##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
Acc65I AccIII AflII AgeI AhdI
Aor51HI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BbvCI
BfrI BlnI BseAI BsiWI BspEI
BspHI BspLU11I Bst1107I BstBI BstEII
BstPI BstZ17I DraI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I FseI
FspI HindIII HpaI KpnI MluI
MroI NotI NruI NspV PacI
PflFI PinAI PmeI PshAI PshBI
PvuI RcaI SbfI ScaI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AatII 3978 GACGTC GACGT^C
AccI 3222 GTCGAC GT^[AC][GT]AC
AclI 1262 AACGTT AA^CGTT
AocI 4290 CCTGAGG CC^T[ACGT]AGG
Asp700I 2085 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 1434 CACGTG CAC^GTG
BcgI 179 CGAGTCGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 709 GTATCC GTATCC(6/5)
BclI 1159 TGATCA T^GATCA
BglII 121 AGATCT A^GATCT
BsaBI 704 GATGGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 644 ACCGGA [AT]^CCGG[AT]
BsiEI 3175 CGACCG CG[GA][CT]^CG
BspMI 1773 ACCTGC ACCTGC(4/8)
BssSI 3423 CACGAG CACGAG(-5/-1)
Bsu36I 4290 CCTGAGG CC^T[ACGT]AGG
CpoI 1467 CGGTCCG CG^G[AT]CCG
Eco81I 4290 CCTGAGG CC^T[ACGT]AGG
EcoRV 2301 GATATC GAT^ATC
EcoT22I 3229 ATGCAT ATGCA^T
FbaI 1159 TGATCA T^GATCA
MamI 704 GATGGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1165 CAATTG C^AATTG
MunI 1165 CAATTG C^AATTG
NdeI 4353 CATATG CA^TATG
NsiI 3229 ATGCAT ATGCA^T
PmaCI 1434 CACGTG CAC^GTG
PmlI 1434 CACGTG CAC^GTG
Psp1406I 1262 AACGTT AA^CGTT
RsrII 1467 CGGTCCG CG^G[AT]CCG
SalI 3222 GTCGAC G^TCGAC
SexAI 2963 ACCTGGT A^CC[AT]GGT
SphI 432 GCATGC GCATG^C
XmnI 2085 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Alw44I 1488 GTGCAC G^TGCAC
2241 GTGCAC
ApaLI 1488 GTGCAC G^TGCAC
2241 GTGCAC
BamHI 2673 GGATCC G^GATCC
4460 GGATCC
BsaAI 1434 CACGTG [CT]AC^GT[GA]
2385 TACGTG
Bsp1407I 1864 TGTACA T^GTACA
4755 TGTACA
BsrDI 2863 CATTGC GCAATG(2/0)
3232 CATTGC
BsrGI 1864 TGTACA T^GTACA
4755 TGTACA
BssHII 495 GCGCGC G^CGCGC
544 GCGCGC
DraIII 2962 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
3777 CACGGTGTG
DrdI 1313 GACTGAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3213 GACTCTGCTGTC
EcoRI 260 GAATTC G^AATTC
1445 GAATTC
MslI 554 CACGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2381 CATCTACGTG
NaeI 4143 GCCGGC GCC^GGC
4218 GCCGGC
NgoMIV 4143 GCCGGC G^CCGGC
4218 GCCGGC
NheI 858 GCTAGC G^CTAGC
2784 GCTAGC
NspI 432 GCATGC [GA]CATG^[CT]
2509 ACATGC
PaeR7I 50 CTCGAG C^TCGAG
4485 CTCGAG
PstI 1775 CTGCAG CTGCA^G
4258 CTGCAG
PvuII 577 CAGCTG CAG^CTG
876 CAGCTG
SspBI 1864 TGTACA T^GTACA
4755 TGTACA
StuI 3684 AGGCCT AGG^CCT
4413 AGGCCT
XbaI 124 TCTAGA T^CTAGA
3836 TCTAGA
XcmI 2702 CCAGGGCCTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4305 CCAGGCCCGGAATGG
XhoI 50 CTCGAG C^TCGAG
4485 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbeI BbsI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspDI BsrBI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I ClaI DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV PflMI
PleI PpuMI RsaI SacI SacII
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmaCI XmaI