##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII AccII AclI AflII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI AspEI AviII
AvrII BalI BbrPI BciVI BclI
BfrI BlnI BsaBI BsiWI BspDI
BsrFI BssHII Bst1107I BstAPI BstBI
BstUI BstZ17I Cfr10I ClaI CpoI
DrdI Eam1105I Eco47III EcoRI EcoT22I
FbaI FseI FspI KspI MamI
MfeI MluI MluNI MscI MunI
MvnI NaeI NcoI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacII SalI ScaI SfiI SfuI
SgrAI SmaI SpeI SphI SwaI
Van91I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 2871 GGTACC G^GTACC
AccI 2861 GTCTAC GT^[AC][GT]AC
AflIII 980 ACATGT A^C[GA][CT]GT
Asp700I 209 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2871 GGTACC G^GTACC
AspI 1702 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 814 GAAGAC GAAGAC(2/6)
BglI 3361 GCCAGCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2517 AGATCT A^GATCT
BpuAI 814 GAAGAC GAAGAC(2/6)
BsaAI 340 TACGTA [CT]AC^GT[GA]
Bsp1407I 2145 TGTACA T^GTACA
BspHI 1194 TCATGA T^CATGA
BspLU11I 980 ACATGT A^CATGT
BspMI 1624 ACCTGC ACCTGC(4/8)
BsrGI 2145 TGTACA T^GTACA
BssSI 2837 CACGAG CACGAG(-5/-1)
BstEII 729 GGTCACC G^GT[ACGT]ACC
BstPI 729 GGTCACC G^GT[ACGT]ACC
DsaI 1464 CCACGG C^C[GA][CT]GG
EcoO65I 729 GGTCACC G^GT[ACGT]ACC
EcoRV 1358 GATATC GAT^ATC
EcoT14I 3402 CCAAGG C^C[AT][AT]GG
HindIII 380 AAGCTT A^AGCTT
HpaI 1696 GTTAAC GTT^AAC
KpnI 2871 GGTACC GGTAC^C
NheI 475 GCTAGC G^CTAGC
PflFI 1702 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1194 TCATGA T^CATGA
SacI 3338 GAGCTC GAGCT^C
SbfI 1625 CCTGCAGG CCTGCA^GG
SnaBI 340 TACGTA TAC^GTA
Sse8387I 1625 CCTGCAGG CCTGCA^GG
SspBI 2145 TGTACA T^GTACA
StuI 3378 AGGCCT AGG^CCT
StyI 3402 CCAAGG C^C[AT][AT]GG
Tth111I 1702 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 209 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccIII 420 TCCGGA T^CCGGA
3481 TCCGGA
AocI 3079 CCTGAGG CC^T[ACGT]AGG
3281 CCTCAGG
BamHI 2250 GGATCC G^GATCC
3937 GGATCC
BbeI 1577 GGCGCC GGCGC^C
3432 GGCGCC
BcgI 2906 CGAGAAATATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
357 GCACCGTGTTCG
BseAI 420 TCCGGA T^CCGGA
3481 TCCGGA
BsiEI 31 CGGCCG CG[GA][CT]^CG
992 CGGCCG
BspEI 420 TCCGGA T^CCGGA
3481 TCCGGA
BsrBI 958 CCGCTC CCGCTC(-3/-3)
1751 GAGCGG
BsrDI 2381 CATTGC GCAATG(2/0)
3276 CATTGC
BstXI 1299 CCAGGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3236 CCAGCTCCCTGG
Bsu36I 3079 CCTGAGG CC^T[ACGT]AGG
3281 CCTCAGG
DraI 3656 TTTAAA TTT^AAA
3735 TTTAAA
EagI 31 CGGCCG C^GGCCG
992 CGGCCG
EclXI 31 CGGCCG C^GGCCG
992 CGGCCG
Eco52I 31 CGGCCG C^GGCCG
992 CGGCCG
Eco81I 3079 CCTGAGG CC^T[ACGT]AGG
3281 CCTCAGG
EcoNI 184 CCTCCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1135 CCTGAGCGAGG
KasI 1577 GGCGCC G^GCGCC
3432 GGCGCC
MroI 420 TCCGGA T^CCGGA
3481 TCCGGA
MslI 1996 CATGCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2149 CATTGAGGTG
NarI 1577 GGCGCC GG^CGCC
3432 GGCGCC
PpuMI 585 GGGACCC [GA]G^G[AT]CC[CT]
4300 GGGACCT
SapI 1150 GAAGAGC GCTCTTC(1/4)
2746 GCTCTTC
SfoI 1577 GGCGCC GGC^GCC
3432 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BanI BanII BbvCI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DraIII EaeI
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PshAI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII