##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AflII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AspEI AvrII BamHI
BbvCI BclI BfrI BglII BlnI
BspDI BspHI BspLU11I Bst1107I BstZ17I
ClaI CpoI DraI Eam1105I Eco47III
EcoT22I FbaI FseI HindIII HpaI
KspI MfeI MluI MunI NdeI
NotI NruI NsiI PacI PaeR7I
PinAI PmeI PvuI RcaI RsrII
SacII SalI SapI SbfI SfiI
SgrAI SpeI SphI Sse8387I SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 2122 GGTACC G^GTACC
AccI 3054 GTCTAC GT^[AC][GT]AC
AccIII 2554 TCCGGA T^CCGGA
AclI 2445 AACGTT AA^CGTT
AflIII 2116 ACACGT A^C[GA][CT]GT
AocI 3649 CCTCAGG CC^T[ACGT]AGG
AseI 3061 ATTAAT AT^TAAT
AsnI 3061 ATTAAT AT^TAAT
Asp700I 2220 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2122 GGTACC G^GTACC
BciVI 2273 GTATCC GTATCC(6/5)
BfaI 1961 CTAG C^TAG
BsaBI 1690 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2554 TCCGGA [AT]^CCGG[AT]
BseAI 2554 TCCGGA T^CCGGA
BsiEI 1251 CGGCCG CG[GA][CT]^CG
BsiWI 4235 CGTACG C^GTACG
Bsp1407I 105 TGTACA T^GTACA
BspEI 2554 TCCGGA T^CCGGA
BsrGI 105 TGTACA T^GTACA
BssHII 3784 GCGCGC G^CGCGC
BstAPI 718 GCACTTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 209 TTCGAA TT^CGAA
Bsu36I 3649 CCTCAGG CC^T[ACGT]AGG
DraIII 719 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1251 CGGCCG C^GGCCG
EclXI 1251 CGGCCG C^GGCCG
Eco52I 1251 CGGCCG C^GGCCG
Eco81I 3649 CCTCAGG CC^T[ACGT]AGG
EcoNI 949 CCTCCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3318 GAATTC G^AATTC
EcoRV 518 GATATC GAT^ATC
HincII 524 GTCAAC GT[CT]^[GA]AC
HindII 524 GTCAAC GT[CT]^[GA]AC
KpnI 2122 GGTACC GGTAC^C
MaeI 1961 CTAG C^TAG
MamI 1690 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2554 TCCGGA T^CCGGA
NcoI 2399 CCATGG C^CATGG
NheI 1960 GCTAGC G^CTAGC
NspV 209 TTCGAA TT^CGAA
PshAI 3221 GACTCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3061 ATTAAT AT^TAAT
Psp1406I 2445 AACGTT AA^CGTT
SacI 4318 GAGCTC GAGCT^C
SexAI 2907 ACCTGGT A^CC[AT]GGT
SfuI 209 TTCGAA TT^CGAA
SnaBI 4237 TACGTA TAC^GTA
SspBI 105 TGTACA T^GTACA
SspI 4117 AATATT AAT^ATT
XmnI 2220 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AatII 64 GACGTC GACGT^C
815 GACGTC
AcsI 2693 GAATTT [GA]^AATT[CT]
3318 GAATTC
ApoI 2693 GAATTT [GA]^AATT[CT]
3318 GAATTC
AviII 2475 TGCGCA TGC^GCA
3793 TGCGCA
BcgI 4115 CGAATATTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1506 GCAGCGGCCTCG
BglI 1022 GCCTGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3554 GCCAAGTGGGC
BsmI 1036 GAATGC GAATGC(1/-1)
1967 GAATGC
BsrBI 1481 GAGCGG CCGCTC(-3/-3)
3643 GAGCGG
DrdI 938 GACGGGACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2544 GACGTGAGGGTC
FspI 2475 TGCGCA TGC^GCA
3793 TGCGCA
NaeI 672 GCCGGC GCC^GGC
1249 GCCGGC
NgoMIV 672 GCCGGC G^CCGGC
1249 GCCGGC
ScaI 453 AGTACT AGT^ACT
1278 AGTACT
SmaI 2249 CCCGGG CCC^GGG
2828 CCCGGG
SmlI 2618 CTCAAG C^T[CT][GA]AG
3265 CTCAAG
StuI 765 AGGCCT AGG^CCT
2773 AGGCCT
XmaCI 2249 CCCGGG C^CCGGG
2828 CCCGGG
XmaI 2249 CCCGGG C^CCGGG
2828 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AspI
AvaI AvaII BalI BanI BanII
BbeI BbrPI BbsI BbvI BcnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BssSI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV NspI PflFI
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII