##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AhdI Alw44I AocI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
BciVI BclI BfrI BsiWI BspDI
BspLU11I BsrDI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
ClaI CpoI Eam1105I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HindIII KpnI KspI MfeI MunI
NdeI NheI NotI NruI NsiI
NspV PaeR7I PflFI PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 26 enzymes) #
###################################
AgeI 1867 ACCGGT A^CCGGT
AviII 3793 TGCGCA TGC^GCA
AvrII 856 CCTAGG C^CTAGG
BcgI 1682 CGACGGGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 856 CCTAGG C^CTAGG
BsaBI 2252 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 3494 CGTCTC CGTCTC(1/5)
Bsp1407I 4408 TGTACA T^GTACA
BsrGI 4408 TGTACA T^GTACA
DraI 4870 TTTAAA TTT^AAA
DrdI 2424 GACCCCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1190 CCTACAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 3793 TGCGCA TGC^GCA
HincII 505 GTTAAC GT[CT]^[GA]AC
HindII 505 GTTAAC GT[CT]^[GA]AC
HpaI 505 GTTAAC GTT^AAC
MamI 2252 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 34 ACGCGT A^CGCGT
PacI 711 TTAATTAA TTAAT^TAA
PinAI 1867 ACCGGT A^CCGGT
PshAI 447 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2163 GAGCTC GAGCT^C
SapI 3995 GAAGAGC GCTCTTC(1/4)
SphI 4111 GCATGC GCATG^C
SspBI 4408 TGTACA T^GTACA
StuI 3367 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccII 35 CGCG CG^CG
4238 CGCG
Asp700I 2339 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3572 GAAGATCTTC
BbrPI 705 CACGTG CAC^GTG
2130 CACGTG
BglII 3514 AGATCT A^GATCT
3574 AGATCT
BsaAI 705 CACGTG [CT]AC^GT[GA]
2130 CACGTG
BstUI 35 CGCG CG^CG
4238 CGCG
EagI 1840 CGGCCG C^GGCCG
2756 CGGCCG
EclXI 1840 CGGCCG C^GGCCG
2756 CGGCCG
Eco52I 1840 CGGCCG C^GGCCG
2756 CGGCCG
MvnI 35 CGCG CG^CG
4238 CGCG
NaeI 2962 GCCGGC GCC^GGC
3765 GCCGGC
NgoMIV 2962 GCCGGC G^CCGGC
3765 GCCGGC
NspI 404 ACATGC [GA]CATG^[CT]
4111 GCATGC
PmaCI 705 CACGTG CAC^GTG
2130 CACGTG
PmlI 705 CACGTG CAC^GTG
2130 CACGTG
SmlI 631 CTTGAG C^T[CT][GA]AG
2940 CTCAAG
TaqI 302 TCGA T^CGA
1358 TCGA
TthHB8I 302 TCGA T^CGA
1358 TCGA
XcmI 123 CCAGCGCTGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3868 CCATCCTCATCATGG
XmnI 2339 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3572 GAAGATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccIII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI Aor51HI
ApaI ApoI AspHI AvaI AvaII
BalI BamHI BanI BanII BbeI
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco47III EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MroI
MscI MseI MslI MspA1I MspI
MvaI MwoI NarI NciI NcoI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SfoI SmaI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XhoII XmaCI XmaI