##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AccI AclI AflII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI AspI AviII AvrII BbeI
BbrPI BciVI BclI BfrI BlnI
BsaAI BsaBI BsiWI BspHI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
CpoI DraI Eco47III EcoO65I FbaI
FseI FspI HindIII HpaI KasI
MamI NarI NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SbfI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 1399 GACGCC G[GA]^CG[CT]C
AhdI 2128 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1790 CCTCAGG CC^T[ACGT]AGG
AspEI 2128 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 481 CCTCAGC CCTCAGC(-5/-2)
BsaHI 1399 GACGCC G[GA]^CG[CT]C
Bsp1407I 1766 TGTACA T^GTACA
BspDI 4102 ATCGAT AT^CGAT
BsrGI 1766 TGTACA T^GTACA
BssSI 2928 CTCGTG CACGAG(-5/-1)
BstAPI 563 GCAAGATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1790 CCTCAGG CC^T[ACGT]AGG
ClaI 4102 ATCGAT AT^CGAT
EagI 3914 CGGCCG C^GGCCG
Eam1105I 2128 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3914 CGGCCG C^GGCCG
Eco52I 3914 CGGCCG C^GGCCG
Eco81I 1790 CCTCAGG CC^T[ACGT]AGG
EcoRV 2580 GATATC GAT^ATC
EcoT22I 1071 ATGCAT ATGCA^T
Hin1I 1399 GACGCC G[GA]^CG[CT]C
HincII 4887 GTTGAC GT[CT]^[GA]AC
HindII 4887 GTTGAC GT[CT]^[GA]AC
NsiI 1071 ATGCAT ATGCA^T
SapI 4599 GAAGAGC GCTCTTC(1/4)
ScaI 4625 AGTACT AGT^ACT
SphI 1834 GCATGC GCATG^C
SspBI 1766 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 2840 GGTACC G^GTACC
3310 GGTACC
AcsI 2747 GAATTC [GA]^AATT[CT]
4287 GAATTC
ApoI 2747 GAATTC [GA]^AATT[CT]
4287 GAATTC
Asp718I 2840 GGTACC G^GTACC
3310 GGTACC
BamHI 1702 GGATCC G^GATCC
1953 GGATCC
BfaI 234 CTAG C^TAG
284 CTAG
BglII 855 AGATCT A^GATCT
4914 AGATCT
BsmBI 3473 CGTCTC CGTCTC(1/5)
4823 CGTCTC
BspLU11I 1981 ACATGT A^CATGT
2722 ACATGT
BsrBI 154 CCGCTC CCGCTC(-3/-3)
456 GAGCGG
BsrDI 2853 CATTGC GCAATG(2/0)
1218 GCAATG
BsrFI 3275 GCCGGC [GA]^CCGG[CT]
3597 GCCGGC
Cfr10I 3275 GCCGGC [GA]^CCGG[CT]
3597 GCCGGC
DrdI 263 GACCTGTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2809 GACATTGAAGTC
EcoRI 2747 GAATTC G^AATTC
4287 GAATTC
HaeII 845 GGCGCT [GA]GCGC^[CT]
3377 GGCGCT
KpnI 2840 GGTACC GGTAC^C
3310 GGTACC
KspI 292 CCGCGG CCGC^GG
636 CCGCGG
MaeI 234 CTAG C^TAG
284 CTAG
MfeI 391 CAATTG C^AATTG
3953 CAATTG
MluI 314 ACGCGT A^CGCGT
3539 ACGCGT
MseI 1034 TTAA T^TAA
2704 TTAA
MunI 391 CAATTG C^AATTG
3953 CAATTG
NaeI 3275 GCCGGC GCC^GGC
3597 GCCGGC
NdeI 1997 CATATG CA^TATG
3350 CATATG
NgoMIV 3275 GCCGGC G^CCGGC
3597 GCCGGC
SacI 1911 GAGCTC GAGCT^C
4972 GAGCTC
SacII 292 CCGCGG CCGC^GG
636 CCGCGG
Tru9I 1034 TTAA T^TAA
2704 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AccIII AciI AfaI AflIII
AluI AlwI AlwNI ApaI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcgI BcnI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspEI BspMI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MluNI MnlI
MroI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI XmnI