##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AseI AsnI Asp718I AspI AvrII
BbrPI BclI BfrI BlnI BseAI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI Bst1107I BstBI
BstZ17I ClaI DraI DraIII DrdI
EcoRI EcoRV FbaI HpaI KpnI
KspI MfeI MluI MroI MunI
NdeI NotI NruI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI SacII
ScaI SexAI SfuI SgrAI SnaBI
SpeI SspBI SspI SwaI Tth111I
XbaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 3661 GACGTC GACGT^C
AcsI 3226 GAATTT [GA]^AATT[CT]
AflIII 2876 ACACGT A^C[GA][CT]GT
AhdI 4042 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2851 GTGCAC G^TGCAC
ApaLI 2851 GTGCAC G^TGCAC
ApoI 3226 GAATTT [GA]^AATT[CT]
AscI 143 GGCGCGCC GG^CGCGCC
AspEI 4042 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1235 TGCGCA TGC^GCA
BbvCI 1740 CCTCAGC CCTCAGC(-5/-2)
BfaI 1868 CTAG C^TAG
BlpI 3205 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3205 GCTGAGC GC^T[ACGT]AGC
BsaAI 874 TACGTG [CT]AC^GT[GA]
BsaBI 2709 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 3026 GCATTC GAATGC(1/-1)
BstEII 3519 GGTCACC G^GT[ACGT]ACC
BstPI 3519 GGTCACC G^GT[ACGT]ACC
CelII 3205 GCTGAGC GC^T[ACGT]AGC
CpoI 935 CGGACCG CG^G[AT]CCG
EagI 336 CGGCCG C^GGCCG
Eam1105I 4042 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 336 CGGCCG C^GGCCG
Eco52I 336 CGGCCG C^GGCCG
EcoO65I 3519 GGTCACC G^GT[ACGT]ACC
EcoT22I 1019 ATGCAT ATGCA^T
FseI 333 GGCCGGCC GGCCGG^CC
FspI 1235 TGCGCA TGC^GCA
HindIII 2955 AAGCTT A^AGCTT
MaeI 1868 CTAG C^TAG
MamI 2709 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 1867 GCTAGC G^CTAGC
NsiI 1019 ATGCAT ATGCA^T
PaeR7I 1005 CTCGAG C^TCGAG
RsrII 935 CGGACCG CG^G[AT]CCG
SacI 1999 GAGCTC GAGCT^C
SalI 1069 GTCGAC G^TCGAC
SbfI 3372 CCTGCAGG CCTGCA^GG
SphI 3833 GCATGC GCATG^C
Sse8387I 3372 CCTGCAGG CCTGCA^GG
Tsp509I 3227 AATT ^AATT
XhoI 1005 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 1069 GTCGAC GT^[AC][GT]AC
3190 GTCTAC
AocI 3258 CCTGAGG CC^T[ACGT]AGG
4243 CCTGAGG
Aor51HI 452 AGCGCT AGC^GCT
500 AGCGCT
Asp700I 864 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2943 GAAGTGTTTC
BamHI 824 GGATCC G^GATCC
2238 GGATCC
BbsI 2527 GTCTTC GAAGAC(2/6)
3496 GTCTTC
BciVI 1648 GGATAC GTATCC(6/5)
3263 GGATAC
BpuAI 2527 GTCTTC GAAGAC(2/6)
3496 GTCTTC
BsaI 1975 GAGACC GGTCTC(1/5)
1776 GGTCTC
BsaWI 3619 TCCGGT [AT]^CCGG[AT]
4544 ACCGGA
BsiEI 336 CGGCCG CG[GA][CT]^CG
774 CGACCG
BsmBI 296 GAGACG CGTCTC(1/5)
1616 GAGACG
BssSI 895 CACGAG CACGAG(-5/-1)
2997 CTCGTG
BstAPI 522 GCAGACGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3366 GCAGGCCCTGC
Bsu36I 3258 CCTGAGG CC^T[ACGT]AGG
4243 CCTGAGG
Eco47III 452 AGCGCT AGC^GCT
500 AGCGCT
Eco81I 3258 CCTGAGG CC^T[ACGT]AGG
4243 CCTGAGG
HincII 916 GTCAAC GT[CT]^[GA]AC
1069 GTCGAC
HindII 916 GTCAAC GT[CT]^[GA]AC
1069 GTCGAC
MseI 3113 TTAA T^TAA
4471 TTAA
NaeI 334 GCCGGC GCC^GGC
1685 GCCGGC
NgoMIV 334 GCCGGC G^CCGGC
1685 GCCGGC
NspI 1427 GCATGT [GA]CATG^[CT]
3833 GCATGC
SapI 546 GAAGAGC GCTCTTC(1/4)
2034 GCTCTTC
SfiI 237 GGCCATGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1148 GGCCCCCTTGGCC
SmlI 1005 CTCGAG C^T[CT][GA]AG
2563 CTCAAG
StuI 461 AGGCCT AGG^CCT
4425 AGGCCT
Tru9I 3113 TTAA T^TAA
4471 TTAA
XmnI 864 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2943 GAAGTGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbvI BcgI BcnI BglI BglII
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssHII BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV PflMI
PleI PpuMI PshAI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI StyI TaqI
TfiI TseI Tsp45I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI