##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII AccI AclI AcsI AflII
AflIII AgeI AhdI Alw44I ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AvrII BbrPI BcgI
BclI BfrI BlnI BsaBI BsiEI
BsiWI BsmBI BsmI BspDI BspLU11I
Bst1107I BstBI BstZ17I ClaI DraI
DrdI EagI Eam1105I EclXI Eco52I
EcoRI EcoRV FbaI FseI HindIII
HpaI KspI MamI MfeI MluI
MseI MunI NdeI NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI SacII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SpeI SphI Sse8387I SspI SwaI
Tru9I Tsp509I Tth111I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 1944 GGTACC G^GTACC
AccIII 33 TCCGGA T^CCGGA
Aor51HI 2769 AGCGCT AGC^GCT
Asp718I 1944 GGTACC G^GTACC
AviII 2598 TGCGCA TGC^GCA
BamHI 3180 GGATCC G^GATCC
BciVI 3240 GTATCC GTATCC(6/5)
BglII 2343 AGATCT A^GATCT
BlpI 2144 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2144 GCTCAGC GC^T[ACGT]AGC
BsaAI 1122 TACGTA [CT]AC^GT[GA]
BseAI 33 TCCGGA T^CCGGA
Bsp1407I 3213 TGTACA T^GTACA
BspEI 33 TCCGGA T^CCGGA
BspHI 1621 TCATGA T^CATGA
BsrGI 3213 TGTACA T^GTACA
BssHII 398 GCGCGC G^CGCGC
BstEII 1358 GGTCACC G^GT[ACGT]ACC
BstPI 1358 GGTCACC G^GT[ACGT]ACC
CelII 2144 GCTCAGC GC^T[ACGT]AGC
Eco47III 2769 AGCGCT AGC^GCT
EcoNI 181 CCTACCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1358 GGTCACC G^GT[ACGT]ACC
EcoT22I 3332 ATGCAT ATGCA^T
FspI 2598 TGCGCA TGC^GCA
KpnI 1944 GGTACC GGTAC^C
MroI 33 TCCGGA T^CCGGA
NsiI 3332 ATGCAT ATGCA^T
PshAI 1792 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1621 TCATGA T^CATGA
SnaBI 1122 TACGTA TAC^GTA
SspBI 3213 TGTACA T^GTACA
StuI 667 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BbeI 170 GGCGCC GGCGC^C
1938 GGCGCC
BsaWI 33 TCCGGA [AT]^CCGG[AT]
2933 TCCGGT
BsgI 1979 CTGCAC GTGCAG(16/14)
3551 CTGCAC
BsrDI 1591 CATTGC GCAATG(2/0)
3446 CATTGC
CpoI 1521 CGGTCCG CG^G[AT]CCG
3018 CGGTCCG
DraIII 1067 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG
1812 CACGGGGTG
HincII 3046 GTTGAC GT[CT]^[GA]AC
3735 GTTGAC
HindII 3046 GTTGAC GT[CT]^[GA]AC
3735 GTTGAC
KasI 170 GGCGCC G^GCGCC
1938 GGCGCC
NaeI 879 GCCGGC GCC^GGC
3277 GCCGGC
NarI 170 GGCGCC GG^CGCC
1938 GGCGCC
NgoMIV 879 GCCGGC G^CCGGC
3277 GCCGGC
NspI 1341 ACATGC [GA]CATG^[CT]
3555 ACATGC
PvuII 3245 CAGCTG CAG^CTG
3353 CAGCTG
RsrII 1521 CGGTCCG CG^G[AT]CCG
3018 CGGTCCG
SapI 2005 GCTCTTC GCTCTTC(1/4)
3463 GCTCTTC
SfoI 170 GGCGCC GGC^GCC
1938 GGCGCC
SmlI 1505 CTTGAG C^T[CT][GA]AG
2924 CTCAAG
TfiI 578 GAATC G^A[AT]TC
1222 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StyI
TaqI TseI Tsp45I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI