##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AhdI Aor51HI AscI Asp718I
AspEI AspI AvaI AviII AvrII
BbeI BbrPI BcgI BciVI BlnI
BlpI Bpu1102I BsaAI BsaHI BseAI
BsiWI BsoBI BspDI BspEI BspHI
BsrBI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
CelII ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV FauI FseI FspI
HaeII HgaI Hin1I KasI KpnI
KspI MfeI MluI MroI MunI
MvnI NaeI NarI NgoMIV NotI
NruI NspV PaeR7I PflFI PmaCI
PmlI PpuMI PshAI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SwaI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 223 GTCGAC GT^[AC][GT]AC
AgeI 219 ACCGGT A^CCGGT
AocI 3355 CCTAAGG CC^T[ACGT]AGG
ApaI 394 GGGCCC GGGCC^C
Asp700I 1331 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 181 GGATCC G^GATCC
BanII 394 GGGCCC G[GA]GC[CT]^C
BbvCI 171 CCTCAGC CCTCAGC(-5/-2)
BclI 2163 TGATCA T^GATCA
BcnI 603 CCGGG CC^[GC]GG
BglI 3988 GCCTTTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 348 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3252 GAGACC GGTCTC(1/5)
BsaWI 219 ACCGGT [AT]^CCGG[AT]
BsiEI 221 CGGTCG CG[GA][CT]^CG
BsmBI 550 GAGACG CGTCTC(1/5)
Bsp1407I 3429 TGTACA T^GTACA
BsrFI 219 ACCGGT [GA]^CCGG[CT]
BsrGI 3429 TGTACA T^GTACA
BssSI 1743 CTCGTG CACGAG(-5/-1)
Bsu36I 3355 CCTAAGG CC^T[ACGT]AGG
CfoI 2417 GCGC GCG^C
Cfr10I 219 ACCGGT [GA]^CCGG[CT]
Eco81I 3355 CCTAAGG CC^T[ACGT]AGG
EcoNI 2384 CCTTTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 2163 TGATCA T^GATCA
HhaI 2417 GCGC GCG^C
HinP1I 2417 GCGC G^CGC
HphI 1842 GGTGA GGTGA(8/7)
MamI 348 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NciI 603 CCGGG CC^[GC]GG
PinAI 219 ACCGGT A^CCGGT
PmeI 3856 GTTTAAAC GTTT^AAAC
PstI 314 CTGCAG CTGCA^G
SalI 223 GTCGAC G^TCGAC
SapI 4447 GAAGAGC GCTCTTC(1/4)
ScaI 4231 AGTACT AGT^ACT
SspBI 3429 TGTACA T^GTACA
StuI 3927 AGGCCT AGG^CCT
XbaI 1698 TCTAGA T^CTAGA
XcmI 288 CCATATCAGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1331 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflII 885 CTTAAG C^TTAAG
3577 CTTAAG
Alw44I 336 GTGCAC G^TGCAC
1484 GTGCAC
ApaLI 336 GTGCAC G^TGCAC
1484 GTGCAC
BfrI 885 CTTAAG C^TTAAG
3577 CTTAAG
BglII 1445 AGATCT A^GATCT
3791 AGATCT
BsgI 274 GTGCAG GTGCAG(16/14)
757 GTGCAG
BsmI 2735 GCATTC GAATGC(1/-1)
2995 GCATTC
BspMI 759 GCAGGT ACCTGC(4/8)
2362 GCAGGT
BsrDI 261 CATTGC GCAATG(2/0)
4387 GCAATG
Cac8I 2796 GCTAGC GC[ACGT]^[ACGT]GC
3694 GCTAGC
DsaI 269 CCATGG C^C[GA][CT]GG
329 CCATGG
EcoRI 457 GAATTC G^AATTC
846 GAATTC
EcoT22I 1153 ATGCAT ATGCA^T
1801 ATGCAT
HapII 220 CCGG C^CGG
603 CCGG
HpaI 2060 GTTAAC GTT^AAC
2611 GTTAAC
HpaII 220 CCGG C^CGG
603 CCGG
MspA1I 137 CAGCGG C[AC]G^C[GT]G
294 CAGCGG
MspI 220 CCGG C^CGG
603 CCGG
NcoI 269 CCATGG C^CATGG
329 CCATGG
NheI 2796 GCTAGC G^CTAGC
3694 GCTAGC
NsiI 1153 ATGCAT ATGCA^T
1801 ATGCAT
PacI 2841 TTAATTAA TTAAT^TAA
4513 TTAATTAA
PflMI 188 CCATCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
384 CCAGTGCCTGG
Van91I 188 CCATCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
384 CCAGTGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AseI AsnI
AspHI AvaII BalI BanI BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspLU11I BsrI
BssKI BstF5I BstNI BstYI Cfr13I
DdeI DpnII DraI DraII EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HincII HindII HindIII
HinfI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MvaI
MwoI NdeI NdeII NlaIII NlaIV
NspI PleI PshBI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII