##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AccI AclI AflIII AgeI
AseI AsnI Asp700I BamHI BbrPI
BbsI BciVI BpuAI BsaAI BsaBI
BsiWI BsmBI BsmI BspHI BspLU11I
Bst1107I BstZ17I DraI EcoRV EcoT22I
FseI HincII HindII HindIII HpaI
KspI MamI MfeI MluI MunI
NdeI NheI NotI NsiI PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI SacI
SacII SalI SapI SfiI SgrAI
SmaI SnaBI SspI SwaI XbaI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AcsI 3117 GAATTC [GA]^AATT[CT]
AflII 4343 CTTAAG C^TTAAG
Aor51HI 202 AGCGCT AGC^GCT
ApoI 3117 GAATTC [GA]^AATT[CT]
AscI 136 GGCGCGCC GG^CGCGCC
AviII 1485 TGCGCA TGC^GCA
BclI 2309 TGATCA T^GATCA
BfrI 4343 CTTAAG C^TTAAG
BglII 2156 AGATCT A^GATCT
Bsp1407I 2204 TGTACA T^GTACA
BspDI 3577 ATCGAT AT^CGAT
BsrDI 1595 GCAATG GCAATG(2/0)
BsrGI 2204 TGTACA T^GTACA
BssSI 3910 CACGAG CACGAG(-5/-1)
BstBI 2877 TTCGAA TT^CGAA
BstEII 4140 GGTGACC G^GT[ACGT]ACC
BstPI 4140 GGTGACC G^GT[ACGT]ACC
ClaI 3577 ATCGAT AT^CGAT
CpoI 156 CGGACCG CG^G[AT]CCG
DrdI 3040 GACCCTGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 3629 CCATGG C^C[GA][CT]GG
EagI 379 CGGCCG C^GGCCG
EclXI 379 CGGCCG C^GGCCG
Eco47III 202 AGCGCT AGC^GCT
Eco52I 379 CGGCCG C^GGCCG
EcoO65I 4140 GGTGACC G^GT[ACGT]ACC
EcoRI 3117 GAATTC G^AATTC
FbaI 2309 TGATCA T^GATCA
FspI 1485 TGCGCA TGC^GCA
MaeII 4488 ACGT A^CGT
NcoI 3629 CCATGG C^CATGG
NruI 2145 TCGCGA TCG^CGA
NspV 2877 TTCGAA TT^CGAA
RsrII 156 CGGACCG CG^G[AT]CCG
SbfI 1719 CCTGCAGG CCTGCA^GG
ScaI 1694 AGTACT AGT^ACT
SfuI 2877 TTCGAA TT^CGAA
SpeI 2420 ACTAGT A^CTAGT
Sse8387I 1719 CCTGCAGG CCTGCA^GG
SspBI 2204 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AhdI 3634 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3972 GACCCTGTGTC
Alw44I 3019 GTGCAC G^TGCAC
3489 GTGCAC
ApaLI 3019 GTGCAC G^TGCAC
3489 GTGCAC
AspEI 3634 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3972 GACCCTGTGTC
AspI 2970 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4211 GACAGTGTC
AvrII 664 CCTAGG C^CTAGG
3161 CCTAGG
BalI 1059 TGGCCA TGG^CCA
2593 TGGCCA
BbvCI 3743 CCTCAGC CCTCAGC(-5/-2)
3008 GCTGAGG
BcgI 3618 CGAAGCAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
569 GCAGGAAAGTCG
BlnI 664 CCTAGG C^CTAGG
3161 CCTAGG
BsaI 609 GAGACC GGTCTC(1/5)
2277 GAGACC
BsiEI 152 CGGTCG CG[GA][CT]^CG
379 CGGCCG
BstAPI 404 GCACTTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3021 GCACAGTGTGC
DraIII 888 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
3022 CACAGTGTG
Eam1105I 3634 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3972 GACCCTGTGTC
HgaI 2216 GACGC GACGC(5/10)
2974 GCGTC
MluNI 1059 TGGCCA TGG^CCA
2593 TGGCCA
MscI 1059 TGGCCA TGG^CCA
2593 TGGCCA
MseI 1581 TTAA T^TAA
4344 TTAA
NspI 1591 GCATGC [GA]CATG^[CT]
3202 GCATGC
PaeR7I 1201 CTCGAG C^TCGAG
1371 CTCGAG
PflFI 2970 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4211 GACAGTGTC
SexAI 782 ACCAGGT A^CC[AT]GGT
2452 ACCTGGT
SphI 1591 GCATGC GCATG^C
3202 GCATGC
Tru9I 1581 TTAA T^TAA
4344 TTAA
Tth111I 2970 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4211 GACAGTGTC
XhoI 1201 CTCGAG C^TCGAG
1371 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
Acc65I AccII AccIII AciI AcyI
AfaI AluI AlwI AlwNI AocI
ApaI Asp718I AspHI AvaI AvaII
BanI BanII BbeI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaHI BsaJI BsaWI BseAI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspEI
BspMI BsrBI BsrFI BsrI BssHII
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI KpnI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MroI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI StuI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII