##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII AclI AcsI AflII AgeI
ApoI AscI AseI AsnI Asp700I
BciVI BclI BfrI BglII BsaBI
BsiWI BspDI BspLU11I BstBI ClaI
CpoI DraI EcoNI EcoRI EcoRV
FbaI HpaI MamI MfeI MluI
MseI MunI NheI NotI NruI
NspV PacI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SapI
SbfI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Tru9I
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 861 GGTACC G^GTACC
AccIII 2141 TCCGGA T^CCGGA
AflIII 597 ACACGT A^C[GA][CT]GT
AhdI 2667 GACACACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4106 AGCGCT AGC^GCT
Asp718I 861 GGTACC G^GTACC
AspEI 2667 GACACACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 3623 TGGCCA TGG^CCA
BbrPI 265 CACGTG CAC^GTG
BbvCI 1777 GCTGAGG CCTCAGC(-5/-2)
BseAI 2141 TCCGGA T^CCGGA
BsmI 3442 GAATGC GAATGC(1/-1)
Bsp1407I 1751 TGTACA T^GTACA
BspEI 2141 TCCGGA T^CCGGA
BspHI 2066 TCATGA T^CATGA
BsrGI 1751 TGTACA T^GTACA
BssSI 3846 CTCGTG CACGAG(-5/-1)
BstEII 3068 GGTCACC G^GT[ACGT]ACC
BstPI 3068 GGTCACC G^GT[ACGT]ACC
Eam1105I 2667 GACACACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4106 AGCGCT AGC^GCT
EcoO65I 3068 GGTCACC G^GT[ACGT]ACC
EcoT22I 587 ATGCAT ATGCA^T
FseI 4052 GGCCGGCC GGCCGG^CC
HindIII 2572 AAGCTT A^AGCTT
KpnI 861 GGTACC GGTAC^C
MluNI 3623 TGGCCA TGG^CCA
MroI 2141 TCCGGA T^CCGGA
MscI 3623 TGGCCA TGG^CCA
NdeI 2416 CATATG CA^TATG
NsiI 587 ATGCAT ATGCA^T
PaeR7I 3323 CTCGAG C^TCGAG
PmaCI 265 CACGTG CAC^GTG
PmlI 265 CACGTG CAC^GTG
RcaI 2066 TCATGA T^CATGA
SexAI 1640 ACCAGGT A^CC[AT]GGT
SspBI 1751 TGTACA T^GTACA
XhoI 3323 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AocI 320 CCTGAGG CC^T[ACGT]AGG
1416 CCTAAGG
ApaI 844 GGGCCC GGGCC^C
3258 GGGCCC
AspI 995 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1885 GACGCTGTC
AviII 2393 TGCGCA TGC^GCA
3982 TGCGCA
AvrII 464 CCTAGG C^CTAGG
3372 CCTAGG
BcgI 1999 CGAGTGCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3636 GCACTGTGGTCG
BlnI 464 CCTAGG C^CTAGG
3372 CCTAGG
BlpI 1095 GCTCAGC GC^T[ACGT]AGC
3764 GCTGAGC
Bpu1102I 1095 GCTCAGC GC^T[ACGT]AGC
3764 GCTGAGC
BsaI 137 GAGACC GGTCTC(1/5)
3086 GAGACC
BspMI 4084 ACCTGC ACCTGC(4/8)
3810 GCAGGT
BsrDI 2075 CATTGC GCAATG(2/0)
2374 CATTGC
Bst1107I 625 GTATAC GTA^TAC
2194 GTATAC
BstXI 205 CCAATCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2994 CCAGGATCCTGG
BstZ17I 625 GTATAC GTA^TAC
2194 GTATAC
Bsu36I 320 CCTGAGG CC^T[ACGT]AGG
1416 CCTAAGG
CelII 1095 GCTCAGC GC^T[ACGT]AGC
3764 GCTGAGC
DraIII 1816 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
1899 CACCGAGTG
DrdI 995 GACCCGGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2119 GACTGTCTTGTC
EagI 3805 CGGCCG C^GGCCG
4055 CGGCCG
EclXI 3805 CGGCCG C^GGCCG
4055 CGGCCG
Eco52I 3805 CGGCCG C^GGCCG
4055 CGGCCG
Eco81I 320 CCTGAGG CC^T[ACGT]AGG
1416 CCTAAGG
FspI 2393 TGCGCA TGC^GCA
3982 TGCGCA
NaeI 349 GCCGGC GCC^GGC
4053 GCCGGC
NcoI 1074 CCATGG C^CATGG
3739 CCATGG
NgoMIV 349 GCCGGC G^CCGGC
4053 GCCGGC
PflFI 995 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1885 GACGCTGTC
SacI 129 GAGCTC GAGCT^C
190 GAGCTC
SalI 1337 GTCGAC G^TCGAC
3903 GTCGAC
ScaI 403 AGTACT AGT^ACT
1949 AGTACT
SphI 589 GCATGC GCATG^C
2606 GCATGC
Tsp509I 1145 AATT ^AATT
2765 AATT
Tth111I 995 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1885 GACGCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaLI
AspHI AvaI AvaII BamHI BanI
BanII BbeI BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BsrBI BsrFI BsrI BssHII BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI StuI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI