################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI Aor51HI AscI AseI AsnI Asp700I Asp718I AspI BamHI BbeI BciVI BclI BfrI BglII BlpI Bpu1102I BsaBI BseAI BsiWI BspDI BspEI BsrDI BssHII Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I CelII ClaI CpoI DraIII DrdI Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KasI KpnI MamI MfeI MflI MluI MroI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmeI PshBI Psp1406I PvuI RsrII SacI SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI Tth111I XbaI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AcsI 1882 GAATTT [GA]^AATT[CT] AcyI 429 GACGCC G[GA]^CG[CT]C AhdI 1261 GACCGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 359 GTGCAC G^TGCAC AlwI 246 GATCC GGATC(4/5) AocI 1139 CCTCAGG CC^T[ACGT]AGG ApaLI 359 GTGCAC G^TGCAC ApoI 1882 GAATTT [GA]^AATT[CT] AspEI 1261 GACCGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 382 TGCGCA TGC^GCA BalI 205 TGGCCA TGG^CCA BbrPI 3128 CACGTG CAC^GTG BbvCI 420 GCTGAGG CCTCAGC(-5/-2) BcgI 332 CGAGATGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaAI 3128 CACGTG [CT]AC^GT[GA] BsaHI 429 GACGCC G[GA]^CG[CT]C BsaWI 193 ACCGGA [AT]^CCGG[AT] BsiEI 349 CGGCCG CG[GA][CT]^CG BsmBI 1014 CGTCTC CGTCTC(1/5) BsmI 338 GAATGC GAATGC(1/-1) BspLU11I 355 ACATGT A^CATGT BstAPI 2803 GCAGGGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 1139 CCTCAGG CC^T[ACGT]AGG DraI 1488 TTTAAA TTT^AAA EagI 349 CGGCCG C^GGCCG Eam1105I 1261 GACCGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 349 CGGCCG C^GGCCG Eco52I 349 CGGCCG C^GGCCG Eco81I 1139 CCTCAGG CC^T[ACGT]AGG FspI 382 TGCGCA TGC^GCA HaeII 1024 AGCGCC [GA]GCGC^[CT] Hin1I 429 GACGCC G[GA]^CG[CT]C MluNI 205 TGGCCA TGG^CCA MscI 205 TGGCCA TGG^CCA NcoI 1685 CCATGG C^CATGG PaeR7I 2678 CTCGAG C^TCGAG PleI 1694 GACTC GAGTC(4/5) PmaCI 3128 CACGTG CAC^GTG PmlI 3128 CACGTG CAC^GTG PshAI 1694 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 232 GCTCTTC GCTCTTC(1/4) ScaI 292 AGTACT AGT^ACT SspI 1513 AATATT AAT^ATT XhoI 2678 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AflIII 97 ACGTGT A^C[GA][CT]GT 355 ACATGT AvaI 2664 CTCGGG C^[CT]CG[GA]G 2678 CTCGAG AvrII 901 CCTAGG C^CTAGG 1714 CCTAGG BbsI 2308 GTCTTC GAAGAC(2/6) 2603 GTCTTC BlnI 901 CCTAGG C^CTAGG 1714 CCTAGG BpuAI 2308 GTCTTC GAAGAC(2/6) 2603 GTCTTC BsoBI 2664 CTCGGG C^[CT]CG[GA]G 2678 CTCGAG Bsp1407I 100 TGTACA T^GTACA 1477 TGTACA BspHI 621 TCATGA T^CATGA 2272 TCATGA BsrBI 230 CCGCTC CCGCTC(-3/-3) 3224 GAGCGG BsrGI 100 TGTACA T^GTACA 1477 TGTACA BssSI 513 CACGAG CACGAG(-5/-1) 782 CTCGTG DpnII 246 GATC ^GATC 464 GATC HgaI 429 GACGC GACGC(5/10) 376 GCGTC KspI 568 CCGCGG CCGC^GG 652 CCGCGG MboI 246 GATC ^GATC 464 GATC MslI 92 CACCAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 778 CACACTCGTG NdeII 246 GATC ^GATC 464 GATC NspI 355 ACATGT [GA]CATG^[CT] 666 GCATGT PstI 546 CTGCAG CTGCA^G 941 CTGCAG RcaI 621 TCATGA T^CATGA 2272 TCATGA SacII 568 CCGCGG CCGC^GG 652 CCGCGG Sau3AI 246 GATC ^GATC 464 GATC SfaNI 395 GATGC GCATC(5/9) 226 GCATC SspBI 100 TGTACA T^GTACA 1477 TGTACA StuI 1791 AGGCCT AGG^CCT 2562 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 93 enzymes) # ######################################### AccII AciI AfaI AluI AlwNI ApaI AspHI AvaII BanI BanII BbvI BcnI BfaI BglI BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NaeI NciI NgoMIV NlaIII NlaIV PflMI PpuMI PvuII RsaI Sau96I ScrFI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI