##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AscI AseI AsnI AviII BbeI
BclI BfrI BglII BseAI BsiWI
BspDI BspEI BspHI BssHII Bst1107I
BstZ17I ClaI DraI EcoT22I FbaI
FseI FspI KasI MfeI MroI
MunI NarI NdeI NheI NotI
NruI NsiI PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI SexAI SfoI SgrAI SmlI
SnaBI SpeI SphI SspI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 3155 GGTACC G^GTACC
AccI 287 GTCGAC GT^[AC][GT]AC
Aor51HI 1152 AGCGCT AGC^GCT
Asp700I 529 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3155 GGTACC G^GTACC
AspI 3038 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1088 CCTAGG C^CTAGG
BamHI 1281 GGATCC G^GATCC
BlnI 1088 CCTAGG C^CTAGG
BsaWI 2381 ACCGGA [AT]^CCGG[AT]
BsiEI 3343 CGGCCG CG[GA][CT]^CG
BsmI 1642 GAATGC GAATGC(1/-1)
Bsp1407I 1673 TGTACA T^GTACA
BsrBI 2751 CCGCTC CCGCTC(-3/-3)
BsrGI 1673 TGTACA T^GTACA
BssSI 3599 CTCGTG CACGAG(-5/-1)
BstAPI 149 GCAGGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 4092 TTCGAA TT^CGAA
BstEII 2735 GGTGACC G^GT[ACGT]ACC
BstPI 2735 GGTGACC G^GT[ACGT]ACC
CpoI 2565 CGGACCG CG^G[AT]CCG
EagI 3343 CGGCCG C^GGCCG
EclXI 3343 CGGCCG C^GGCCG
Eco47III 1152 AGCGCT AGC^GCT
Eco52I 3343 CGGCCG C^GGCCG
EcoO65I 2735 GGTGACC G^GT[ACGT]ACC
EcoRI 1263 GAATTC G^AATTC
EcoRV 1609 GATATC GAT^ATC
HindIII 881 AAGCTT A^AGCTT
HpaI 1022 GTTAAC GTT^AAC
KpnI 3155 GGTACC GGTAC^C
KspI 2562 CCGCGG CCGC^GG
MseI 1023 TTAA T^TAA
NaeI 3122 GCCGGC GCC^GGC
NcoI 1080 CCATGG C^CATGG
NgoMIV 3122 GCCGGC G^CCGGC
NspV 4092 TTCGAA TT^CGAA
PflFI 3038 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsrII 2565 CGGACCG CG^G[AT]CCG
SacII 2562 CCGCGG CCGC^GG
SalI 287 GTCGAC G^TCGAC
SapI 3658 GCTCTTC GCTCTTC(1/4)
SbfI 3962 CCTGCAGG CCTGCA^GG
ScaI 1972 AGTACT AGT^ACT
SfiI 2398 GGCCTGCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 4092 TTCGAA TT^CGAA
Sse8387I 3962 CCTGCAGG CCTGCA^GG
SspBI 1673 TGTACA T^GTACA
Tru9I 1023 TTAA T^TAA
Tth111I 3038 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 529 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 1263 GAATTC [GA]^AATT[CT]
2359 AAATTC
AcyI 2342 GACGCC G[GA]^CG[CT]C
3323 GACGCC
ApoI 1263 GAATTC [GA]^AATT[CT]
2359 AAATTC
BbrPI 1461 CACGTG CAC^GTG
3208 CACGTG
BbsI 529 GAAGAC GAAGAC(2/6)
3493 GAAGAC
BbvCI 309 CCTCAGC CCTCAGC(-5/-2)
2948 CCTCAGC
BciVI 1529 GTATCC GTATCC(6/5)
3187 GTATCC
BlpI 415 GCTCAGC GC^T[ACGT]AGC
2792 GCTCAGC
Bpu1102I 415 GCTCAGC GC^T[ACGT]AGC
2792 GCTCAGC
BpuAI 529 GAAGAC GAAGAC(2/6)
3493 GAAGAC
BsaBI 1172 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3262 GATGGACATC
BsaHI 2342 GACGCC G[GA]^CG[CT]C
3323 GACGCC
BsmBI 187 CGTCTC CGTCTC(1/5)
3321 GAGACG
BspLU11I 349 ACATGT A^CATGT
1800 ACATGT
BsrDI 362 CATTGC GCAATG(2/0)
2021 GCAATG
BsrFI 3122 GCCGGC [GA]^CCGG[CT]
4394 GCCGGT
BstYI 1281 GGATCC [GA]^GATC[CT]
2204 AGATCC
CelII 415 GCTCAGC GC^T[ACGT]AGC
2792 GCTCAGC
Cfr10I 3122 GCCGGC [GA]^CCGG[CT]
4394 GCCGGT
DraIII 3134 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
3460 CACAGAGTG
DrdI 2647 GACCCCTGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2827 GACCCCCTTGTC
HaeII 1152 AGCGCT [GA]GCGC^[CT]
3696 GGCGCT
Hin1I 2342 GACGCC G[GA]^CG[CT]C
3323 GACGCC
MamI 1172 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3262 GATGGACATC
MflI 1281 GGATCC [GA]^GATC[CT]
2204 AGATCC
MluI 748 ACGCGT A^CGCGT
1517 ACGCGT
PmaCI 1461 CACGTG CAC^GTG
3208 CACGTG
PmlI 1461 CACGTG CAC^GTG
3208 CACGTG
TfiI 973 GATTC G^A[AT]TC
3743 GATTC
XhoII 1281 GGATCC [GA]^GATC[CT]
2204 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AfaI AflIII AhdI
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI AspEI AspHI AvaI
AvaII BalI BanI BanII BbvI
BcgI BcnI BfaI BglI BmyI
BpmI BsaAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI BstXI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI Eam1105I
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StuI StyI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XmaCI XmaI