##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AgeI AhdI Alw44I ApaLI AscI
AseI AsnI Asp718I AspEI AspI
BbeI BbrPI BbsI BcgI BfrI
BpuAI BsaAI BsaBI BsaHI BsiEI
BsiWI BspDI BspHI Bst1107I BstBI
BstZ17I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco52I EcoT22I FseI
Hin1I HpaI KasI KpnI KspI
MamI MfeI MluI MunI NarI
NdeI NotI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
SexAI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I StuI SwaI Tth111I
XbaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 161 GTCTAC GT^[AC][GT]AC
AccIII 88 TCCGGA T^CCGGA
AcsI 2929 GAATTC [GA]^AATT[CT]
AflIII 2444 ACATGT A^C[GA][CT]GT
Aor51HI 482 AGCGCT AGC^GCT
ApoI 2929 GAATTC [GA]^AATT[CT]
Asp700I 1936 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 2514 TGGCCA TGG^CCA
BciVI 753 GTATCC GTATCC(6/5)
BglI 1024 GCCGAGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1978 AGATCT A^GATCT
BlpI 1000 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1000 GCTGAGC GC^T[ACGT]AGC
BsaI 3577 GAGACC GGTCTC(1/5)
BseAI 88 TCCGGA T^CCGGA
BsmI 938 GCATTC GAATGC(1/-1)
Bsp1407I 3584 TGTACA T^GTACA
BspEI 88 TCCGGA T^CCGGA
BspLU11I 2444 ACATGT A^CATGT
BsrGI 3584 TGTACA T^GTACA
BstAPI 1656 GCAGTAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 222 GGTGACC G^GT[ACGT]ACC
BstPI 222 GGTGACC G^GT[ACGT]ACC
CelII 1000 GCTGAGC GC^T[ACGT]AGC
Eco47III 482 AGCGCT AGC^GCT
EcoO65I 222 GGTGACC G^GT[ACGT]ACC
EcoRI 2929 GAATTC G^AATTC
EcoRV 3343 GATATC GAT^ATC
HincII 1187 GTCAAC GT[CT]^[GA]AC
HindII 1187 GTCAAC GT[CT]^[GA]AC
MluNI 2514 TGGCCA TGG^CCA
MroI 88 TCCGGA T^CCGGA
MscI 2514 TGGCCA TGG^CCA
NheI 2700 GCTAGC G^CTAGC
NruI 33 TCGCGA TCG^CGA
PaeR7I 172 CTCGAG C^TCGAG
SacI 84 GAGCTC GAGCT^C
SfiI 1023 GGCCGAGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 3584 TGTACA T^GTACA
SspI 3461 AATATT AAT^ATT
XhoI 172 CTCGAG C^TCGAG
XmnI 1936 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
ApaI 122 GGGCCC GGGCC^C
1031 GGGCCC
AspHI 84 GAGCTC G[AT]GC[AT]^C
3078 GTGCTC
AvrII 585 CCTAGG C^CTAGG
1229 CCTAGG
BanI 1210 GGTGCC G^G[CT][GA]CC
3488 GGTGCC
BclI 2587 TGATCA T^GATCA
3214 TGATCA
BlnI 585 CCTAGG C^CTAGG
1229 CCTAGG
BsaWI 88 TCCGGA [AT]^CCGG[AT]
157 TCCGGT
BsgI 3914 CTGCAC GTGCAG(16/14)
3203 GTGCAG
BsiHKAI 84 GAGCTC G[AT]GC[AT]^C
3078 GTGCTC
BsmBI 1123 CGTCTC CGTCTC(1/5)
4278 CGTCTC
BsrBI 130 CCGCTC CCGCTC(-3/-3)
1027 GAGCGG
BsrDI 3353 CATTGC GCAATG(2/0)
3904 CATTGC
BssSI 4231 CACGAG CACGAG(-5/-1)
342 CTCGTG
BstXI 3406 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4200 CCAGCACTTTGG
DraIII 378 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
2007 CACCTTGTG
FbaI 2587 TGATCA T^GATCA
3214 TGATCA
HindIII 639 AAGCTT A^AGCTT
1953 AAGCTT
MspA1I 971 CAGCTG C[AC]G^C[GT]G
1094 CAGCTG
PflMI 871 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1763 CCAGGGCCTGG
PvuII 971 CAGCTG CAG^CTG
1094 CAGCTG
SapI 47 GAAGAGC GCTCTTC(1/4)
745 GAAGAGC
ScaI 338 AGTACT AGT^ACT
1756 AGTACT
Van91I 871 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1763 CCAGGGCCTGG
XcmI 140 CCAGCGCCACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3639 CCACCCCGGGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI AvaI AvaII AviII
BamHI BanII BbvCI BbvI BcnI
BfaI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssHII
BssKI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI SphI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmaCI
XmaI