAG AocI 3616 CCTCAGG CC^T[ACGT]AGG Aor51HI 2872 AGCGCT AGC^GCT AspI 1489 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 2812 TGCGCA TGC^GCA AvrII 4267 CCTAGG C^CTAGG BclI 1069 TGATCA T^GATCA BfrI 4306 CTTAAG C^TTAAG BlnI 4267 CCTAGG C^CTAGG BsiEI 3587 CGGCCG CG[GA][CT]^CG BspHI 2365 TCATGA T^CATGA BsrDI 1959 GCAATG GCAATG(2/0) Bsu36I 3616 CCTCAGG CC^T[ACGT]AGG EagI 3587 CGGCCG C^GGCCG EclXI 3587 CGGCCG C^GGCCG Eco47III 2872 AGCGCT AGC^GCT Eco52I 3587 CGGCCG C^GGCCG Eco81I 3616 CCTCAGG CC^T[ACGT]AGG FbaI 1069 TGATCA T^GATCA FspI 2812 TGCGCA TGC^GCA MluI 2284 ACGCGT A^CGCGT NcoI 3448 CCATGG C^CATGG NheI 417 GCTAGC G^CTAGC PflFI 1489 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC RcaI 2365 TCATGA T^CATGA SbfI 1817 CCTGCAGG CCTGCA^GG SexAI 191 ACCTGGT A^CC[AT]GGT SgrAI 2680 CACCGGTG C[GA]^CCGG[CT]G Sse8387I 1817 CCTGCAGG CCTGCA^GG TfiI 790 GATTC G^A[AT]TC Tth111I 1489 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### Acc65I 1907 GGTACC G^GTACC 2800 GGTACC AflIII 2284 ACGCGT A^C[GA][CT]GT 2785 ACGTGT AgeI 2681 ACCGGT A^CCGGT 3345 ACCGGT AhdI 468 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1845 GACTGAGAGTC Alw44I 699 GTGCAC G^TGCAC 3813 GTGCAC ApaI 1790 GGGCCC GGGCC^C 2643 GGGCCC ApaLI 699 GTGCAC G^TGCAC 3813 GTGCAC Asp718I 1907 GGTACC G^GTACC 2800 GGTACC AspEI 468 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1845 GACTGAGAGTC BamHI 1206 GGATCC G^GATCC 1390 GGATCC BbrPI 2268 CACGTG CAC^GTG 2805 CACGTG BcgI 3712 GCACACCATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3919 GCATGGCCTTCG BlpI 986 GCTGAGC GC^T[ACGT]AGC 2152 GCTGAGC Bpu1102I 986 GCTGAGC GC^T[ACGT]AGC 2152 GCTGAGC BsaAI 2268 CACGTG [CT]AC^GT[GA] 2805 CACGTG BsaBI 3773 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3779 GATCAAGATC BsmBI 1487 GAGACG CGTCTC(1/5) 4374 GAGACG BsmI 498 GAATGC GAATGC(1/-1) 3962 GCATTC BsrBI 3305 CCGCTC CCGCTC(-3/-3) 3417 GAGCGG CelII 986 GCTGAGC GC^T[ACGT]AGC 2152 GCTGAGC DrdI 1676 GACAGGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2065 GACTCCTCTGTC Eam1105I 468 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1845 GACTGAGAGTC KpnI 1907 GGTACC GGTAC^C 2800 GGTACC MamI 3773 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3779 GATCAAGATC MslI 1744 CACAGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2678 CACACCGGTG PinAI 2681 ACCGGT A^CCGGT 3345 ACCGGT PmaCI 2268 CACGTG CAC^GTG 2805 CACGTG PmlI 2268 CACGTG CAC^GTG 2805 CACGTG PshAI 2990 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3975 GACTACAGTC SapI 2526 GAAGAGC GCTCTTC(1/4) 3131 GAAGAGC XcmI 2402 CCACCGCACTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4330 CCACCACCCTGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 129 enzymes) # ######################################### AccII AccIII AciI AcyI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BalI BanI BanII BbeI BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspEI BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MroI MscI MseI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI StuI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmaCI XmaI