##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AccI AclI AcsI ApoI
AscI AseI AsnI Asp700I BciVI
BglII BsiWI Bsp1407I BspDI BspLU11I
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI EcoO65I EcoRI EcoRV EcoT22I
FseI HincII HindII HindIII HpaI
KspI MfeI MunI NaeI NdeI
NgoMIV NotI NruI NsiI NspI
NspV PacI PaeR7I PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
ScaI SfiI SfuI SnaBI SpeI
SphI SspBI SspI SwaI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AflII 4306 CTTAAG C^TTAAG
AocI 3616 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2872 AGCGCT AGC^GCT
AspI 1489 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2812 TGCGCA TGC^GCA
AvrII 4267 CCTAGG C^CTAGG
BclI 1069 TGATCA T^GATCA
BfrI 4306 CTTAAG C^TTAAG
BlnI 4267 CCTAGG C^CTAGG
BsiEI 3587 CGGCCG CG[GA][CT]^CG
BspHI 2365 TCATGA T^CATGA
BsrDI 1959 GCAATG GCAATG(2/0)
Bsu36I 3616 CCTCAGG CC^T[ACGT]AGG
EagI 3587 CGGCCG C^GGCCG
EclXI 3587 CGGCCG C^GGCCG
Eco47III 2872 AGCGCT AGC^GCT
Eco52I 3587 CGGCCG C^GGCCG
Eco81I 3616 CCTCAGG CC^T[ACGT]AGG
FbaI 1069 TGATCA T^GATCA
FspI 2812 TGCGCA TGC^GCA
MluI 2284 ACGCGT A^CGCGT
NcoI 3448 CCATGG C^CATGG
NheI 417 GCTAGC G^CTAGC
PflFI 1489 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 2365 TCATGA T^CATGA
SbfI 1817 CCTGCAGG CCTGCA^GG
SexAI 191 ACCTGGT A^CC[AT]GGT
SgrAI 2680 CACCGGTG C[GA]^CCGG[CT]G
Sse8387I 1817 CCTGCAGG CCTGCA^GG
TfiI 790 GATTC G^A[AT]TC
Tth111I 1489 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 1907 GGTACC G^GTACC
2800 GGTACC
AflIII 2284 ACGCGT A^C[GA][CT]GT
2785 ACGTGT
AgeI 2681 ACCGGT A^CCGGT
3345 ACCGGT
AhdI 468 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1845 GACTGAGAGTC
Alw44I 699 GTGCAC G^TGCAC
3813 GTGCAC
ApaI 1790 GGGCCC GGGCC^C
2643 GGGCCC
ApaLI 699 GTGCAC G^TGCAC
3813 GTGCAC
Asp718I 1907 GGTACC G^GTACC
2800 GGTACC
AspEI 468 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1845 GACTGAGAGTC
BamHI 1206 GGATCC G^GATCC
1390 GGATCC
BbrPI 2268 CACGTG CAC^GTG
2805 CACGTG
BcgI 3712 GCACACCATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3919 GCATGGCCTTCG
BlpI 986 GCTGAGC GC^T[ACGT]AGC
2152 GCTGAGC
Bpu1102I 986 GCTGAGC GC^T[ACGT]AGC
2152 GCTGAGC
BsaAI 2268 CACGTG [CT]AC^GT[GA]
2805 CACGTG
BsaBI 3773 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3779 GATCAAGATC
BsmBI 1487 GAGACG CGTCTC(1/5)
4374 GAGACG
BsmI 498 GAATGC GAATGC(1/-1)
3962 GCATTC
BsrBI 3305 CCGCTC CCGCTC(-3/-3)
3417 GAGCGG
CelII 986 GCTGAGC GC^T[ACGT]AGC
2152 GCTGAGC
DrdI 1676 GACAGGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2065 GACTCCTCTGTC
Eam1105I 468 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1845 GACTGAGAGTC
KpnI 1907 GGTACC GGTAC^C
2800 GGTACC
MamI 3773 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3779 GATCAAGATC
MslI 1744 CACAGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2678 CACACCGGTG
PinAI 2681 ACCGGT A^CCGGT
3345 ACCGGT
PmaCI 2268 CACGTG CAC^GTG
2805 CACGTG
PmlI 2268 CACGTG CAC^GTG
2805 CACGTG
PshAI 2990 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3975 GACTACAGTC
SapI 2526 GAAGAGC GCTCTTC(1/4)
3131 GAAGAGC
XcmI 2402 CCACCGCACTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4330 CCACCACCCTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AluI AlwI AlwNI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbsI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspEI BspMI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MroI MscI
MseI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI StuI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmaCI XmaI