##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspI BamHI
BbeI BciVI BclI BfrI BglII
BlpI Bpu1102I BsaBI BseAI BsiWI
BspDI BspEI BsrDI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I CelII ClaI CpoI DraIII
DrdI Eco47III EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KasI KpnI MamI
MfeI MflI MluI MroI MunI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacI SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
XbaI XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AcsI 1882 GAATTT [GA]^AATT[CT]
AcyI 429 GACGCC G[GA]^CG[CT]C
AhdI 1261 GACCGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 359 GTGCAC G^TGCAC
AlwI 246 GATCC GGATC(4/5)
AocI 1139 CCTCAGG CC^T[ACGT]AGG
ApaLI 359 GTGCAC G^TGCAC
ApoI 1882 GAATTT [GA]^AATT[CT]
AspEI 1261 GACCGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 382 TGCGCA TGC^GCA
BalI 205 TGGCCA TGG^CCA
BbrPI 3128 CACGTG CAC^GTG
BbvCI 420 GCTGAGG CCTCAGC(-5/-2)
BcgI 332 CGAGATGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 3128 CACGTG [CT]AC^GT[GA]
BsaHI 429 GACGCC G[GA]^CG[CT]C
BsaWI 193 ACCGGA [AT]^CCGG[AT]
BsiEI 349 CGGCCG CG[GA][CT]^CG
BsmBI 1014 CGTCTC CGTCTC(1/5)
BsmI 338 GAATGC GAATGC(1/-1)
BspLU11I 355 ACATGT A^CATGT
BstAPI 2803 GCAGGGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1139 CCTCAGG CC^T[ACGT]AGG
DraI 1488 TTTAAA TTT^AAA
EagI 349 CGGCCG C^GGCCG
Eam1105I 1261 GACCGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 349 CGGCCG C^GGCCG
Eco52I 349 CGGCCG C^GGCCG
Eco81I 1139 CCTCAGG CC^T[ACGT]AGG
FspI 382 TGCGCA TGC^GCA
HaeII 1024 AGCGCC [GA]GCGC^[CT]
Hin1I 429 GACGCC G[GA]^CG[CT]C
MluNI 205 TGGCCA TGG^CCA
MscI 205 TGGCCA TGG^CCA
NcoI 1685 CCATGG C^CATGG
PaeR7I 2678 CTCGAG C^TCGAG
PleI 1694 GACTC GAGTC(4/5)
PmaCI 3128 CACGTG CAC^GTG
PmlI 3128 CACGTG CAC^GTG
PshAI 1694 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 232 GCTCTTC GCTCTTC(1/4)
ScaI 292 AGTACT AGT^ACT
SspI 1513 AATATT AAT^ATT
XhoI 2678 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 97 ACGTGT A^C[GA][CT]GT
355 ACATGT
AvaI 2664 CTCGGG C^[CT]CG[GA]G
2678 CTCGAG
AvrII 901 CCTAGG C^CTAGG
1714 CCTAGG
BbsI 2308 GTCTTC GAAGAC(2/6)
2603 GTCTTC
BlnI 901 CCTAGG C^CTAGG
1714 CCTAGG
BpuAI 2308 GTCTTC GAAGAC(2/6)
2603 GTCTTC
BsoBI 2664 CTCGGG C^[CT]CG[GA]G
2678 CTCGAG
Bsp1407I 100 TGTACA T^GTACA
1477 TGTACA
BspHI 621 TCATGA T^CATGA
2272 TCATGA
BsrBI 230 CCGCTC CCGCTC(-3/-3)
3224 GAGCGG
BsrGI 100 TGTACA T^GTACA
1477 TGTACA
BssSI 513 CACGAG CACGAG(-5/-1)
782 CTCGTG
DpnII 246 GATC ^GATC
464 GATC
HgaI 429 GACGC GACGC(5/10)
376 GCGTC
KspI 568 CCGCGG CCGC^GG
652 CCGCGG
MboI 246 GATC ^GATC
464 GATC
MslI 92 CACCAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
778 CACACTCGTG
NdeII 246 GATC ^GATC
464 GATC
NspI 355 ACATGT [GA]CATG^[CT]
666 GCATGT
PstI 546 CTGCAG CTGCA^G
941 CTGCAG
RcaI 621 TCATGA T^CATGA
2272 TCATGA
SacII 568 CCGCGG CCGC^GG
652 CCGCGG
Sau3AI 246 GATC ^GATC
464 GATC
SfaNI 395 GATGC GCATC(5/9)
226 GCATC
SspBI 100 TGTACA T^GTACA
1477 TGTACA
StuI 1791 AGGCCT AGG^CCT
2562 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
ApaI AspHI AvaII BanI BanII
BbvI BcnI BfaI BglI BmyI
BpmI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboII MnlI MseI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NgoMIV NlaIII NlaIV PflMI
PpuMI PvuII RsaI Sau96I ScrFI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI