##################################
# NO CUT ENZYMES ( 43 enzymes) #
##################################
AclI AflII AgeI AscI AseI
AsnI Asp700I AvrII BciVI BfrI
BlnI BsiWI BspDI BspHI Bst1107I
BstBI BstXI BstZ17I ClaI DrdI
EcoRV EcoT22I MfeI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PinAI PmeI PshBI Psp1406I
PvuI RcaI SalI SfuI SnaBI
SspI SwaI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccIII 3272 TCCGGA T^CCGGA
Aor51HI 1007 AGCGCT AGC^GCT
BamHI 3703 GGATCC G^GATCC
BbrPI 1135 CACGTG CAC^GTG
BcgI 1638 CGAGCTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2441 TGATCA T^GATCA
BsaBI 2157 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3272 TCCGGA [AT]^CCGG[AT]
BseAI 3272 TCCGGA T^CCGGA
BsmBI 3894 GAGACG CGTCTC(1/5)
BsmI 558 GAATGC GAATGC(1/-1)
BspEI 3272 TCCGGA T^CCGGA
BspLU11I 3129 ACATGT A^CATGT
BssSI 568 CACGAG CACGAG(-5/-1)
BstAPI 3138 GCACCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CpoI 929 CGGACCG CG^G[AT]CCG
DraI 3507 TTTAAA TTT^AAA
Eco47III 1007 AGCGCT AGC^GCT
EcoRI 4091 GAATTC G^AATTC
FbaI 2441 TGATCA T^GATCA
FseI 3913 GGCCGGCC GGCCGG^CC
HincII 4243 GTTAAC GT[CT]^[GA]AC
HindII 4243 GTTAAC GT[CT]^[GA]AC
HindIII 4072 AAGCTT A^AGCTT
HpaI 4243 GTTAAC GTT^AAC
MamI 2157 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1550 ACGCGT A^CGCGT
MroI 3272 TCCGGA T^CCGGA
PaeR7I 1636 CTCGAG C^TCGAG
PmaCI 1135 CACGTG CAC^GTG
PmlI 1135 CACGTG CAC^GTG
PshAI 1529 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 929 CGGACCG CG^G[AT]CCG
SapI 2373 GCTCTTC GCTCTTC(1/4)
SpeI 3279 ACTAGT A^CTAGT
TfiI 3744 GATTC G^A[AT]TC
XbaI 4038 TCTAGA T^CTAGA
XhoI 1636 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AatII 2293 GACGTC GACGT^C
2529 GACGTC
Acc65I 2893 GGTACC G^GTACC
3227 GGTACC
AccI 2275 GTCTAC GT^[AC][GT]AC
4010 GTAGAC
AcsI 3861 AAATTC [GA]^AATT[CT]
4091 GAATTC
AflIII 1550 ACGCGT A^C[GA][CT]GT
3129 ACATGT
AlwI 3704 GATCC GGATC(4/5)
3703 GGATC
ApoI 3861 AAATTC [GA]^AATT[CT]
4091 GAATTC
Asp718I 2893 GGTACC G^GTACC
3227 GGTACC
AspI 2269 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2512 GACCGTGTC
BbvCI 1395 CCTCAGC CCTCAGC(-5/-2)
688 GCTGAGG
BglII 1505 AGATCT A^GATCT
2324 AGATCT
BlpI 441 GCTGAGC GC^T[ACGT]AGC
3107 GCTGAGC
Bpu1102I 441 GCTGAGC GC^T[ACGT]AGC
3107 GCTGAGC
BsaAI 1135 CACGTG [CT]AC^GT[GA]
1921 TACGTG
BsrDI 2129 GCAATG GCAATG(2/0)
4030 GCAATG
BssHII 228 GCGCGC G^CGCGC
230 GCGCGC
BstEII 1948 GGTAACC G^GT[ACGT]ACC
2139 GGTGACC
BstPI 1948 GGTAACC G^GT[ACGT]ACC
2139 GGTGACC
CelII 441 GCTGAGC GC^T[ACGT]AGC
3107 GCTGAGC
EcoO65I 1948 GGTAACC G^GT[ACGT]ACC
2139 GGTGACC
KpnI 2893 GGTACC GGTAC^C
3227 GGTACC
PflFI 2269 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2512 GACCGTGTC
ScaI 2084 AGTACT AGT^ACT
2573 AGTACT
SfiI 309 GGCCATCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
759 GGCCGTGCCGGCC
SgrAI 62 CACCGGCG C[GA]^CCGG[CT]G
189 CGCCGGTG
SphI 220 GCATGC GCATG^C
2460 GCATGC
Tth111I 2269 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2512 GACCGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 150 enzymes) #
#########################################
AccII AciI AcyI AfaI AhdI
AluI Alw44I AlwNI AocI ApaI
ApaLI AspEI AspHI AvaI AvaII
AviII BalI BanI BanII BbeI
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrBI BsrFI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EagI Eam1105I
EarI EclXI Eco52I Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI SacII Sau3AI
Sau96I SbfI ScrFI SexAI SfaNI
SfcI SfoI SmaI SmlI Sse8387I
SspBI StuI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI