##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
Acc65I AclI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspI BamHI
BglI BglII BlpI Bpu1102I BsiWI
BspDI BsrDI BssHII Bst1107I BstEII
BstPI BstZ17I CelII ClaI CpoI
DraIII EagI EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FseI
HpaI KpnI KspI NaeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PmeI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SfiI SnaBI SpeI Sse8387I SwaI
Tth111I XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 1054 GACGTC GACGT^C
AccI 3368 GTCTAC GT^[AC][GT]AC
AccIII 519 TCCGGA T^CCGGA
AgeI 1919 ACCGGT A^CCGGT
Alw44I 1940 GTGCAC G^TGCAC
ApaLI 1940 GTGCAC G^TGCAC
AviII 1042 TGCGCA TGC^GCA
BbeI 728 GGCGCC GGCGC^C
BbrPI 1046 CACGTG CAC^GTG
BbsI 1286 GTCTTC GAAGAC(2/6)
BciVI 4206 GTATCC GTATCC(6/5)
BpuAI 1286 GTCTTC GAAGAC(2/6)
BsaAI 1046 CACGTG [CT]AC^GT[GA]
BsaBI 747 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 519 TCCGGA T^CCGGA
BsiEI 3250 CGACCG CG[GA][CT]^CG
BsmBI 1016 CGTCTC CGTCTC(1/5)
Bsp1407I 2624 TGTACA T^GTACA
BspEI 519 TCCGGA T^CCGGA
BspHI 3434 TCATGA T^CATGA
BspLU11I 3648 ACATGT A^CATGT
BsrFI 1919 ACCGGT [GA]^CCGG[CT]
BsrGI 2624 TGTACA T^GTACA
BssSI 2146 CTCGTG CACGAG(-5/-1)
BstBI 3032 TTCGAA TT^CGAA
Cfr10I 1919 ACCGGT [GA]^CCGG[CT]
DraI 1757 TTTAAA TTT^AAA
DrdI 837 GACCATCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 1042 TGCGCA TGC^GCA
HgaI 683 GACGC GACGC(5/10)
HincII 3318 GTTGAC GT[CT]^[GA]AC
HindII 3318 GTTGAC GT[CT]^[GA]AC
HindIII 3393 AAGCTT A^AGCTT
KasI 728 GGCGCC G^GCGCC
MamI 747 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 489 CAATTG C^AATTG
MluI 954 ACGCGT A^CGCGT
MroI 519 TCCGGA T^CCGGA
MunI 489 CAATTG C^AATTG
NarI 728 GGCGCC GG^CGCC
NdeI 230 CATATG CA^TATG
NspV 3032 TTCGAA TT^CGAA
PinAI 1919 ACCGGT A^CCGGT
PmaCI 1046 CACGTG CAC^GTG
PmlI 1046 CACGTG CAC^GTG
PshAI 3288 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3434 TCATGA T^CATGA
ScaI 671 AGTACT AGT^ACT
SexAI 533 ACCTGGT A^CC[AT]GGT
SfoI 728 GGCGCC GGC^GCC
SfuI 3032 TTCGAA TT^CGAA
SgrAI 1918 CACCGGTG C[GA]^CCGG[CT]G
SphI 3716 GCATGC GCATG^C
SspBI 2624 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 955 CGCG CG^CG
1305 CGCG
AcsI 3876 AAATTT [GA]^AATT[CT]
4079 AAATTC
AcyI 728 GGCGCC G[GA]^CG[CT]C
1054 GACGTC
AflII 1315 CTTAAG C^TTAAG
3635 CTTAAG
AhdI 468 GACTCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2030 GACACCTAGTC
ApoI 3876 AAATTT [GA]^AATT[CT]
4079 AAATTC
AspEI 468 GACTCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2030 GACACCTAGTC
AvrII 1594 CCTAGG C^CTAGG
2105 CCTAGG
BbvCI 592 GCTGAGG CCTCAGC(-5/-2)
3660 GCTGAGG
BcgI 2368 CGATGGGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
833 GCAAGACCATCG
BclI 620 TGATCA T^GATCA
1268 TGATCA
BfrI 1315 CTTAAG C^TTAAG
3635 CTTAAG
BlnI 1594 CCTAGG C^CTAGG
2105 CCTAGG
BsaHI 728 GGCGCC G[GA]^CG[CT]C
1054 GACGTC
BsaWI 519 TCCGGA [AT]^CCGG[AT]
1919 ACCGGT
BsmI 2495 GAATGC GAATGC(1/-1)
4026 GCATTC
BsrBI 3678 CCGCTC CCGCTC(-3/-3)
1007 GAGCGG
BstUI 955 CGCG CG^CG
1305 CGCG
BstYI 1986 GGATCT [GA]^GATC[CT]
3280 GGATCT
Eam1105I 468 GACTCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2030 GACACCTAGTC
FbaI 620 TGATCA T^GATCA
1268 TGATCA
HaeII 728 GGCGCC [GA]GCGC^[CT]
1194 GGCGCT
Hin1I 728 GGCGCC G[GA]^CG[CT]C
1054 GACGTC
MflI 1986 GGATCT [GA]^GATC[CT]
3280 GGATCT
MvnI 955 CGCG CG^CG
1305 CGCG
PvuII 1958 CAGCTG CAG^CTG
2177 CAGCTG
SacI 562 GAGCTC GAGCT^C
3843 GAGCTC
SspI 3460 AATATT AAT^ATT
4228 AATATT
XbaI 2039 TCTAGA T^CTAGA
3883 TCTAGA
XhoII 1986 GGATCT [GA]^GATC[CT]
3280 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BalI BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstXI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XmaCI XmaI