################################## # NO CUT ENZYMES ( 57 enzymes) # ################################## AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AspEI AvrII BamHI BbvCI BclI BfrI BglII BlnI BspDI BspHI BspLU11I Bst1107I BstZ17I ClaI CpoI DraI Eam1105I Eco47III EcoT22I FbaI FseI HindIII HpaI KspI MfeI MluI MunI NdeI NotI NruI NsiI PacI PaeR7I PinAI PmeI PvuI RcaI RsrII SacII SalI SapI SbfI SfiI SgrAI SpeI SphI Sse8387I SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### Acc65I 2122 GGTACC G^GTACC AccI 3054 GTCTAC GT^[AC][GT]AC AccIII 2554 TCCGGA T^CCGGA AclI 2445 AACGTT AA^CGTT AflIII 2116 ACACGT A^C[GA][CT]GT AocI 3649 CCTCAGG CC^T[ACGT]AGG AseI 3061 ATTAAT AT^TAAT AsnI 3061 ATTAAT AT^TAAT Asp700I 2220 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2122 GGTACC G^GTACC BciVI 2273 GTATCC GTATCC(6/5) BfaI 1961 CTAG C^TAG BsaBI 1690 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 2554 TCCGGA [AT]^CCGG[AT] BseAI 2554 TCCGGA T^CCGGA BsiEI 1251 CGGCCG CG[GA][CT]^CG BsiWI 4235 CGTACG C^GTACG Bsp1407I 105 TGTACA T^GTACA BspEI 2554 TCCGGA T^CCGGA BsrGI 105 TGTACA T^GTACA BssHII 3784 GCGCGC G^CGCGC BstAPI 718 GCACTTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 209 TTCGAA TT^CGAA Bsu36I 3649 CCTCAGG CC^T[ACGT]AGG DraIII 719 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 1251 CGGCCG C^GGCCG EclXI 1251 CGGCCG C^GGCCG Eco52I 1251 CGGCCG C^GGCCG Eco81I 3649 CCTCAGG CC^T[ACGT]AGG EcoNI 949 CCTCCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 3318 GAATTC G^AATTC EcoRV 518 GATATC GAT^ATC HincII 524 GTCAAC GT[CT]^[GA]AC HindII 524 GTCAAC GT[CT]^[GA]AC KpnI 2122 GGTACC GGTAC^C MaeI 1961 CTAG C^TAG MamI 1690 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 2554 TCCGGA T^CCGGA NcoI 2399 CCATGG C^CATGG NheI 1960 GCTAGC G^CTAGC NspV 209 TTCGAA TT^CGAA PshAI 3221 GACTCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3061 ATTAAT AT^TAAT Psp1406I 2445 AACGTT AA^CGTT SacI 4318 GAGCTC GAGCT^C SexAI 2907 ACCTGGT A^CC[AT]GGT SfuI 209 TTCGAA TT^CGAA SnaBI 4237 TACGTA TAC^GTA SspBI 105 TGTACA T^GTACA SspI 4117 AATATT AAT^ATT XmnI 2220 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AatII 64 GACGTC GACGT^C 815 GACGTC AcsI 2693 GAATTT [GA]^AATT[CT] 3318 GAATTC ApoI 2693 GAATTT [GA]^AATT[CT] 3318 GAATTC AviII 2475 TGCGCA TGC^GCA 3793 TGCGCA BcgI 4115 CGAATATTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1506 GCAGCGGCCTCG BglI 1022 GCCTGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3554 GCCAAGTGGGC BsmI 1036 GAATGC GAATGC(1/-1) 1967 GAATGC BsrBI 1481 GAGCGG CCGCTC(-3/-3) 3643 GAGCGG DrdI 938 GACGGGACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2544 GACGTGAGGGTC FspI 2475 TGCGCA TGC^GCA 3793 TGCGCA NaeI 672 GCCGGC GCC^GGC 1249 GCCGGC NgoMIV 672 GCCGGC G^CCGGC 1249 GCCGGC ScaI 453 AGTACT AGT^ACT 1278 AGTACT SmaI 2249 CCCGGG CCC^GGG 2828 CCCGGG SmlI 2618 CTCAAG C^T[CT][GA]AG 3265 CTCAAG StuI 765 AGGCCT AGG^CCT 2773 AGGCCT XmaCI 2249 CCCGGG C^CCGGG 2828 CCCGGG XmaI 2249 CCCGGG C^CCGGG 2828 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 132 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AspI AvaI AvaII BalI BanI BanII BbeI BbrPI BbsI BbvI BcnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BssSI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII